Abstract: Objective To analyze the influencing factors of hospitalization costs of patients with breast cancer, and provide the basis for allocating medical resources, reducing medical expenses and establishing reasonable medical insurance system. Methods The expenditure pattern of hospitalization costs of 1 389 cases of breast cancer in a third grade hospital in Anhui Province between January, 2011 and December, 2016 were retrospectively analyzed. The hospital information of all patients was got from hospital HIS system. ANOVA and multivariate Logistic regression analysis by SPSS 16. 0 were conducted for the related factors of hospitalization cost. The effective intervention strategies were put forward according to the results. Results Among 1 389 breast cancer patients, the average hospitalization cost was (31 425. 8 ±357. 3) yuan, the hospitalization cost was mainly composed of drug fee (42. 4%), operation fee (24. 8%), treatment fee (9. 1%) and other expenses (16. 5%). The single factor analysis showed that the number of hospitalization, days of hospitalization, mode of payment, clinical stage and treatment methods were the affecting factors of hospitalization cost (P < 0. 01). Multivariate Logistic regression analysis showed that the number of hospitalization, days of hospitalization, and clinical stage were the main factors that affecting hospitalization costs (P < 0. 05). Conclusion There are many factors that may affect hospitalization cost of patients with breast cancer, analysis of the influencing factors of hospitalization cost of breast cancer patients has great significance to control the unreasonable growth of medical cost. Health administrative department and hospital should intervene in the unreasonable growth of medical cost, so as to reduce the cost of medical treatment and economic burden of patients.