Abstract: Objective To evaluate the cerebrovascular reactivity of patients with glioma by breath-hold fMRI and plan the safest surgical trajectory. Methods Eight patients with glioma (five male and three female;ages 18 to 53 years old,average 33.4 years) were performed the fMRI with resting-state and breath holding task.SPM8 was applied in data processing(P＜0.05，FDR correction),AFNI was used to calculate the percentage of signal change of BOLD in the tumor regional and contralateral normal brain tissue.The difference of the signal change in two ROI was analyzed.The resting-state fMRI(R-fMRI) mapping and BH fMRI CVR mapping was compared. Results Independent component analysis(ICA) was not only able to reveal the location of the motor function area,but also can display the different functional systems around the tumor tissue.Relative to the mirror side normal brain parenchyma,BH BOLD signal around glioma was significantly decreased(P＜0.05),and there was the intersection of the negative region of BOLD signal in R-fMRI. Conclusion ICA of R-fMRI was more comprehensive and accurate in the function system of tumor tissue.BH-fMRI was a convenient and reliable method for CVR assessing to suggest the phenomenon of NVU,and to find false negative areas of fMRI function mapping.It could provide important reference for the formulation of brain tumor surgical plan and the judgment of risk after operation.