Abstract: Objective To investigate the changes of glycogen antigens 125 (CA125) and procalcitonin (PCT) in serum and ascites of patients with hepatitis B cirrhosis complicated with spontaneous bacterial peritonitis. Methods Sixty cases of patients with Hepatitis B cirrhosis of ascites combined with spontaneous bacterial peritonitis were selected as the observation group and 60 cases of patients with Hepatitis B cirrhosis of ascites without spontaneous bacterial peritonitis were selected as the control group.The levels of CA125 and PCT in serum and ascites were measured. Results Serum levels of CA125 and PCT in the observation group[(425.76 ±157.43) U/L, (1.326 ±0.434) ng/ml] were significantly higher than those in the control group[(314.27 ±132.95) U/L, (0.587 ±0.276) ng/ml], P < 0.05.The ascites CA125 and PCT levels in the observation group[(567.34 ±213.75) U/L, (0.983 ±0.316) ng/ml] were higher than those in the control group[(378.52 ±167.53) U/L, (0.214 ±0.105) ng/ml], P < 0.05.In the 47 patients with antibiotic therapy, the serum and ascites CA125 and PCT level after treatment[(411.53 ±152.13) U/L, (1.279 ±0.424) ng/ml; (558.76 ±202.14) U/L, (0.946 ±0.307) ng/ml] were lower than those before treatment[(331.86 ±143.25) U/L, (0.635 ±0.265) ng/ml, (385.42 ±197.47) U/L, (0.546 ±0.324) ng/ml], P < 0.05.The serum CA125 and ascites CA125 were positively correlated (r=0.882, P=0.000).There was positive correlation between serum PCT and ascites PCT (r=0.856, P=0.000).There was no significant correlation between serum CA125 level and serum PCT level (P >0.05). Conclusion The serum and ascites CA125, PCT levels in patients with Hepatitis B cirrhosis with spontaneous bacterial peritonitis increases.The levels of CA125 and PCT in serum and ascites are decreased in validated patients by antibiotic treatment.