Abstract: Objective To investigate effect of animal experiment by ultrasound combined targeted microbubbles[homemade, consist of contrast medium (fluorocarbon), vascular endothelial growth factor₁₆₅ complementary deoxyribose nucleic acid (VEGF₁₆₅ c DNA), anti-intercellular adhesion molecule (ICAM-1) monoclonal antibody]mediated therapy for cerebral infarction, and to observe effect by VEGF₁₆₅ c DNA gene transfection for improving damaged endangium. Methods A total of 60 New Zealand white rabbits were taken into high fat dietingcerebral infarction model, and randomly divided into three groups. They respectively received ultrasound combined targeted microbubbles (group A), actilyse (group B), and normal saline (control gorup) for treatment. Revascularization, neutrophil infiltration in infarction necrotic zone, and infarction area quality proportion were observed in each group. Results After treatment, groups A and B had better revascularization condition than control group (P < 0. 05), while there was no statistically significant difference of revascularization condition between groups A and B (P > 0. 05). Group A had obviously less average neutrophil count in infarction necrotic zone than group B after treatment (P < 0. 05), but the difference of average neutrophil count in infarction necrotic zone had no statistical significance between group B and control group (P > 0. 05). Infarction area quality proportion after treatment was much lower in group A than in group B (P < 0. 05), and that in group B was also lower than that in control group (P <0. 05). Conclusion Ultrasound combined targeted microbubbles mediated provides precise effect in adjuvant therapy for VEGF₁₆₅ c DNA gene transfection in animal experiment of cerebral infarction. This method can remarkably improve revascularization, decrease inflammatory reaction in infarction area, neutrophil infiltration and infarction area quality proportion.It is beneficial for cerebral infarction treatment.