血塞通胶囊通过抑制炎症因子表达对心肌细胞缺氧损伤的保护作用

韩勇; 刘新文; 王超海; 杨欣蔚; 李文华; 景江

doi:10.16766/j.cnki.issn.1674-4152.003019

血塞通胶囊通过抑制炎症因子表达对心肌细胞缺氧损伤的保护作用

doi: 10.16766/j.cnki.issn.1674-4152.003019

1.
绍兴文理学院附属医院药剂科，浙江绍兴 312000
2.
绍兴文理学院元培学院, 绿色化学与制药工程重点实验室, 浙江绍兴 312000
3.
绍兴文理学院附属医院康复医学科

基金项目:

浙江省教育厅一般科研项目 Y202146979

浙江省绍兴市卫生健康科技计划项目 2022KY066

详细信息

通讯作者:
刘新文，E-mail: liuxinwen822@163.com

中图分类号: R542.2 R331.31
计量
- 文章访问数: 165
- HTML全文浏览量: 56
- PDF下载量: 4
- 被引次数: 0
出版历程
- 收稿日期: 2022-12-18
- 网络出版日期: 2023-08-26

Protective effect of Xuesaitong capsule on myocardial cell hypoxia injury by inhibiting the expression of inflammatory factors

1.
Department of Pharmacy, Affiliated Hospital of Shaoxing University of Arts and Sciences, Shaoxing, Zhejiang 312000, China

摘要

摘要: 目的通过建立心肌损伤大鼠模型，并应用血塞通胶囊处理损伤的心肌细胞，探讨血塞通胶囊对心肌细胞氧化损伤的保护作用。方法将25只SD雄性大鼠采用随机数字表法分为空白对照组、心肌细胞缺氧/复氧(H/R)模型组和血塞通胶囊低、中、高剂量组，每组5只，取每组大鼠心肌细胞进行培养，各组心肌细胞凋亡率采用流式细胞仪检测，各组炎症因子[白细胞介素-6(IL-6)、IL-8、肿瘤坏死因子-α(TNF-α)]水平和氧化应激指标[乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)]水平采用ELISA测定，应用Western blotting法检测核因子κB(NF-κB)表达量。结果 H/R组心肌细胞凋亡率[(22.85±1.78)%]高于对照组[(3.45±0.42)%]及血塞通胶囊低、中、高剂量组[(10.25±2.02)%、(8.96±1.23)%、(5.88±1.45)%]，均P＜0.05；H/R组血清IL-6、TNF-α、LDH、MDA及NF-κB表达量较对照组及血塞通胶囊低、中、高剂量组显著提升(P＜0.05)，而H/R组SOD活力较对照组及血塞通胶囊低、中、高剂量组显著下降(均P＜0.05)。结论血塞通胶囊对心肌细胞H/R损伤具有保护作用，其保护机制可能与其能抑制NF-κB基因过表达有关。
- 血塞通胶囊 /
- 心肌损伤 /
- 炎症因子 /
- 心肌细胞 /
- 氧化损伤
Abstract: Objective To explore the protective effect of Xuesaitong capsule on oxidative damage of myocardial cells by establishing a mouse model of myocardial injury and treating the injured myocardial cells with Xuesaitong capsule. Methods A total of 25 SD rats were randomly divided into blank control group, myocardial cell hypoxia/reoxygenation (H/R) model group, low, medium and high dose groups of Xuesaitong capsule by rasom number table method, with 5 rats in each group. The myocardial cells of each group were cultured. The apoptosis rate of myocardial cells in each group were detected by flow cytometry. The inflammatory factors [interleukin-6 (IL-6), IL-8, tumor necrosis factor-α (TNF-α)] and oxidative stress indicators [lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD)] in each group were measured by ELISA, and NF-κB expression were detected by Western blotting. Results The apoptosis rate of myocardial cells in H/R group [(22.85±1.78)%] were higher than control group [(3.45±0.42)%] and the low, medium and high dose groups of Xuesaitong capsule [(10.25±2.02)%, (8.96±1.23)%, (5.88±1.45)%]. The serum IL-6, TNF-α, LDH, MDA and NF-κB in H/R group were significantly higher than control group and low, medium and high dose groups of Xuesaitong capsule (P < 0.05), while the activity of SOD in H/R group were significantly lower than that of control group and low, medium and high dose groups of Xuesaitong capsule (all P < 0.05). Conclusion Xuesaitong capsule has protective effect on H/R injury of myocardial cells, and its protective mechanism may be related to its inhibition of NF-κB gene overexpression.
- Xuesaitong capsule /
- Myocardial injury /
- Inflammatory factors /
- Myocardial cells /
- Oxidative damage

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图 1 倒置显微镜下大鼠心肌细胞

注：A为放大100倍，B为放大400倍。

Figure 1. Image of rat cardiomyocytes under inverted microscope

下载: 全尺寸图片幻灯片

图 2 各组大鼠心肌细胞中NF-κB蛋白表达的免疫印迹图

注：1为空白对照组；2为H/R组；3为血塞通胶囊低剂量组；4为血塞通中剂量组；5为血塞通高剂量组。

Figure 2. Western blotting of NF-κB protein expression in rat cardiomyocytes in each group

下载: 全尺寸图片幻灯片

表 1 各组大鼠血清炎症因子水平比较(x±s，μg/L)

Table 1. Comparison of serum inflammatory factor levels in SD rats of each group (x±s, μg/L)

组别	只数	IL-6	IL-8	TNF-α
空白对照组	5	22.52±3.98	32.98±4.55	4.26±0.98
H/R组	5	105.23±12.52^a	115.23±9.89^a	18.12±4.23^a
血塞通胶囊低剂量组	5	81.12±9.58^ab	92.12±10.22^ab	12.25±3.98^ab
血塞通胶囊中剂量组	5	65.88±12.02^abc	71.02±13.98^abc	9.10±4.10^abc
血塞通胶囊高剂量组	5	42.12±5.98^abcd	52.96±6.78^abcd	6.77±2.02^abcd
F值		45.224	36.593	34.102
P值		＜0.001	＜0.001	＜0.001
注：与空白对照组比较，^aP＜0.05；与H/R组比较，^bP＜0.05；与血塞通胶囊低剂量组比较，^cP＜0.05；与血塞通胶囊中剂量组比较，^dP＜0.05。

下载: 导出CSV

表 2 各组大鼠心肌细胞氧化应激指标比较(x±s)

Table 2. Comparison of oxidative stress indexes of rat myocardial cells in each group (x±s)

组别	只数	LDH(U/L)	MDA(nmol/mg)	SOD(μ/mg)
空白对照组	5	14.25±3.12	10.10±1.89	38.96±4.98
H/R组	5	68.95±4.10^a	42.25±3.29^a	12.10±2.02^a
血塞通胶囊低剂量组	5	52.11±3.56^ab	25.98±4.52^ab	20.12±4.56^ab
血塞通胶囊中剂量组	5	32.10±4.02^abc	18.96±3.66^abc	25.98±3.98^abc
血塞通胶囊高剂量组	5	22.98±3.44^abcd	13.23±2.98^abcd	28.92±4.21^abcd
F值		82.112	75.263	65.883
P值		＜0.001	＜0.001	＜0.001
注：与空白对照组比较，^aP＜0.05；与H/R组比较，^bP＜0.05；与血塞通胶囊低剂量组比较，^cP＜0.05；与血塞通胶囊中剂量组比较，^dP＜0.05。

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