circPRKCI和miR-545在结直肠癌中的表达及相关性研究

刘景华; 王海玲; 赵阳; 马剑锋

doi:10.16766/j.cnki.issn.1674-4152.003406

circPRKCI和miR-545在结直肠癌中的表达及相关性研究

doi: 10.16766/j.cnki.issn.1674-4152.003406

1.
郑州市第七人民医院消化内科，河南郑州 450000
2.
河南省肿瘤医院肿瘤内科，河南郑州 450000
3.
郑州市第七人民医院普通外科

基金项目:

河南省医学科技攻关计划项目 LHGJ20220205

详细信息

通讯作者:
刘景华，E-mail：liujinghua1986115@163.com

中图分类号: R735.35 R735.37 R730.43
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- 文章访问数: 92
- HTML全文浏览量: 35
- PDF下载量: 9
- 被引次数: 0
出版历程
- 收稿日期: 2023-09-05
- 网络出版日期: 2024-05-27

The expression and correlation between circPRKCI and miR-545 in colorectal cancer

1.
Department of Gastroenterology, the Seventh People ' s Hospital of Zhengzhou, Zhengzhou, Henan 450000, China

摘要

摘要: 目的探讨结直肠癌组织环状RNA PRKCI(circPRKCI)、微小RNA-545(miR-545)的表达及其对预后的影响。方法收集2013年3月—2018年4月在郑州市第七人民医院行手术切除治疗的102例结直肠癌患者的癌组织和癌旁组织标本，分析circPRKCI、miR-545的表达与结直肠癌患者临床病理参数及预后的关系。结果与癌旁组织相比，circPRKCI在结直肠癌组织中的表达显著上调(1.47±0.69 vs. 0.82±0.42, t=8.644, P＜0.05)，miR-545在结直肠癌组织中的表达显著下调(0.86±0.55 vs. 1.60±0.66, t=8.908, P＜0.05)。有淋巴结转移、TNM分期Ⅲ~Ⅳ期患者circPRKCI表达升高, miR-545表达下降(P < 0.05)。circPRKCI高表达、miR-545低表达患者的预后较差(P＜0.05)，circPRKCI和miR-545是结直肠癌患者预后不良的独立影响因素，两者表达呈负相关关系。结论 circPRKCI、miR-545与结直肠癌患者的不良病理参数和预后相关，circPRKCI可能调控miR-545参与结直肠癌的进展。
- 结直肠癌 /
- 微小RNA-545 /
- 环状RNA PRKCI /
- 临床病理参数
Abstract: Objective To investigate the expression of circular RNA PRKCI (circPRKCI) and microrNA-545 (miR-545) in colorectal cancer tissues and their effects on prognosis. Methods From March 2013 to April 2018, 102 patients with colorectal cancer underwent surgical resection in Zhengzhou Seventh People ' s Hospital were collected. The expression levels of circPRKCI and miR-545 in colorectal cancer patients with different clinicopathological characteristics were analyzed. The effects of circPRKCI and miR-545 expression levels on the prognosis of patients were analyzed by Cox regression and survival curve. Results The expression of circPRKCI was significantly up-regulated in colorectal cancer tissues compared to paracancer tissues (1.47±0.69 vs. 0.82±0.42, t=8.644, P < 0.05). Conversely, miR-545 expression was significantly down-regulated in colorectal cancer tissues (0.86±0.55 vs. 1.60±0.66, t=8.908, P < 0.05). The expression of circPRKCI increased and the expression of miR-545 decreased in patients with lymph node metastasis and TNM stage Ⅲ-Ⅳ (P < 0.05). Furthermore, patients with high expression of circPRKCI and low expression of miR-545 exhibited poor prognosis (P < 0.05). Both circPRKCI and miR-545 were identified as independent risk factors for poor prognosis in patients with colorectal cancer, and their expression showed a negative correlation. Conclusion The expression of circPRKCI and miR-545 is related to the adverse pathological parameters and prognosis in patients with colorectal cancer, suggesting a potential regulatory role of circPRKCI in the progression of colorectal cancer involving miR-545.
- Colorectal cancer /
- MicroRNA-545 /
- Circular RNA PRKCI /
- Clinicopathological parameters

HTML全文

图 1 不同circPRKCI和miR-545表达水平结直肠癌患者总生存期的Kaplan-Meier生存曲线

Figure 1. Kaplan-Meier survival curve depicting the overall survival of colorectal cancer patients based on varying expression levels of circPRKCI and miR-545

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表 1 结直肠癌组织和癌旁组织中circPRKCI、miR-545表达水平比较(x±s)

Table 1. Comparison of circPRKCI and miR-545 expression levels between colorectal cancer tissue and paracancer tissue

组别	例数	circPRKCI相对表达量	miR-545相对表达量
结直肠癌组织	102	1.47±0.69	0.86±0.55
癌旁组织	102	0.82±0.42	1.60±0.66
t值		8.644	8.908
P值		< 0.001	< 0.001

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表 2 不同临床病理特征结直肠癌患者circPRKCI、miR-545表达水平比较

Table 2. Comparison of circPRKCI and miR-545 expression levels in colorectal cancer patients with different clinicopathological characteristics

项目	类别	例数	circPRKCI相对表达量(x±s)	t值	P值	miR-545相对表达量[M(P₂₅, P₇₅)]	Z值	P值
性别	男性	62	1.51±0.48	0.986	0.327	0.83(0.42, 1.18)	1.028	0.304
	女性	40	1.41±0.53			0.85(0.57, 1.27)
年龄(岁)	≤60	32	1.43±0.51	0.625	0.533	0.84(0.61, 1.27)	0.494	0.621
	＞60	70	1.49±0.42			0.81(0.41, 1.31)
肿瘤直径(cm)	≤5	56	1.39±0.50	1.623	0.108	0.91(0.53, 1.28)	1.544	0.123
	＞5	46	1.57±0.62			0.78(0.28, 1.25)
淋巴结转移	有	55	1.62±0.48	3.085	0.003	0.81(0.28, 1.17)	2.756	0.006
	无	47	1.29±0.60			0.94(0.70, 1.44)
T分期	T1+T2	58	1.37±0.62	1.817	0.072	1.02(0.58, 1.36)	1.618	0.106
	T3+T4	44	1.60±0.65			0.83(0.39, 1.22)
TNM分期	Ⅰ~Ⅱ	55	1.33±0.49	2.924	0.004	1.01(0.54, 1.40)	2.642	0.008
	Ⅲ~Ⅳ	47	1.62±0.51			0.73(0.30, 1.19)

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表 3 不同circPRKCI、miR-545表达水平结直肠癌患者5年生存率比较

Table 3. Comparison of 5-year survival rates among colorectal cancer patients with different expression levels of circPRKCI and miR-545

组别	例数	5年生存[例(%)]	log-rank χ²值	P值
circPRKCI高表达组	56	20(35.71)	6.713	0.010
circPRKCI低表达组	46	30(65.22)
miR-545高表达组	40	24(60.00)	4.162	0.041
miR-545低表达组	62	26(41.94)

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表 4 结直肠癌患者预后不良的影响因素分析

Table 4. Analysis of factors influencing poor prognosis in colorectal cancer patients

变量	β	SE	Waldχ²	P值	HR值	HR的95% CI
性别	0.141	0.085	2.734	0.098	1.151	0.974~1.361
年龄	0.201	0.128	2.483	0.115	1.223	0.952~1.570
肿瘤直径	0.710	0.412	2.966	0.085	2.034	0.907~4.563
淋巴结转移	0.972	0.348	7.806	0.005	2.642	1.337~5.227
T分期	0.772	0.412	3.507	0.061	2.164	0.965~4.855
TNM分期	1.145	0.419	7.475	0.006	3.143	1.383~7.141
circPRKCI表达	0.921	0.400	5.303	0.021	2.511	1.147~5.501
miR-545表达	-0.989	0.465	4.528	0.033	0.372	0.150~0.925
注：变量赋值如下，性别(男性=1，女性=0)；年龄(≥60岁=1，＜60岁=0)；肿瘤直径(≥5 cm=1，＜5 cm=0)；淋巴结转移(有=1，无=0)；T分期(T3+T4=1，T1+T2=0)；TNM分期(Ⅲ~Ⅳ期=1，Ⅰ~Ⅱ期=0)；circPRKCI表达(高表达=1，低表达=0)；miR-545表达(高表达=1，低表达=0)。

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