Abstract: Objective To investigate the relationship between the serum testosterone levels and age with the risk of metabolic syndrome. Methods A total of 485 female aged 40-65 years from Gongshu District Family Planning Publicity and Technical Guidance Station in Hangzhou City from January to June in 2014 were enrolled into this study.Testosterone levels were determined by automated chemiluminescent method.According to testosterone levels,they were divided into 4 groups,group A(0-0.40 nmol/L,n=158),group B(＞0.40-0.60 nmol/L,n=106) and group C(＞0.60-0.90 nmol/L,n=102) and group D(＞0.90-2.80 nmol/L,n=119).The correlation of serum testosterone with metabolic syndrome was analyzed by multivariate Logistic regression analysis using SPSS 16.0. Results Of 485 women,105 cases were with metabolic syndrome,the total rate of metabolic syndrome was 21.6%.The rate of metabolic syndrome in groups A,B,C and D was 29.1%,23.6%,12.7% and 17.6%,respectively.The rate of metabolic syndrome in serum testosterone levels in women was significant differences(χ²=11.316,P=0.010).Logistic regression analysis showed that,after adjusting for age,the risk of metabolic syndrome declined with serum testosterone levels rising(OR=0.780,95%CI:0.642-0.948). Conclusion Low levels of testosterone may be a potential risk factors for metabolic syndrome.