Abstract: Objective To investigate the characteristics and drug sensitivity of carbapenem-resistance Enterobacteriaceae (CRE) infection in our hospital, and study the genotypic analysis of carbapenemase genes. Methods A total of 140 strains of CRE were collected and the epidemiological date were analyzed. CRE were identified by using VITEK 2-Compact bacterial identification system, the antibiotics-sensitivity were tested by kirby-bauer disk susceptibility test, carbapenemase production was detected by the Modified Hodge Test (MHT), and the carbapenemase genes (KPC, IMP, VIM, NDM, OXA-48) were detected by PCR technology. Results Klebsiella pneumoniae was dominated in all CRE, accounting for 70. 71%, followed by Enterobacter aerogenes (6. 44%) and Enterobacter cloacae (5. 71%). CRE samples were mainly isolated from sputum, accounting for 69. 29%, followed by urine (12. 86%), secretion (4. 29%) and blood (4. 29%). The most of the patients were from ICU, accounting for 44. 29% and followed by neurosurgery (18. 57%) and hematology (5. 00%). The drug sensitivity test showed CRE were high resistant to most of β-lactam antibiotics, the resistant rate was higher than 80%, the resistant rate to minocyline and amikacin were 14. 78% and 35. 43%, respectively.Modified Hodge test showed 127 strains were positive. PCR and gene sequencing showed 99 strains CRE carrying KPC-2, 14 strains carrying IMP-4, 2 strains carrying IMP-1 and 13 strains carrying NDM-1. Conclusion CRE is highly resistant to most antibiotics in our hospital, and the main resistant gene is KPC, followed by IMP and NDM. According to susceptibility test, the hospital should enhance the drug-resistance surveillance and use antibiotics reasonable to control the multi-drug resistance and the prevalence of resistant strains.