Abstract: Objective To check the expression and location of ezrin,microfibril and microtubule in endometrium tissues of patients with endometriosis,and explore the subcellular mechanisms of endometriosis. Methods The expression and location of ezrin,microfibril and microtubule in the endometrial cells of 13 women with normal endometrium,and in the eutopic and ectopic endometrium cells of 11 patients with endometriosis were examined by immunofluorescence(fluorescence intensity semiquantitative test) and laser confocal microscope,t test was used to analyze the difference between groups by using SPSS 11. 5. Results Ezrin was dispersedly distributed in stroma of ectopic endometrium and the cells lost their normal polarity; The level of Ezrin protein in ectopic endometrial stroma(47. 88 ±6. 75) was significantly higher than in normal endometrium from healthy controls(8. 64 ±1. 61) and in the homologous eutopic endometrium(7. 97 ±0. 93),P < 0. 05; Microfibril was dispersedly distributed both in stroma and glandular epithelium cells of ectopic endometrium tissue; the expression level of microfibril in ectopic endometrial stroma(71. 36 ±4. 27) was ignificantly higher than homologous eutopic endometrium(35. 98 ±5. 76) and normal endometrium from healthy controls(40. 68 ±6. 12),P <0. 05; while the expression levels in the glandular epithelium cells of ectopic,homologous eutopic and normal endometrium were 21. 74 ±2. 28,37. 99 ±6. 60 and 41. 54 ±2. 14,respectively,P < 0. 05. There was no significant difference in the expression of microtubule among the three groups,P > 0. 05. Conclusion The cell migration and implantation in the endometriosis are accompanied with cytoskeleton reconstructions; The expression of ezrin is involved in the occurrence of endometriosis and cytoskeleton reconstructions of endometriosis; Stroma cells play an important role in the process of cell migration and invasion in endometriosis.