Abstract: Objective To observe the effect of psychological intervention on serotonin and anxiety scores in patients with ulcerative colitis,thus to guide the treatment. Methods All 72 cases were selected from January,2016 to December,2016 diagnosed in our hospital as digestive ulcerative colitis,and were randomly divided into observation group and control group.Based on different symptoms and severity of the disease,we made individualized medication plan.The observation group had psychological intervention in addition to clinical medication.With 36 cases in each group,after 14 days of treatment we used SAS and SDS scales to compare anxiety and depression before and after treatment,and serotonin expression in mucosal mucosa before treatment and 2 months after treatment. Results There was no significant difference in SAS scores between the two groups before treatment(P>0.05).SAS scores were lower in both groups after treatment(P<0.05).The improvement rate of SAS in the observation group was better than that in the control group(P<0.05).There was no significant difference in the scores of SDS scores between the two groups before treatment(P>0.05).The scores of SDS in the observation group and the control group were lowered after treatment(P<0.05).And the level of improvement in the observation group outperformed that in the control group(P<0.05).There was no significant difference in the expression of serotonin between the two groups before treatment(P>0.05).The levels of serotonin in both groups were lowered after treatment(P<0.05).The improvement level of the observation group was better than that of the control group(P<0.05). Conclusion Psychological intervention can alleviate the anxiety of patients with ulcerative colitis and improve the symptoms of gastrointestinal dysfunction by inhibiting the expression level of serotonin in the intestine,which is worthy of clinical application.