血清miR-760-3p和miR-182-5p在急性缺血性脑卒中患者中的表达及与预后的关系

段凯强; 吴艳峰; 薛嘉海; 王巍; 李春玲; 李娟

doi:10.16766/j.cnki.issn.1674-4152.004209

血清miR-760-3p和miR-182-5p在急性缺血性脑卒中患者中的表达及与预后的关系

doi: 10.16766/j.cnki.issn.1674-4152.004209

1.
张家口市第一医院急诊科，河北张家口 075000
2.
张家口市第一医院放射医学科
3.
张家口市第一医院神经内三科
4.
张家口市第一医院老年病二科

基金项目:

河北省2022年度医学科学研究项目 20221896

详细信息

通讯作者:
李娟，E-mail：411676641@qq.com

中图分类号: R743.3 R730.7
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出版历程
- 收稿日期: 2025-02-24

The expression of serum miR-760-3p and miR-182-5p in patients with acute ischemic stroke and their relationship with prognosis

1.
Emergency Department, Zhangjiakou First Hospital, Zhangjiakou, Hebei 075000, China

摘要

摘要: 目的通过检测急性缺血性脑卒中(AIS)患者血清miR-760-3p和miR-182-5p表达，探究其与患者病情及预后的关系。方法选取张家口市第一医院2022年2月—2024年2月收治的179例AIS患者为观察组，根据病情严重程度分为轻度组(59例)、中度组(75例)和重度组(45例)，根据患者预后情况分为预后良好组(132例)和预后不良组(47例)，另选同期本院179例体检健康者作为对照组。分析血清miR-760-3p、miR-182-5p与NIHSS评分的相关性，AIS患者预后的影响因素，血清miR-760-3p、miR-182-5p对AIS患者预后的预测价值。结果观察组、预后不良组血清miR-760-3p、miR-182-5p水平分别低于对照组和预后良好组(P＜0.05)。随着病情不断加重，患者血清miR-760-3p、miR-182-5p水平依次降低(P＜0.05)。血清miR-760-3p、miR-182-5p水平与患者NIHSS评分呈负相关关系(r_s=-0.578、-0.618，P < 0.001)。年龄、高血压史、吸烟史和血清miR-760-3p、miR-182-5p水平均为AIS患者预后不良的影响因素(P＜0.05)。两者联合预测AIS患者预后的AUC高于miR-760-3p、miR-182-5p单独预测(P＜0.05)。结论 miR-760-3p和miR-182-5p在AIS患者血清中表达下调，与患者预后密切相关。
- 急性缺血性脑卒中 /
- miR-760-3p /
- miR-182-5p /
- 预后
Abstract: Objective By detecting the expression of serum miR-760-3p and miR-182-5p in patients with acute ischemic stroke (AIS), aimed to further explore their relationship with the patient ' s condition and prognosis. Methods From February 2022 to February 2024, 179 patients with AIS admitted to Zhangjiakou First Hospital were selected as the observation group, and divided into a mild group of 59 cases, a moderate group of 75 cases, and a severe group of 45 cases, and 179 healthy individuals who underwent physical examinations at our hospital were regarded as the control group. According to the patient ' s prognosis, they were divided into a good prognosis group 132 cases and a poor prognosis group 47 cases. The correlation between serum miR-760-3p and miR-182-5p expression levels and NIHSS score, the prognostic factors of patients with AIS, and the predictive value of serum miR-760-3p and miR-182-5p for the prognosis of patients with AIS were analyzed. Results The expression levels of miR-760-3p and miR-182-5p in the observation group and poor prognosis group were lower than those in the control group and good prognosis group, respectively (P < 0.05). As the severity of the disease deepens, the expression levels of miR-760-3p and miR-182-5p in the patient ' s serum decreased sequentially (P < 0.05). The expression levels of serum miR-760-3p and miR-182-5p were negatively correlated with NIHSS scores (r_s=-0.578, -0.618, P < 0.001). The age, smoking history, hypertension history, and serum miR-760-3p and miR-182-5p were influencing factors for poor prognosis in patients with AIS (P < 0.05). ROC curve results showed that the AUC of their combination in predicting the prognosis of AIS patients was higher than that of miR-760-3p and miR-182-5p alone (P < 0.05). Conclusion The downregulation of miR-760-3p and miR-182-5p expression in the serum of AIS patients was closely related to prognosis.
- Acute ischemic stroke /
- miR-760-3p /
- miR-182-5p /
- Prognosis
利益冲突 无

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图 1 血清miR-760-3p、miR-182-5p预测AIS患者预后的ROC曲线

Figure 1. ROC curves of serum miR-760-3p and miR-182-5p for predicting the prognosis of AIS patients

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表 1 AIS患者组与对照组一般资料比较

Table 1. Comparison of general data between the AIS patient group and the control group

组别	例数	性别[例(%)]		年龄(x±s，岁)	BMI(x±s)
组别	例数	男性	女性	年龄(x±s，岁)	BMI(x±s)
观察组	179	92(51.40)	87(48.60)	67.12±8.25	22.38±2.37
对照组	179	95(53.07)	84(46.93)	67.82±8.56	22.54±2.42
统计量		0.101^a		0.788^b	0.632^b
P值		0.751		0.431	0.528
注：^a为χ²值，^b为t值。

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表 2 引物序列

Table 2. Primer sequence

基因	正向引物5’-3’	反向引物5’-3’
miR-760-3p	TCGGCAGGCGGCTCTGGGTCTG	CTCAACTGGTGTCGTGGA
miR-182-5p	TGCGGTTTGGCAATGGTAGAAC	CCAGTGCAGGGTCCGAGGT
U6	CTCGCTTCGGCACCACA	AACGGTTCACGGATTTGCGT

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表 3 2组血清miR-760-3p、miR-182-5p表达水平比较(x±s)

Table 3. Comparison of the expression levels of serum miR-760-3p and miR-182-5p between the two groups(x±s)

组别	例数	miR-760-3p	miR-182-5p
观察组	179	0.74±0.16	0.78±0.17
对照组	179	1.06±0.05	1.03±0.06
t值		25.540	18.553
P值		< 0.001	< 0.001

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表 4 不同神经损缺严重程度AIS患者血清miR-760-3p、miR-182-5p表达水平和NIHSS评分比较(x±s)

Table 4. Comparison of serum miR-760-3p and miR-182-5p expression levels and NIHSS scores among AIS patients with differing severities of neurological impairment(x±s)

组别	例数	miR-760-3p	miR-182-5p	NIHSS评分(分)
轻度组	59	0.91±0.08	0.94±0.06	3.19±0.92
中度组	75	0.72±0.13^a	0.75±0.17^a	12.46±2.38^a
重度组	45	0.56±0.15^ab	0.61±0.15^ab	17.94±2.69^ab
F值		107.551	75.866	661.997
P值		< 0.001	< 0.001	< 0.001
注：与轻度组比较，^aP＜0.05；与中度组比较，^bP＜0.05。

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表 5 预后良好组和预后不良组AIS患者一般资料比较

Table 5. Comparison of baseline characteristics between AIS patients with good and poor prognosis

项目	预后良好组(n=132)	预后不良组(n=47)	统计量	P值
性别[例(%)]			0.934^a	0.334
男性	65(49.24)	27(57.45)
女性	67(50.76)	20(42.55)
年龄(x±s，岁)	66.39±7.59	69.17±8.12	2.117^b	0.036
BMI(x±s)	22.29±2.31	22.65±2.36	0.912^b	0.363
吸烟史[例(%)]			5.165^a	0.023
有	56(42.42)	29(61.70)
无	76(57.58)	18(39.30)
饮酒史[例(%)]			0.967^a	0.325
有	70(53.03)	21(44.68)
无	62(46.97)	26(55.32)
高血压史[例(%)]			7.586^a	0.006
有	59(44.70)	32(68.09)
无	73(55.30)	15(31.91)
糖尿病史[例(%)]			1.449^a	0.229
有	68(51.52)	29(61.70)
无	64(48.48)	18(38.30)
高脂血症[例(%)]			0.799^a	0.371
有	63(47.73)	26(55.32)
无	69(52.27)	21(44.68)
NIHSS评分(x±s，分)	8.85±1.36	16.21±2.89	23.375^b	< 0.001
治疗措施[例(%)]			2.765^a	0.096
静脉溶栓	72(54.55)	19(40.43)
血管内治疗	60(45.45)	28(59.57)
注：^a为χ²值，^b为t值。

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表 6 预后良好组和预后不良组AIS患者血清miR-760-3p、miR-182-5p表达水平比较(x±s)

Table 6. Comparison of serum miR-760-3p and miR-182-5p expression levels between AIS patients between AIS patients with good and poor prognosis(x±s)

组别	例数	miR-760-3p	miR-182-5p
预后良好组	132	0.81±0.18	0.84±0.19
预后不良组	47	0.56±0.12	0.62±0.15
t值		8.840	7.177
P值		< 0.001	< 0.001

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表 7 变量赋值情况

Table 7. Variable assignment

变量	赋值方法
AIS患者预后是否良好	是=0，否=1
年龄	连续变量，以实际值赋值
吸烟史	有=1，无=0
高血压史	有=1，无=0
血清miR-760-3p表达水平	连续变量，以实际值赋值
血清miR-182-5p表达水平	连续变量，以实际值赋值

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表 8 AIS患者预后的影响因素分析

Table 8. Analysis of factors influencing prognosis of AIS patients

变量	B	SE	Waldχ²	P值	OR值	95% CI
年龄	0.618	0.249	6.168	0.013	1.856	1.139~3.024
吸烟史	1.324	0.496	7.124	0.008	3.758	1.422~9.935
高血压史	1.018	0.432	5.558	0.018	2.769	1.187~6.457
miR-760-3p表达水平	-0.246	0.115	4.572	0.032	0.782	0.624~0.980
miR-182-5p表达水平	-0.622	0.265	5.505	0.019	0.537	0.319~0.903

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