Abstract: Objective To investigate the role of rapid detection of enterovirus Ig M on improving the nursing management of children with hand-foot-mouth disease. Methods Total 126 patients who met the diagnostic criteria for hand-footmouth disease in May, 2015 were enrolled into this study with randomized controlled trials (RCTs). All the patients were assigned into non-pathogenic rapid diagnosis group (Group Ⅰ, 62 cases) and pathogenic rapid diagnosis group (GroupⅡ, 64 cases). In group I, the stool and throat swabs specimen were prepared for PCR test; the patients were uniformly assigned into wards and received the routine nursing care. In group Ⅱ, the tests for Ig M antibody (colloidal gold assay) were additionally performed, and the patients were assigned into isolation ward according to the different pathogens, and received individualized care. The differences of pathogen, results of PCR and antibody tests, hospitalization time and rate of re-infection within two weeks were compared. Results There was no statistical difference of pathogen between the two groups (P=0. 927), and the corresponding accuracy rate of between PCR and antibody tests was 98. 4%. The hospitalization time of Group Ⅱ was shorter than that of Group Ⅰ (P < 0. 001). There were 8 re-infection cases in Group Ⅰ, while 0 cases in Ⅰ group, the difference was significant differences (P < 0. 001). Conclusion The colloidal gold assay method is effective in the detection of hand-foot-mouth disease virus Ig M. According to the results of rapid pathogenic tests, the medical personnel can assign patients with different pathogens into isolation wards, and provide individualized care and treatment, thus will improve the pertinence of treatment and care and the quality of nursing management, shorten the hospitalization time, also reduce secondary infection in the ward.