Abstract: Objective To explore and analyze the correlation between 3D-PDU, PLGF and sFlt-1 in placenta of preeclampsia patients. Methods From September, 2015 to September, 2016, 100 patients with preeclampsia and eclampsia were divided into mild group and severe group according to severity, 50 cases in each group, 50 cases in our hospital from other health expectant mothers, as compared to the control group. All pregnant women prenatal three-dimensional power Doppler ultrasound (3D-PDU) examination, and the use of 3D reconstruction technology and VOCAL software to measure the vascular index (VI), flow index (FI) and vascularization flow index (VFI), detection of placental growth factor after delivery (PLGF) and soluble vascular endothelial growth factor receptor-1 (sFlt-1) level, and statistical analysis of correlation between the results of 3D-PDU and PLGF in placenta and the level of sFlt-1. Results In mild group and severe group, FI and VI in placenta of patients with VFI were significantly lower than that of control group (P < 0. 05). In severe group, FI and VI in placenta of patients with VFI were significantly less than mild group (P < 0. 05), with the aggravation of disease, VI, FI, placenta VFI decreased; mild group and severe group of patients with placenta PLGF were significantly lower than the control group (P < 0. 05), severe group was significantly lower than that of PLGF in placenta of patients with mild group (P < 0. 05), with the aggravation of disease, placenta PLGF decreased; mild group and severe group of patients with placenta sFlt-1 were significantly higher than the control group (P < 0. 05), severe group was significantly higher than that of SFLT-1 in placenta of patients with mild group (P < 0. 05), with the sicker patients, placental SFlt-1 increased, with statistical significance. Pearson correlation analysis showed a significant correlation between placental VFI and placental PLGF (P < 0. 01), which was positively correlated with statistical significance. There was a significant correlation between placental VFI and placental sFlt-1 (P < 0. 01), and there was a negative correlation between them. Conclusion There is a significant correlation between the detection of 3D-PDU and the expression of PLGF and sFlt-1 in placenta of preeclampsia, which may provide some help for the clinical diagnosis of preeclampsia.