Abstract: Objective To observe the effect of evidence-based nursing in ptients with painless gastroscopy,and compare the nursing satisfaction of patients receiving evidence-based nursing or routine nursing. Methods Total 100 patients undergoing painless gastroscopy in our hospital from July 2014 to June 2015 were selected and divided into research group and control group with 50 cases in each group.The general information of two groups had no statistical significance difference,it has comparability.The research group received the evidence-based nursing while the control group receieved the routine nursing.All the patients took Self-Rating Anxiety Scale(SAS)before and after the nursing to evaluate their anxiety condition,took Newcastle Satisfaction of Nursing Service(NSNS)research after the examination to evaluate their satisfaction of nursing service.SPSS 19.0 statistical software was used to analyze data.The measurement data was expressed by x±s,and adopted t-test.The categorical data adopted Chi-square test.P<0.05 expressed that difference has statistical significance. Results Before the nursing,the SAS of research group was(47.57±10.22).After the nursing,the SAS of the research group was(40.13±6.07).The anxiety condition of research group had an obvious improvement(t=4.43,P<0.05).Before the nursing,the SAS of control group was(47.74±11.36).After the nursing,the SAS of control group was(46.58±7.53).The anxiety condition of control group had an unconspicuous improvement(t=0.60,P>0.05).The NSNS of research group was(73.85±1.57).The NSNS of research group was(67.33±2.74).The nursing satisfaction of research group was obviously higher than control group(t=14.60,P<0.05). Conclusion The inspection time for painless gastroscopy is short and success rate is high.Evidence-based nursing can scientifically combine clinical knowledge and experience.Evidence-based nursing for painless gastroscopy can relieve the pain of patients.Evidence-based nursing can obviously relieve the anxiety degree of patients with painless gastroscopy.It’s contributed to improve nursing satisfaction and the trust of doctor-patient,and reduce medical dispute.