Abstract: Objective To observe the effect of racemic hyoscyamine eye drops applied in juvenile myopia. Methods One hundred and sixty-six cases of adolescent patients with 332 myopia eyes treated in department of ophthalmology of Lishui central hospital from December 2012 to March 2014 were selected and divided into group A and groupB,randomly.0.05% racemic hyoscyamine was given in group A other than wearing glasses,and only wearing glassesgiven in group B.Visual acuity and adverse reaction were compared in 1 year. Results The mean vision in group A was 5.01±0.03，while 4.82±0.09 in group B.It was higher in group A than that in group B(t=10.504，P<0.05).The change of diopter in group A was(0.07±0.02)D,while (0.49±0.11)D in group B.It was lower in group A than that in group B(t=18.051，P<0.05).The change of axial in group A was (0.09±0.04) mm,while (0.14±0.04) mm in group B,it was lower in group A than that in group B(t=3.647，P<0.05).The intraocular pressure in group A was(15.01±2.30) mm Hg,while (14.97±2.11) mm Hg in group B.There was no statistical differences between the two groups(t=1.181，P>0.05).There were 77 cases of vision improvement,117 cases of visual disability,and 138 cases of vision decreasing in group A,and the vision decreasing rate was 41.57%，There were 24 cases of vision improvement,57 cases of visual disability,and 251 cases of vision decreasing in group A,and the vision decreasing rate was 75.60%.The decreasing rate in group A was lower than that in group B(χ²=19.015，P<0.05).There was no symptoms such as photophobia,blurred vision,eye pain,eye itching etc.and there was no obvious exception under slit lamp examination. Conclusion It was effective applying 0.05% racemic hyoscyamine treating juvenile myopia,and safe.It was worthy of clinical application.