Abstract: Objective To investigate the clinical significance of biochemical indicators in patients with intrahepatic cholestasis of pregnancy. Methods One hundred and thirty-six cases of intrahepatic cholestasis of pregnancy from September, 2014 to September, 2016 in our hospital were selected into observation group, and 86 cases of healthy pregnant women at the same period in our hospital were selected as control group.The lipids[Triacylglycerol (TG), total cholesterol (CHOL), high-density lipoprotein (HDL), low-density lipoprotein (LDL)], liver function[total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate aminotransferase (AST), serum total bile acid (TBA)], estrogen (estradiol, estriol) of two groups were detected, and the neonatal outcome of two groups were observed.All date of two groups were statistical analyzed using statistical soft to analyze the application value of blood lipid, liver function and estrogen in intrahepatic cholestasis of pregnancy. Results The TG, CHOL, LDL levels of observation group were significantly higher than those of control group (P < 0.05), and the HDL level of observation group was significantly lower than those of control group (P <0.05).The TBIL, ALT, AST, TBA levels of observation group were significantly higher than those of control group (P <0.05).The estradiol, estriol levels of observation group were significantly higher than those of control group (P < 0.05).The premature infants, neonatal asphyxia, intrauterine distress ratio of observation group were significantly higher than those of control group (P < 0.05). Conclusion The blood lipids, liver function, estrogen of pregnancy intrahepatic cholestasis were abnormalities, timely examination and prevention would be helpful to reduce the neonatal adverse outcomes.