手足口病对儿童口腔菌群的影响研究

蒋亚洲; 朱苏月; 乔继冰; 沈男; 吴让; 刘峰

doi:10.16766/j.cnki.issn.1674-4152.003841

手足口病对儿童口腔菌群的影响研究

doi: 10.16766/j.cnki.issn.1674-4152.003841

蒋亚洲^{1, 2},
朱苏月²,
乔继冰²,
沈男²,
吴让²,
刘峰^1, ,

1.
南京医科大学附属儿童医院呼吸科, 江苏南京 210008
2.
徐州医科大学附属宿迁医院儿科, 江苏宿迁 223800

基金项目:

江苏省妇幼健康科研项目 F201941

江苏省妇幼健康科研项目 F202153

详细信息

通讯作者:
刘峰, E-mail: axsliu@163.com

中图分类号: R512.5
计量
- 文章访问数: 25
- HTML全文浏览量: 20
- PDF下载量: 1
- 被引次数: 0
出版历程
- 收稿日期: 2024-02-24
- 网络出版日期: 2025-02-13

Effects of hand, foot, and mouth disease on oral microbiota in children

JIANG Yazhou^{1, 2},
ZHU Suyue²,
QIAO Jibing²,
SHEN Nan²,
WU Rang²,
LIU Feng^{1
, ,}

1.
Department of Respiration, Children′ s Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, China

摘要

摘要: 目的利用高通量测序技术对手足口病(HFMD)儿童的口腔菌群进行测定并分析其变化特点。方法选取2021年4月—2023年9月在徐州医科大学附属宿迁医院确诊为HFMD的患儿86例以及同期在院体检健康的儿童38名，分别纳入HFMD组和对照组。收集所有纳入儿童的双侧颊黏膜唾液拭子并从中提取基因组DNA，质检后行16S rDNA扩增子测序。对测序获取的生物学信息进行聚类、注释、组间比较。利用α和β多样性分析分别比较丰度、多样性和结构变化；对2组丰度前十的关键菌属再次进行显著差异比较，筛选生物标记物。结果 2组共聚类操作分类单元(OTU)为1 891个，其中HFMD组1 047个，对照组844个，组间共有544个。相对于健康儿童，HFMD组的口腔菌群结构发生改变，shannon指数(P＜0.05)和ace指数(P＜0.05)均显著降低。关键菌属中以纤毛菌属(P＜0.05)和二氧化碳嗜纤维菌属(P＜0.05)丰度降低为标志特点。结论 HFMD儿童口腔菌群存在紊乱，多样性和丰度显著降低，且以产短链脂肪酸菌属丰度降低为特点。该结果可为后期研究HFMD微生态学机制及其靶向干预提供部分理论依据。
- 手足口病 /
- 口腔菌群 /
- 儿童
Abstract: Objective To detect and analyze the changes in oral microbiota of children with hand, foot, and mouth disease (HFMD) by high-throughput sequencing technology. Methods A total of 86 children diagnosed with HFMD in Suqian Hospital Affiliated to Xuzhou Medical University from April 2021 to September 2023, as well as 38 healthy children who underwent physical examinations during the same period, were selected as the HFMD group and the control group, respectively. Bilateral buccal mucosal saliva swabs of all the included children were collected and genomic DNA was extracted. 16S rDNA amplicon sequencing was performed after quality inspection. The biological information obtained by sequencing was clustered, annotated, and compared between groups. α diversity analysis and β diversity analysis were used to compare the abundance, diversity, and structural changes. The top ten abundant key bacteria genera of the two groups were significantly compared again to screen biomarkers. Results A total of 1 891 OTUs were obtained in the two groups, 1 047 in the HFMD group, and 844 in the control group, with 544 between the groups. Compared to healthy children, the oral microbiota structure of HFMD was changed, and the shannon index (P < 0.05) and ACE index (P < 0.05) were significantly decreased. The key bacterial genera are characterized by a decrease in the abundance of Leptotrichia (P < 0.05) and Capnocytophaga (P < 0.05). Conclusion The oral microbiota of HFMD is disordered, with significantly reduced diversity and richness, characterized by a decrease in short-chain fatty acids-producing bacteria. These results can provide some theoretical basis for the study of HFMD microecology mechanism and its targeted intervention in the future.
- Hand, foot, and mouth disease /
- Oral microbiota /
- Children

HTML全文

图 1 HFMD组和对照组口腔菌群的α多样性分析

注：A为shannon指数；B为ace指数。

Figure 1. Alpha diversity of oral microbiota in the HFMD and control groups

下载: 全尺寸图片幻灯片

图 2 2组受试者口腔菌群在OTU水平上的PLS-DA分析

Figure 2. PLS-DA analysis of oral microbiota at the OTU level in two groups of subjects

下载: 全尺寸图片幻灯片

图 3 HFMD组和对照组口腔菌群中主要菌类的相对丰度比例

注：A为门水平；B为属水平。

Figure 3. The relative abundance of predominant bacteria in the oral microbiota of the HFMD and control groups

下载: 全尺寸图片幻灯片

图 4 HFMD组与对照组中相对丰度前十的菌属比较

Figure 4. Comparison of the relative abundance of the top 10 species at the genus level between the HFMD and control groups

下载: 全尺寸图片幻灯片

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