Abstract: Objective To observe and explore the influence and significance of Hepatic Transcatheter Arterial Chemoembolization(TACE) combine with Endostar on serum VEGF,hypoxia inducible factor(HIF)-1,osteopontin(OPN),matrix metalloproteinase 9(MMP-9) in patients with Hepatocellular carcinoma(HCC). Methods Total 80 cases of HCC between July 2011 and November 2014 in our hospital were selected and divided into two groups according to the random number method.The control group received TACE treatment,while the observation group receiged the combined therpaudy with endostar and TACE.HIF-1A,OPN,VEGF and MMP-9 levels were measured before the treatment and 4 weeks after the treatment,the efficacy in both groups were evaluated after the treatment,and adverse reactions were recorded and compared. Results The difference of VEGF,HIF-1A,OPN and MMP-9 levels before the treatment was not statistically significant(P>0.05),after the treatment,VEGF,HIF-1A,OPN and MMP-9 levels in the observation group were(431.2±132.2)pg/ml,(58.4±0.7)ng/L,(76.4±18.2)μg/L and(194.3±82.2)ng/ml,respectively,while was(562.8±184.4)pg/ml,(62.4±1.1)ng/L,(88.3±19.2)μg/L and(238.4±94.4)ng/ml,respectively,in the control group,VEGF,HIF-1A,OPN and MMP-9 levels in the observation group were significantly lower than those in the control group(P<0.05);The efficacy in observation group was 95.0%(38/40),while was 77.5%(31/40)in the control group,the observation group was significantly higher(P<0.05);the difference of adverse reactions incidence was not statistically significant(P>0.05). Conclusion Endostar combined with TACE in the treatment of HCC can reduce VEGF,HIF-1A,OPN and MMP-9 levels,and limit tumor progression effect.