Abstract: Objective To investigate the feasibility of using blood samples from internal jugular vein by vein catheter instead of peripheral vein on maintained hemodialysis patients with permanent central vein catheter when monitoring the coagulation time on hemodialysis patients taking warfarin. Methods Thirty maintained hemodialysis patients with permanent central vein catheter taking warfarin recruited in this study.Get the blood samples from the same patient at the same time by three different methods:routine blood specimens from peripheral vein as a control;20 ml blood specimens from permanent catheter in jugular vein(method one,group one);40 ml blood specimens from permanent catheter in jugular vein(method two,group two).Compares the coagulation time among those groups. Results Compared to the control group,the prothrombin time (PT) and international normalized ratio (INR),activated partial thromboplastin time (APTT),were of significant difference in group one patient(P＜0.01).The levels of PT and INR were the same in control group and group two (P＞0.05),however,APTT level was different in those two group(P＜0.05). Conclusion Blood specimens from peripheral vein is preferred when monitoring the coagulation time on hemodialysis patients taking warfarin.Methods two can be used as a substitute on patients with difficulties of blood drawing under the guidance and interpretation of doctors.