Abstract: Objective To detect the changes of intracranial pressure and the level of S100B and IL-6 in the serum of the patients with severe brain injury after injury,and to analyze the relationship between the changes of serum S100B and IL-6 levels and intracranial pressure. Methods A total of 88 patients with severe brain injury in our hospital from January,2012 to April,2016 were selected as the research subjects.The intracranial pressure of patients was monitored continuously.The venous blood of patients was prepared at 6,12,24,48 and 72 h after the admission.The serum S100B protein and IL-6 levels at different time points were tested by enzyme-linked immunosorbent assay.The relationship between the changes of serum S100B and IL-6 levels and intracranial pressure was analyzed statistically.The t test was performed to compare the means of two groups.Spearman correlation analysis was performed for pairs of quantitative data.When P<0.05,the difference was statistically significant. Results The level of S100B in patients with severe craniocerebral injury increased gradually after the operation,and reached the peak within 24 hours,then decreased gradually;while patients with IL-6 and intracranial pressure after injury gradually increased,serum IL-6 and S-100B levels were significantly associated with intracranial pressure (r=1.933,r=4.859,P<0.05).But the peak value of S100B appeared earlier than that of intracranial pressure;however,the trend of IL-6 was consistent with the change of intracranial pressure. Conclusion The changes of intracranial pressure in patients with severe head injury is proportional to the serum S100B and IL-6 levels.Thus S100B and IL-6 levels can reflect the degree of injury and provide clinical basis for evaluation of patients.