Abstract: Objective To analyze the clinical features of Mycoplasma Pneumoniae pneumonia(MPP) complicated with Epstein-Barr virus(EBV) infection in children. Methods Total 94 cases of MPP children in our hospital from August,2013 to December,2014 were enrolled. Forty-one cases were diagnosed of MPP complicated with EBV infection(research group),53 cases of MPP without EBV infection were as control. The general information,clinical manifestation,auxiliary examination,extra-pulmonary complications,therapeutic method and prognosis were analyzed. Results The constituent ratio of age in both groups had statistically significant difference(P < 0. 05). Length of hospital stay,duration of pulmonary rales,severe pneumonia occurrence rates(29. 3%,5. 7%) and lymphadenectasis occurrence rates(41. 4%,15. 1%) of the two groups had significant differences(P < 0. 01). The occurrence rates of elevated white blood cell amount(36. 6%,13. 2%),pleural effusion(9. 8%,0. 0%) and pulmonary consolidation(2. 4%,0. 0%) had significant differences(P <0. 01). The occurrence rates of liver function damage(9. 8%,0. 0%) had significant difference(P < 0. 05). No significant difference was found between two groups in febrile time,occurrence rates of elevated C-reactive protein,erythrocyte sedimentation rate,procalcitonin,and extra-pulmonary complications(P > 0. 05). Conclusion The infants with MPP are more likely to be complicated with EBV infection. Compared with MPP cases,MPP complicated with EBV infection cases have longer duration of pulmonary rales and higher occurrence of pleural effusion,pulmonary consolidation,liver function damage,lymphadenectasis,severe pneumonia and longer length of hospital stay. In some cases the adoption of glucocorticoid or protopic immunomodulator may be necessary.