Abstract: Objective To analyse the expression of cytokines in serum from patients who are in the different periods of acute cerebral infarction by the cytokines antibody chip technology. Methods Fourty cases of acute cerebral infarction patients were collected,according to different onset time,divided into five groups,2 hours,4 hours,6 hours,24 hours and 72 hours,8 cases in each group.In addition, 8 healthy cases were used as controls.Apply cytokines antibody chip technology to detect 60 kinds of cytokines in serum. Results Compared with healthy controls,the expression of cytokines in blood spectrum of cerebral infarction patients has changed significantly.①There are 15 kinds of cytokines generally appears as a high level,including:IL-1β,IL-1ra,IL-2,IL-5,IL-6,IL-7,IL-10,IL-15,IL-16,GM-CSF,M-CSF,TNF-α,LIGHT,GDNF,Leptin and so on.Among them,some are higher in the early cerebral infarction incidence,including:IL-6,IL-7,IL-10.Otherwise some express more in late than early,including:IL-5，GM-CSF.Some are particularly high in one specific period of cerebral infarction,including:IL-5,IL-3,IL-15,MCP-4.② There are 9 kinds of cytokines expressing lower level,including:BMP-4,Eotaxin-3,IGFBP-3,IGFBP-4,GCP-2,I-309,MCP-1,RANTES,CNTF. Conclusion The cytokines may directly or indirectly involve in the activation and infiltration of the inflammatory cells,and play an important role in the process of the damaging and repairing the neurons.