Abstract: Objective To explore the clinical significance of serum tumor maker lens culinaris agglutinin-reactive α-fetoprotein(AFP-L3) and Golgi protein-73(GP73) single or combined detection in the diagnosis of primary hepatic carcinoma(PHC). Methods The serum levels of AFP-L3 and GP73 in 80 patients with PHC,in 50 patients with cirrhosis of liver and in 50 healthy cases was detected by the enzyme-link immunosorbent assay (ELISA),and were compared among difference groups,and the clinical diagnosis value of AFP-L3 and GP73 in PHC was evaluated. Results The levels of serum AFP-L3 in patients with PHC,cirrhosis and healthy group:(65.234±21.315)ng/ml,(18.614±20.655)ng/ml and (13.209±14.560)ng/ml,respectively.The serum AFP-L3 in patients with PHC were higher than that in the cirrhosis of liver group and in the healthy cases group(P<0.001).Taking AFP-L3 level ≥ 36.076 ng/ml as diagnostic criteria,the sensitivity of AFP-L3 level in PHC diagnosis was 75.00% and the specificity was 94.00%.The levels of serum GP73 in the patients with PHC,patients with cirrhosis of liver and healthy group were (0.431±0.180)IU/ml,(0.228±0.047)IU/ml and (0.213±0.034)IU/ml,respectively.The serum GP73 in patients with PHC were higher than that in the cirrhosis of liver group and in the healthy cases group(P<0.001).Taking GP73 level ≥ 0.298 IU/ml as diagnostic criteria,the sensitivity of GP73 level in PHC diagnosis was 80.00%,the specificity of GP73 level in PHC diagnosis of GP73 level in PHC diagnosis was 96.00%.The combination of AFP-L3 and GP73 was sensitivity of 92.50% and specificity of 90.00%. Conclusion AFP-L3 and GP73 were very effective serum tumor maker in the clinical diagnosis of the PHC.AFP-L3 and GP73 have a higher specificity.AFP-L3 and GP73 combined detection could increase the sensitivity in the PHC clinical diagnosis.AFP-L3 and GP73 combined detection of PHC is useful.