<i>ALDH8A1</i>在胃癌组织中的表达及其临床意义

赵萌; 夏勇生; 王子良; 王俊豪; 葛思堂; 左芦根; 陈德利

doi:10.16766/j.cnki.issn.1674-4152.003582

ALDH8A1在胃癌组织中的表达及其临床意义

doi: 10.16766/j.cnki.issn.1674-4152.003582

赵萌^{1, 2},
夏勇生^{1, 2},
王子良^{1, 2},
王俊豪³,
葛思堂²,
左芦根^{2, 3},
陈德利^2, ,

1.
蚌埠医科大学研究生院，安徽蚌埠 233030
2.
蚌埠医科大学第一附属医院胃肠外科，安徽蚌埠 233004
3.
炎症相关疾病基础与转化研究安徽省重点实验室，安徽蚌埠 233004

基金项目:

安徽省高校自然科学基金重点项目 KJ2021A0685

详细信息

通讯作者:
陈德利，E-mail：13965295950@139.com

中图分类号: R735.2 R730.43
计量
- 文章访问数: 22
- HTML全文浏览量: 8
- PDF下载量: 1
- 被引次数: 0
出版历程
- 收稿日期: 2023-10-24
- 网络出版日期: 2024-09-05

Expression and clinical significance of ALDH8A1 in gastric cancer tissues

ZHAO Meng^{1, 2},
XIA Yongsheng^{1, 2},
WANG Ziliang^{1, 2},
WANG Junhao³,
GE Sitang²,
ZUO Lugen^{2, 3},
CHEN Deli^{2
, ,}

1.
Graduate School of Bengbu Medical University, Bengbu, Anhui 233030, China

摘要

摘要: 目的探究重组表达醛脱氢酶8A1(ALDH8A1)在胃癌组织中的表达情况及与临床病理参数的关系，并分析其对患者远期预后的影响。方法纳入2012年3月—2018年4月在蚌埠医科大学第一附属医院接受根治术治疗的109例胃癌患者，采用免疫组化法分析ALDH8A1在胃癌及癌旁组织的表达情况，并运用统计学方法分析其与临床病理特征的关系；采用Kaplan-Meier曲线分析ALDH8A1的表达对胃癌患者术后5年生存率的影响。结果 ALDH8A1在胃癌组织中的表达明显高于癌旁组织(P＜0.001)。CEA≥5 μg/L、CA19-9≥37 kU/L、癌细胞类型为腺癌、T3~4期及N2~3期患者ALDH8A1表达水平更高(P＜0.05)。Kaplan-Meier曲线分析及多因素分析显示ALDH8A1高表达(HR=2.674，95% CI：1.293~5.533，P=0.008)可能会增加胃癌患者术后的死亡率。ROC曲线表明以2.555为截点值，ALDH8A1对胃癌患者预后具有较高的预判价值(灵敏度为68.97%，特异度为86.27%)。GO和KEGG富集分析显示ALDH8A1与细胞减数分裂、DNA结合和细胞周期等信号通路有关。结论 ALDH8A1在胃癌组织中高表达并促进胃癌的恶性进展，与预后不良相关。
- 胃癌 /
- 重组表达醛脱氢酶8A1 /
- 临床病理特征 /
- 预后
Abstract: Objective To investigate the expression of aldehyde dehydrogenase 8 family member A1 (ALDH8A1) in gastric cancer tissues and its relationship with clinicopathological parameters, and to analyze its effect on the long-term prognosis of patients. Methods A total of 109 patients with gastric cancer who underwent radical resection in the First Affiliated Hospital of Bengbu Medical University from March 2012 to April 2018 were enrolled. Immunohistochemistry was used to analyze the expression of ALDH8A1 in gastric cancer tissues and adjacent tissues, and statistical methods were used to analyze its relationship with clinicopathological features. Kaplan-Meier Plotter curve was used to analyze the effect of ALDH8A1 expression on the 5-year survival rate of patients with gastric cancer after surgery. Results The expression of ALDH8A1 in gastric cancer tissues was significantly higher than that in adjacent tissues (P < 0.001). The high expression rate of ALDH8A1 was higher in patients with CEA≥5 μg/L, CA19-9≥37 kU/L, adenocarcinoma, T3-4 stage and N2-3 stage (P < 0.05). Kaplan-Meier Plotter curve analysis and multivariate analysis showed that high ALDH8A1 expression (HR=2.674, 95% CI: 1.293-5.533, P=0.008) may increase the mortality of patients with gastric cancer after surgery. ROC curve showed that with 2.555 as the cut-off value, ALDH8A1 had a high predictive value for the prognosis of gastric cancer patients (sensitivity was 68.97%, specificity was 86.27%). GO and KEGG enrichment analysis showed that ALDH8A1 was related to cell meiosis, DNA binding and cell cycle signaling pathways. Conclusion ALDH8A1 is highly expressed in gastric cancer tissues and promotes the malignant progression of gastric cancer and is associated with poor prognosis.
- Gastric cancer /
- Aldehyde dehydrogenase 8 family member A1 /
- Clinical pathological features /
- Prognosis

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图 1 ALDH8A1在胃癌及癌旁组织中的表达情况

注：A、B为胃癌及癌旁组织中ALDH8A1表达的HPA数据库分析；C、D为胃癌及癌旁组织中ALDH8A1表达的免疫组织化学染色分析。

Figure 1. of ALDH8A1 in gastric cancer and adjacent tissues

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图 2 ALDH8A1的表达与胃癌患者术后生存率的关系

Figure 2. Association between ALDH8A1 expression and postoperative survival in gastric cancer patients

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图 3 ALDH8A1表达量对胃癌患者术后5年生存率的预测价值

Figure 3. Predictive value of ALDH8A1 expression on 5-year postoperative survival in gastric cancer patients

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图 4 ALDH8A1基因功能富集分析

Figure 4. Functional enrichment analysis of the ALDH8A1 gene

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表 1 胃癌患者ALDH8A1表达水平与临床病理参数的关系[例(%)]

Table 1. Relationship between ALDH8A1 expression level and clinicopathological parameters in gastric cancer patients[cases(%)]

项目	例数	ALDH8A1		χ²值	P值
项目	例数	低表达(n=55)	高表达(n=54)	χ²值	P值
性别				0.074	0.785
男性	78	40(51.28)	38(48.72)
女性	31	15(48.39)	16(51.61)
年龄(岁)				0.860	0.354
＜60	39	22(56.41)	17(43.59)
≥60	70	33(47.14)	37(52.86)
CEA(μg/L)				11.246	0.001
＜5	54	36(66.67)	18(33.33)
≥5	55	19(34.55)	36(65.45)
CA19-9(kU/L)				22.581	< 0.001
＜37	47	36(76.60)	11(23.40)
≥37	62	19(30.65)	43(69.35)
肿瘤直径(cm)				1.614	0.204
＜5	47	27(57.45)	20(42.55)
≥5	62	28(45.16)	34(54.84)
组织学分型				4.975	0.026
腺癌	61	25(40.98)	36(59.02)
其他	48	30(62.50)	18(37.50)
T分期				11.245	0.001
1~2期	58	38(65.52)	20(34.48)
3~4期	51	17(33.33)	34(66.67)
N分期				6.694	0.010
0~1期	54	34(62.96)	20(37.04)
2~3期	55	21(38.18)	34(61.82)

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表 2 胃癌患者术后5年生存率影响因素的单因素及多因素分析

Table 2. Univariate and multivariate analysis of factors influencing the 5-year postoperative survival rate in gastric cancer patients

变量	单因素分析		多因素分析
变量	log-rank χ²	P值	B	SE	Waldχ²	P值	HR值	95% CI
性别	1.406	0.236
年龄	0.615	0.433
ALDH8A1表达	35.350	< 0.001	0.984	0.371	7.034	0.008	2.674	1.293~5.533
CEA	25.434	< 0.001	0.878	0.339	6.727	0.009	2.406	1.239~4.672
CA19-9	31.379	< 0.001	1.314	0.405	10.527	0.001	3.721	1.683~8.231
肿瘤直径	0.928	0.335
组织学分型	2.271	0.132
T分期	22.032	< 0.001	0.693	0.319	4.723	0.030	2.000	1.070~3.737
N分期	17.594	< 0.001	0.941	0.336	7.849	0.005	2.562	1.327~4.946

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