Abstract: Objective To study the distributions and drug resistance of clinical isolated K.pneumoniae from a hospital of Traditional Chinese Medicine(TCM),and provide scientific evidence for anti-infective therapy and nosocomial infection control. Methods The K.pneumoniae strains were isolated from clinical specimens between January,2013 and December,2015 in a hospital of TCM.The drug sensitivity test was performed by using K-B method and MIC method,and all of data was analyzed by using WHONET 5.6 software. Results A total of 1 939 strains of K.pneumoniae were isolated from clinical specimens.The top three clinical specimens were sputum(72.98%),urine(12.74%) and blood(4.74%).Department of Respiratory(15.58%),Department of Gerontology(10.83%),Nephrology Department(7.43%) and ICU(6.45%)accounted for the highest proportion.The drug resistance rates of K.pneumoniae to piperacillin were highest(36.62%),and the rates to cefuroxime,cefotaxime,ceftriaxon,SMZ-TMP and ampicillin/sulbactam were all more than 30%.The resistance to carbapenem and β-lactamase inhibitor compound were less than 15%.The resistant rates to imipenem were 10.30%,9.70% and 11.81% respectively in recent 3 years,and to Meropenem were10.41%,10.00% and 10.94%,respectively. Conclusion Klebsiella pneumoniae isolated from this hospital of TCM shows a elevated resistance rates to second and third generation cephalosporins,and the resistance rates to carbapenems have a trend of escalation.To reduce the production of multi-drug resistant bacteria,antibiotics,especially carbapenems,should be used cautiously and rationally.