Abstract: Objective To explore the correlation of cervical secretion fFN level and serum G-CSF level withchorioamnionitis in patients with premature rupture of membranes(PROM). Methods Total 107 patients with premature rupture of membranes were selected as pathology objects and divided into 3 groups according to the occurrence and degree of chorioamnionitis: the patients without chorioamnionitisinto Group A,the patients with subclinical chorioamnionitisinto Group B,and the patients with clinical chorioamnionitisinto Group C.Thirty-three normal pregnancy were recruited as control group.The peripheral venous blood and cervical secretions were prepared before the deliver,and the placenta tissue were extracted after the deliver for the test of fFN and G-CSF. Results The cervical secretion fFN level and placental tissue fFN level of Group A,B,C and control group were as follow: group C>B>A>control(P<0.05).There was a positive correlation between cervical secretion fFN level and placental tissue fFN level(R²=0.521,P<0.001) in patients with premature rupture of membranes.The serum G-CSF level and placental tissue G-CSF level of Group A,B,C and control group were as follow: group C>B>A> control(P<0.05).There was positive correlation between serum G-CSF level and placental tissue G-CSF level(R²=0.865,P<0.001).Both the cervical secretion fFN and serumG-CSF level had diagnostic value to clinical chorioamnionitis and subclinical chorioamnionitis(P<0.05). Conclusion Both cervical secretion fFN and serum G-CSF havea predictive value on subclinical chorioamnionitis,and its clinical survey has positive significance for the prevention and cure of clinical chorioamnionitis.