Abstract: Objective To study the clinical value of IL-1β, IL-17 A, IL-27, IFN-γ, TNF-α detection in predicting and staging of primary liver cancer patients. Methods Ninety-six primary liver cancer patients (stage Ⅰ and Ⅱ 35 cases, stage Ⅲ 29 cases, stage Ⅳ 32 cases) were selected as research subjects from August, 2012 to December, 2015. Thirty-five cases of normal healthy people were chosen to be as the control group. The serum IL-1β, IL-12p70, IL-17 A, IL-22, IL-23, IL-27, IL-2, IL-4, IL-12, IFN-γ level were detected and compared in the liver cancer group and the control group. The ROC curves were used to assess the effect of each cytokines above in predicting the primary liver cancer stages. Results The area under the ROC curve of IL-1β, IL-17 A, IL-27, TNF-α to diagnose primary liver cancer patients were 0. 857, 0. 998, 0. 982, 0. 982. The effect of forecasting the primary liver cancer patients' stage was the best, with the AUC 0. 940, cut-off value 307. 05 pg/ml, the sensitivity 87. 50%, specificity 89. 06%, positive predictive value 83. 56%, negative predictive value 75. 50%, and Youden index 0. 77. Conclusion The levels of IL-1β, IL-4, IL-17 A, IL-27, IFN-γ, TNF-α are significantly different (P < 0. 05) between the primary liver cancer patients and healthy people, and IL-27 has a good future in diagnosing the stage of primary liver cancer patients.