Abstract: Objective To explore the relationship between amplitude-integrated electroencephalogram(aEEG) and umbilical arterial blood gas analysis,Apgar score of neonatal hypoxic-ischemic encephalopathy(HIE),and to find an effective method for early diagnosis of neonatal HIE. Methods According to the results of aEEG test,97 neonates with HIE were divided into aEEG normal group,aEEG mild group and aEEG severe group.The aEEG,umbilical arterial blood gas analysis and Apgar score were performed in all the three groups.The relationship between aEEG and neonatal HIE clinical graduation,MRI graduation,umbilical arterial blood gas analysis,Apgar score of neonatal HIE were assessed. Results The differences of HIE clinical grading among the three groups were statistically significant (H=6.598,P<0.05);Spearman rank correlation analysis showed that the results of aEEG examination and the clinical index of neonatal HIE were statistically correlated(r=0.758,P<0.05).The differences of MRI grading among the three groups were statistically significant(H=8.415,P<0.05);Spearman rank correlation analysis showed that the results of aEEG examination and MRI grading were statistically correlated(r=0.710,P<0.05).One-way ANOVA showed that the differences of blood gas pH and blood gas BE in the three groups were statistically significant(P<0.05);the differences of 1 min,5 min,10 min Apgar scores among the three groups were statistically significant(P<0.05). Conclusion The aEEG examination is an effective method for early diagnosis of neonatal HIE,and has good clinical application value.