Protective effect and mechanism of Alpiniae oxyphylla fructus on myocardial injury after exhaustive exercise in mice
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摘要:
目的 本研究旨在建立力竭运动引起的运动性心肌损伤小鼠模型,通过观察急性力竭运动后小鼠心肌损伤指标的变化,探讨益智仁预处理对力竭运动后心肌损伤的干预作用及其可能的机制。 方法 采用简单随机分组将小鼠分为5个实验组:对照组(Con,n=6)、益智仁组(Yz,n=6)、力竭组(EE,n=6)、力竭运动模型+益智仁低剂量组(EE+EYz,n=6)以及力竭运动模型+益智仁高剂量组(EE+HYz,n=6),所有小鼠均接受为期6周的特定运动干预与药物喂养,随后处死并收集小鼠心肌损伤相关生物标志物数据。 结果 在力竭次数方面,EE+HYz组和EE+EYz组相较于EE组差异有统计学意义(P<0.05)。在体重变化方面,EE+HYz组、Con组与Yz组,以及EE+HYz组与EE组比较差异均有统计学意义(P<0.05)。EE+HYz组的TNF-α水平与EE组相比显著降低(P<0.05);5组心肌型肌红蛋白(MYO)比较差异有统计学意义(P<0.05);其他4组与EE组心肌型肌酸激酶(CK-MB)比较差异有统计学意义(P<0.001)。 结论 益智仁具有显著的抗疲劳、抗炎症及对心肌损伤的保护作用。通过抑制力竭运动引起的心肌CK-MB和MYO活性的升高,益智仁降低了心肌损伤的程度。这些发现揭露了益智仁在心脏保护方面的潜在抗氧化作用,并可能成为预防和治疗运动相关心肌损伤的一个重要机制。此外,这些结果为开发心肌损伤干预和治疗药物提供了新的研究思路。 Abstract:Objective To establish a mouse model of exercise-induced myocardial injury caused by exhaustive exercise and to investigate the intervention effect and possible mechanism of Alpiniae oxyphylla fructus pretreatment on myocardial injury after exhaustive exercise by observing the changes of myocardial injury indexes after acute exhaustive exercise in mice, thus providing a theoretical basis and reference for further related studies. Methods Mice were randomly assigned to five different experimental groups by simple random grouping: Control group (Con, n=6), Yizhiren group (Yz, n=6), Exhaustion group (EE, n=6), Exhaustion exercise model + Yizhiren low dose group (EE+EYz, n=6), and Exhaustion exercise model + Yizhiren high dose group (EE+HYz, n=6), all mice received specific exercise intervention and drug feeding for six weeks. The mice were sacrificed at the end of the experiment, and data on biomarkers associated with myocardial injury were collected. Results In terms of exhaustion frequency, EE+HYz group and EE+EYz group showed statistically significant difference compared with EE group (P < 0.05). Statistically significant differences were observed between EE+HYz group, Con and Yz group, EE+HYz and EE group (P < 0.05). In the analysis of biochemical parameters, the level of tumor necrosis factor α (TNF-α) in the EE+HYz group was significantly decreased compared with that in the EE group (P < 0.05). There was significant difference in erythroprotein (MYO) among the 5 groups (P < 0.05). Myocardial creatine kinase (CK-MB) was significantly different between the other 4 groups and EE group (P < 0.001). Conclusions Alpiniae oxyphylla fructus has significant anti-fatigue, anti-inflammation and protective effects on myocardial injury. By inhibiting the increase of myocardial CK-MB and MYO activity induced by exhaustive exercise, Alpiniae oxyphylla fructus reduced the degree of myocardial injury. These findings reveal the potential antioxidant effects of Alpiniae oxyphylla fructus in heart protection and may be an important strategy for the prevention and treatment of exercise-related myocardial injury. In addition, these results provided new research ideas for the development of intervention and treatment drugs for myocardial injury. -
表 1 各组小鼠各项指标比较(x±s)
Table 1. Comparison of various indexes in each group of mice(x±s)
组别 只数 力竭次数(次) 体重(g) TNF-α(pg/mL) MYO(pg/mL) CK-MB(pg/mL) Con 6 26.88±0.31b 0.32±0.04 0.26±0.02 0.25±0.01 Yz 6 25.05±0.73c 0.28±0.02 0.28±0.03 0.25±0.02 EE 6 18±5 25.25±1.36c 0.31±0.06 0.25±0.03 0.32±0.04bc EE+EYz 6 15±6a 24.24±1.45c 0.28±0.04 0.21±0.01abc 0.25±0.01a EE+HYz 6 13±3a 23.70±0.63abc 0.36±0.03ac 0.27±0.03d 0.25±0.01a F值 32.280 8.836 2.133 6.992 17.092 P值 <0.001 <0.001 <0.028 <0.001 <0.001 注:与Con组比较,cP<0.05;与Yz组比较,bP<0.05;与EE组比较,aP<0.05;与EE+EYz组比较,dP<0.05。 -
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