Abstract: Objective To observe the expression of Bcl-2、Bax and caspase-3 in mouse tubular epithelial cells after cisplatin-induced cytotoxicity, before and after the treatment with resveratrol, and to explore the underlying mechanism of resveratrol against cisplatin-induced cytotoxicity. Methods Mouse proximal tubular cells (m Prox) cultured in vitro were divided into 4 groups, controls (C), cisplatin alone at 25 μM (CIS), cisplatin 25 μM plus resveratrol 100 μM (CV + RES), and resveratrol alone 100 μM (RES), 25 μM cisplain, pretreated with 100 μM resveratrol for 3 hours, cells were stimulated for 24 hours. Cell proliferation activity was detected by Trypan Blue Assay, analysis of cell apoptosis by Tunel Assay, Bcl-2, Bax and caspase-3 were detected by using Westernblot. Results Compared with the control group, cellular viability in the cisplatin group was decreased (P < 0. 05), cell apoptosis was increased (P < 0. 05), the expression of Bax, caspase-3 were increased (P < 0. 05) and the expression of Bcl-2 was decreased in the cisplatin group; resveratrol can increase cellular viability, restrain cell apoptosis (P < 0. 05), lower the activity of Bax and caspase-3 (P < 0. 05) and increase the activity of Bax (P < 0. 05). Conclusion Resveratrol can effectively increase cell viability, minimize apoptosis in mouse proximal tubular cells. The mechanism may be via down-regulating the expression of Bax, caspase-3 and increasing the expression of Bcl-2.