血清sCD14、HMGB1水平与口腔正畸患者发生交叉感染的关系研究

方敏; 邹英; 程春; 王卿; 米丛波

doi:10.16766/j.cnki.issn.1674-4152.003744

血清sCD14、HMGB1水平与口腔正畸患者发生交叉感染的关系研究

doi: 10.16766/j.cnki.issn.1674-4152.003744

新疆医科大学第一附属医院/附属口腔医院口腔正畸科，新疆乌鲁木齐 830000

基金项目:

国家自然科学基金项目 8196030305

详细信息

通讯作者:
米丛波，E-mail：615776249@qq.com

中图分类号: R783.5 R446
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- 文章访问数: 22
- HTML全文浏览量: 10
- PDF下载量: 1
- 被引次数: 0
出版历程
- 收稿日期: 2024-04-11
- 网络出版日期: 2024-12-31

Study of the relationship between serum sCD14, HMGB1 levels and cross-infection in orthodontic patients

Department of Orthodontics, the First Affiliated Hospital of Xinjiang Medical University/Affiliated Stomatological Hospital, Urumqi, Xinjiang 830000, China

摘要

摘要: 目的探究血清可溶性CD14(sCD14)、高迁移率族蛋白B1(HMGB1)水平与口腔正畸患者发生交叉感染的关系及二者对患者发生交叉感染的预测价值。方法以新疆医科大学第一附属医院2018年1月—2022年1月收治的183例口腔正畸患者作为研究对象，其中口腔正畸治疗过程发生交叉感染的患者93例作为感染组，未出现交叉感染的患者90例作为对照组。采用ELISA法检测研究对象血清sCD14、HMGB1水平；多因素logistic回归分析研究口腔正畸患者发生交叉感染的影响因素；ROC曲线分析sCD14、HMGB1与口腔正畸患者发生交叉感染的关系。结果与对照组相比，感染组血清sCD14、HMGB1表达水平显著升高(P＜0.05)；高水平血清sCD14、HMGB1是口腔正畸患者发生交叉感染的独立危险因素；血清sCD14、HMGB1预测口腔正畸患者发生交叉感染的AUC分别为0.769、0.783，两者联合预测口腔正畸患者发生交叉感染的AUC为0.886，均高于sCD14、HMGB1单独检测(P＜0.001)。结论发生交叉感染的口腔正畸患者血清sCD14、HMGB1水平升高，两者联合检测对口腔正畸患者是否发生交叉感染具有一定的预测价值。
- 血清可溶性CD14 /
- 高迁移率族蛋白B1 /
- 口腔正畸 /
- 交叉感染
Abstract: Objective To investigate the relationship between serum soluble cluster of differentiation antigen 14 (sCD14) and high mobility group B1 (HMGB1) levels and the occurrence of cross-infection in orthodontic patients, as well as their predictive value for cross-infection. Methods A total of 183 orthodontic patients admitted to the First Affiliated Hospital of Xinjiang Medical University from January 2018 to January 2022 were taken as the study object. Among them, 93 cases with cross-infection during orthodontic treatment were taken as the infection group, while 90 cases without cross-infection were taken as the control group. The levels of serum sCD14 and HMGB1 were detected by enzyme-linked immunosorbent assay (ELISA). Multivariate logistic regression was used to analyze the influencing factors of cross-infection, and the relationship between sCD14, HMGB1, and cross infection in orthodontic patients was analyzed by ROC curves. Results Compared with the control group, the levels of serum sCD14 and HMGB1 in the infection group were obviously higher (P < 0.05). Elevated levels of serum sCD14 and HMGB1 were independent risk factors for cross-infection in orthodontic patients (P < 0.05). The area under the curve (AUC) for predicting cross-infection was 0.769 for sCD14 and 0.783 for HMGB1. The combined AUC of both markers was 0.886, significantly higher than that for either marker alone (P < 0.001). Conclusion Increased serum levels of sCD14 and HMGB1 are increased with cross-infection in orthodontic patients, and their combined detection offers strong predictive value for the occurrence of cross-infection.
- Serum soluble CD14 /
- High mobility group protein B1 /
- Orthodontics /
- Cross infection

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图 1 血清sCD14、HMGB1预测口腔正畸患者发生交叉感染的ROC曲线

Figure 1. ROC curves of serum sCD14 and HMGB1 for predicting cross-infection in orthodontic patients

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表 1 2组口腔正畸患者基本资料比较

Table 1. Comparison of baseline data between two groups of orthodontic patients

组别	例数	性别[例(%)]		年龄 (x±s，岁)	BMI (x±s)	Fascin蛋白 (x±s，pg/mL)	IGF (x±s，pg/mL)
组别	例数	男性	女性	年龄 (x±s，岁)	BMI (x±s)	Fascin蛋白 (x±s，pg/mL)	IGF (x±s，pg/mL)
对照组	90	43(47.78)	47(52.22)	21.27±6.83	23.65±5.69	6.22±0.74	0.89±0.21
感染组	93	48(51.61)	45(48.39)	20.54±6.51	23.71±6.11	4.17±0.57	0.65±0.16
统计量		0.269^a		0.740^b	0.069^b	21.035^b	8.714^b
P值		0.604		0.460	0.945	＜0.001	＜0.001
注：^a为χ²值，^b为t值。

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表 2 2组口腔正畸患者血清sCD14、HMGB1表达水平比较(x±s)

Table 2. Comparison of serum sCD14 and HMGB1 expression levels between the two groups of orthodontic patients

组别	例数	sCD14(ng/mL)	HMGB1(pg/mL)
对照组	90	1.76±0.53	258.05±76.32
感染组	93	3.02±0.97	421.66±142.51
t值		10.854	9.636
P值		＜0.001	＜0.001

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表 3 口腔正畸患者血清sCD14、HMGB1水平与Fascin蛋白、IGF的相关性分析

Table 3. Correlation analysis of serum sCD14 and HMGB1 levels with Fascin protein and IGF in orthodontic patients

项目	sCD14		HMGB1
项目	r值	P值	r值	P值
Fascin蛋白	-0.454	＜0.001	-0.503	0.021
IGF	-0.475	＜0.001	-0.492	＜0.001

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表 4 口腔正畸患者发生交叉感染影响因素的多因素logistic回归分析

Table 4. Multivariate logistic regression analysis of factors influencing the occurrence of cross-infection in orthodontic patients

变量	B	SE	Waldχ²	P值	OR值	95% CI
sCD14	0.546	0.218	6.269	0.012	1.726	1.126~2.646
HMGB1	0.934	0.342	7.460	0.006	2.545	1.302~4.975
Fascin蛋白	-1.124	0.472	5.670	0.017	0.325	0.129~0.820
IGF	-1.802	0.562	10.279	0.001	0.165	0.055~0.496

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