高效液相色谱法测定补骨脂中新补骨脂异黄酮含量

周焕; 耿祥艳; 刘清; 马涛; 方丹君

doi:10.16766/j.cnki.issn.1674-4152.2016.11.004

高效液相色谱法测定补骨脂中新补骨脂异黄酮含量

doi: 10.16766/j.cnki.issn.1674-4152.2016.11.004

周焕^1,2,
耿祥艳^2,3,
刘清^2,3,
马涛^2,3,
方丹君^1, ,

1. 南京医科大学药学院, 江苏南京 211166;
2. 蚌埠医学院第一附属医院国家药物临床试验机构, 安徽蚌埠 233004;
3. 蚌埠医学院药学院, 安徽蚌埠 233000

基金项目:

安徽省高校自然科学研究重点项目(KJ2015A273)

详细信息

通讯作者:
方丹君,E-mail:fangdanjun@hotmail.com

中图分类号: R917;R282.71
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出版历程
- 收稿日期: 2016-05-20

Determination of neobavaisoflavone in Psoralea corylifolia L. by HPLC

ZHOU Huan^1,2,
GENG Xiang-yan^2,3,
LIU Qing^2,3,
MA Tao^2,3,
FANG Dan-jun^{1
, ,}

School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu 211166, China

摘要

摘要: 目的用高效液相色谱法(high performance liquid chromatography，HPLC)建立补骨脂中新补骨脂异黄酮的含量测定方法。方法使用色谱柱WondaSil^® C₁₈(150.0 mm×4.6 mm，5 μm)，以0.05%三氟乙酸水溶液为流动相A，以乙腈为流动相B，采用梯度洗脱HPLC法，流速为1.0 ml/min，检测波长为254 nm，柱温为30℃，进样量为20 μl。100%甲醇超声30 min提取新补骨脂异黄酮。同种浓度的对照品溶液重复进样6次测定对照品的精密度。平行制备6份新补骨脂异黄酮样品溶液以检测样品溶液的重复性。同一新补骨脂异黄酮样品溶液不同时间段进样5次，测样品溶液的稳定性。平行制备6份样品溶液加入等量的对照品计算加样回收率。结果通过优化色谱条件，建立了中药补骨脂中新补骨脂异黄酮含量的测定方法，考察了专属性、标准曲线、检测限与定量限、精密度、重复性、稳定性、加样回收率。通过方法学考察，新补骨脂异黄酮的线性回归方程为:A=79 375C-9 063.7，R²=0.999，在0.75～12.00 μg/ml的浓度范围内，新补骨脂异黄酮的浓度与峰面积呈良好的线性关系。样品平均加样回收率为97.51%，RSD=1.21%(n=6)。精密度、重复性、稳定性及回收率均良好。不同来源的补骨脂药材中新补骨脂异黄酮的含量差异有统计学意义。结论结果表明所建立的补骨脂中新补骨脂异黄酮的HPLC检测方法操作简便、准确可靠，具有较好的重复性与稳定性，适用于补骨脂的质量控制。
- 补骨脂 /
- 新补骨脂异黄酮 /
- 高效液相色谱 /
- 含量测定
Abstract: Objective To establish a high performance liquid chromatography (HPLC) method for the content determination of neobavaisoflavone in Psoralea corylifolia L. Select the suitable extraction solvent,suitable mobile phase, appropriate determination wavelength and determine the optimal ultrasonic extraction time extraction of Psoralea corylifolia L. of neobavaisoflavone. Methods HPLC was applied and the determination was performed on WondaSil^® C₁₈(150.0 mm×4.6 mm,5 μm).0.05% trifluoroacetic acid aqueous solution as the mobile phase A,and the acetonitrile as mobile phase B,gradient elution HPLC method was applied,solution at a flow rate of 1.0 ml/min and detection wavelength at 254 nm.The column temperature was 30℃,and injection volume was 20 μl.100% 30 min methanol ultrasonic extraction of neobavaisoflavone.The precision of the control sample was repeated 6 times with the same concentration of the control sample.Parallel system repeatability 6 prepared neobavaisoflavone sample solution into the sample solution.The stability of the same neobavaisoflavone sample solution was detemined at different time sampling 5 times the sample solution.Parallel preparation of 6 samples of the sample solution to add the same amount of control samples calculated sample recovery rate. Results Through methodological study, the linear regression equation of neobavaisoflavone is:A=79 375C-9 063.7,R²=0.999,the linear range of Neobavaisoflavone was 0.75-12.00 μg/ml,neobavaisoflavone concentration and peak area showed a good linear relationship.Sample average recovery rate was 97.51%,RSD=1.21%(n=6).Precision,repeatability,stability and recovery were all good.There were differences in neobavaisoflavone different sources of Psoralea corylifolia L. Conclusion By optimizing the chromatographic condition,this paper establishes the method for the determination of Psoralea corylifolia L. in neobavaisoflavone content, examines the special attributes,standard curve,limit of detection and quantitative limit,precision,repetition,stability,and recovery rate.The results showed that the HPLC method for the content determination of neobavaisoflavone in Psoralea corylifolia Lis user-friendly,accurate and reliable, with good repeatability and stability,which can be used for quality control of Psoralea corylifolia L.
- Psoralea corylifolia L /
- Neobavaisoflavone /
- HPLC /
- content determination