Abstract: Objective To investigate the clinical value of echocardiogram and myocardial enzymes detection in patients with acute pulmonary embolism(APE). Methods A total of 60 acute pulmonary embolism patients were selected as research group(n=60) and 60 physical examination individuals as control group(n=60).The echocardiogram(EDV,ESV,EF,PER and PFR) and levels of myocardial enzymes(CK,CK-MB,LDH and AST) were tested.The pulmonary embolism-associated deterioration and death were compared between the patients with right ventricular dysfunction and the subjects with normal right heart function. Results Compared with the control group,EDV and ESV of the research group were significantly higher(P<0.05),EF,PER and PFR were significantly lower than those in control group,but SV has no significant difference between the two groups(P>0.05);The levels of CK,CK-MB,LDH and AST of the research group were significantly higher than those in the control group(P<0.05);In the 60 patients with acute pulmonary embolism,29 patients were with ventricular dysfunction,the incidence of pulmonary embolism-associated deterioration and mortality in these patients were significantly higher than the individuals with normal right heart function(P>0.05). Conclusion Echocardiography can help to evaluate right ventricular function in patients with acute pulmonary embolism,while myocardial enzymes detection will contribute to assess myocardial injury.Echocardiography and myocardial enzyme detection may be helpful to the diagnosis and prognosis evaluation of patients.