Abstract: Objective To observe influence of herbal cake-separated moxibustion and moxibustion on the immunoglobulin levels in immunosuppressive rabbits,and discuss the related mechanism. Methods A total of 40 rabbits were randomly divided into 4 groups:blank group,model group,herbal cake-separated moxibustion group and moxibustion group with 10 rabbits in each group.An immunosuppressive animal model induced by cyclophosphamide(Cy)in rabbit(intraperitoneal injection 60 mg/kg for 7 consecutive days).Two days after the completion of the model,the treatment groups received herbal cake-separated moxibustion and moxibustion(total 10 times,qod.),respectively.The abdominal vein blood was collected after the treatment was finished,and the levels of IgG,IgM,C3 and C4 were tested. Results Compared with the blank group,the levels of IgG,IgM and C3 of model group were significantly lower(P<0.01).After the treatment,The level of IgG changed obviously,and the difference was significant as compared with blank group and model group(P<0.01).The changes of IgM and C3 level were also significant(P<0.01)as compared with model group.For moxibustion group,the levels of IgG,IgM and C3 changed significantly as compared with model group(P<0.01),the level of C3 was lower than blank group(P<0.01). Conclusion Different moxibustion have obvious influence on improving immunosuppressive state by cyclophosphamide in rabbits.The effect of Herbal cake-separated moxibustion is better than moxibustion,which indicates a synergistic effect between herbs and moxibustion.