Role of hydrogen sulfide in endotoxinemia and inflammation in rats with intestinal ischemic-reperfusion injury
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摘要: 目的 研究硫化氢对肠缺血-再灌注损伤大鼠血C反应蛋白、降钙素原、内毒素、肿瘤坏死因子-α、白介素-6、白介素-8的影响。 方法 将64只雄性Wistar大鼠随机分为A(假手术)组、B(缺血-再灌注)组,C(缺血-再灌注+NaHS)组,D[缺血-再灌注+炔丙基甘氨酸(PPG)]组。经腹正中切口剖腹及游离肠系膜上动脉(SMA),钳夹B、C、D组大鼠SMA 60 min和再灌注120 min,建立大鼠肠缺血-再灌注损伤模型。C组在灌注前10 min向大鼠尾静脉注入100 μmol/kg NaHS后按1 mg/(kg·h)输注直到再灌注2 h,D组在缺血前10 min向腹腔注入PPG 50 mg/kg。检测血CRP、降钙素原、内毒素、H2S、TNF-α、IL-6、IL-8并进行血培养,测定回肠组织匀浆中H2S。 结果 与A组比较,B组的CRP、降钙素原、内毒素、TNF-α、IL-6、IL-8升高,H2S降低。与B组比较,C组的CRP、降钙素原、内毒素、TNF-α、IL-6、IL-8降低,H2S升高。与C组比较,D组CRP、降钙素原、内毒素、TNF-α、IL-6、IL-8升高,H2S降低(P<0.01)。B、C、D组分别有14、6、15例血标本培养出大肠杆菌。H2S与CRP、降钙素原、内毒素、TNF-α、IL-6、IL-8呈负相关。 结论 H2S降低肠缺血-再灌注损伤大鼠血炎症因子,改善内毒素血症。Abstract: Objective To investigate the influence of hydrogen sulfide on the levels of plasma C reactive protein (CRP),procalcitonin (PCT),endotoxin,TNF-α,IL-6 and IL-8 in rats with intestinal ischemic-reperfusion injury. Methods A total of 64 healthy male Wistar rats were randomly divided into sham operation group (A),ischemia-reperfusion group (B),ischemic-reperfusion and sodium hydrosulfide group (C),and ischemia-reperfusion and PPG group (D),n=16.The intestinal ischemic-reperfusion injury rat model was prepared as follow:used a midline incision for laparotomy,freed and clamped the superior mesenteric artery (SMF) for 60 min,and then reperfusion for 120 min.The tail vein injection of sodium hydrosulfide[100 μmol/kg]was performed 10 min prior to the onset of reperfusion,and continuous infusion[1 mg/(kg·h)]until reperfusion for 2 h in group C.The intraperitoneal injection of PPG (50 mg/kg) was performed in group D 10 min before the ischemia.The levels of CRP,PCT,endotoxin,H2S,TNF-α,IL-6 and 8 were tested;the synchronic blood culture was performed to evaluate the endotoxemia.H2S in the ileal homogenate was also measured. Results Compared with group A,the level of plasma endotoxin,PCT,CRP,TNF-α,IL-6 and 8 in the group B increased but H2S decreased.Compared with group B,the level of plasma endotoxin,PCT,CRP,TNF-α,IL-6 and 8 in group C decreased.Compared with C group,the level of plasma endotoxin,PCT,CRP,TNF-α,IL-6 and 8 in the group D increased.The level of plasma H2S in group D was significantly lower than that in group B and C.There were 14,6 and 15 blood samples respectively in group B,C and D infected by Escherichia colic.H2S negatively correlated with the levels of CRP,CT,endotoxin,TNF-α,IL-6 and IL-8. Conclusion H2S can decrease the level of inflammatory factor (PCT,CRP,TNF-α,IL-6 and IL-8) and attenuates endotoxinemia in rats with intestinal ischemic-reperfusion injury.
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Key words:
- Ischemic-reperfusion /
- Hydrogen sulfide /
- Endotoxin /
- Inflammatory factor
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