Various methods of joint inspection for clinical research of idiopathic thrombocytopenic purpura in children
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摘要: 目的 研究儿童特发性血小板减少性紫癜(ITP)网织红细胞及淋巴细胞亚群数值变化的临床意义。 方法 2014年2月—2015年10月在徐州市儿童医院选取ITP患儿110例,根据治疗效果,将110例ITP患儿分为治愈组、有效组、无效组,同时设立对照组(25例)。应用FACS Canto II流式细胞技术仪和Sysmex-XT4000i全自动血细胞分析仪检测各组淋巴细胞亚群的表达及不同荧光强度的网织红细胞(RET)百分比的变化。 结果 ①治愈组、无效组和有效组中T淋巴细胞CD3+、辅助T淋巴细胞Th (CD3+CD4+)显著降低,抑制T淋巴细胞Ts (CD3+CD8+)及B淋巴细胞(CD3+CD19+)显著增高,患者治愈组、无效组和有效组中CD3+、Th、Ts及B淋巴细胞的表达水平与对照组比较,差异均有统计学意义(P<0.05),但各组NK细胞(CD3-CD56+/CD16+)在治疗前后无明显变化,患者组与对照组NK细胞水平的差异无统计学意义(P>0.05);②儿童ITP患者低荧光网织红细胞比率(LFR%)明显降低,而网织红细胞百分比(RET%)、高荧光网织红细胞比率(HFR%)、中荧光网织红细胞比率(MFR%)以及未成熟网织红细胞指数(IRF%)均显著升高,与对照组检测结果比较差异均有统计学意义(P<0.01)。 结论 检测网织红细胞相关参数和T淋巴细胞亚群水平对儿童ITP疾病的诊断及预后判断均具有重要临床价值。
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关键词:
- 网织红细胞 /
- 淋巴细胞亚群 /
- 特发性血小板减少性紫癜 /
- 儿童
Abstract: Objective To study the clinical significance of the changes of reticulocyte(RET)and lymphocyte subsets count in children with idiopathic thrombocytopenic purpura(ITP). Methods Total 110 cases of ITP in our hospital between February,2014 and October,2015 were enrolled and divided into cured group,valid group and invalid group according to the curative effect,and 25 children were selected as control group.FACS Canto II flow cytometry and Sysmex-XT4000i automatic blood cell analyzer were used to test the expression of lymphocyte subgroup and percentage of reticulocyte with fluorescence intensity. Results ①After the treatment,the levels of CD3+ T lymphocytes and (CD3+CD4+) T helper(Th) lymphocytes decreased significantly,inversely,(CD3+CD8+) T suppressor(Ts) lymphocyte and (CD3+,CD19+) B lymphocyte increased obviously.There were significant difference in the expression of CD3+,Th,Ts and B lymphocytes between patients and control(P<0.05),but the expression of NK cells (CD3-CD56+/CD16+) were not improved in each group,and the difference among the all groups was not significant(P>0.05);②In the patients,LFR% decreased obviously,but RET%,HFR%,MFR% and IRF were significantly elevated,and the difference was significant when compared with the control group(P<0.01). Conclusion The reticulocyte related parameters and the level of T lymphocyte subsets of children with ITP play an important role in the diagnosis and prognosis judgment.-
Key words:
- Reticulocyte /
- Lymphocyte subsets /
- Idiopathic thrombocytopenic purpura /
- Children
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