中华全科医学

Current situation and future development of general stomatology

CHEN Xing, LIU Qi, CHEN Feng, DING Cheng

2022, 20(5): 721-724. doi: 10.16766/j.cnki.issn.1674-4152.002439

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General stomatology is a brand-new development form in the field of stomatology, which originated from general practice and is the embodiment of general practice in the field of stomatology. In China, the concept of general dentistry was clearly put forward in 2009, the general dentistry range, the requirements of practitioners of the corresponding specification, promote the department of stomatology in general hospital, private dental clinics, and even the national general dentistry concept within the scope of application. With the progress of society and the development of stomatology, patients are becoming increasingly demanding for general dentistry. Under the patient-oriented modern clinical diagnosis and treatment mode, oral multidisciplinary team (OMDT) has emerged. OMDT can comprehensively evaluate patient's disease situation, specialization, standardization and personalized provides the best comprehensive treatment for patients. OMDT aims to restore the mouth-jaw system function and overall health. It is an important development model of general dentistry in China in the future. In order to further explore general stomatology, this paper makes a corresponding summary and reasonable discussion on the development and current situation of general stomatology, and puts forward the problems existing in the development of general stomatology, so as to propose scientific and reasonable suggestions to promote the further development of general stomatology.

The Doctor-Patient Relationship in United Kingdom General Practice(to be continued)

Rodger Charlton, MAO Jing, LIU Man-ling

2022, 20(5): 725-726.

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Clinical value of serum human epididymal protein 4 in the diagnosis of chronic kidney disease

LI Xiu-yi, QI Xiang-ming, XIA Yuan-yuan, LUO Rui, HAN Shu-wei, YU Xiao-qin

2022, 20(5): 789-792. doi: 10.16766/j.cnki.issn.1674-4152.002455

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Objective To investigate the clinical diagnostic value of serum human epididymal protein 4 (HE4) in chronic kidney disease (CKD) and different stages. Methods A total of 117 patients with CKD admitted to the Department of Nephrology of Hefei First People's Hospital from February 2019 to September 2020 were selected as the observation group, and 78 healthy patients with the same age during the same period were selected as the control group. The expression levels of serum HE4, urea nitrogen (BUN), creatinine (SCr), cystatin C (CysC) and retinol-binding protein (RBP) in the subjects of the two groups were detected to observe the correlation and clinical diagnostic value of CKD. Results The expression levels of serum HE4, BUN, SCr, CysC and RBP in the CKD group were significantly higher than those in the control group (all P < 0.01). As the CKD stage of the patients increased, no significant difference in the early grouping of BUN, SCr and RBP was found compared with the control group in each stage of CKD and the control group (all P>0.05). The other staging groups were compared with the control group, and the differences were statistically significant (all P < 0.01). The comparison amongst the CKD 1 - 5 staging groups revealed that the HE4, CysC and RBP groups had statistically significant differences (all P < 0.05), and BUN and SCr were significantly different (all P < 0.05). Compared between groups, there was no significant difference between CKD 1 and CKD 2 (all P>0.05), and significant differences were noted between the CKD 3 and CKD 5 groups and compared with CKD 1 to CKD 2 (all P < 0.01). The expression level of serum HE4 was negatively correlated with eGFR but positively correlated with BUN, SCr, CysC and RBP. ROC analysis showed that the area under the curve of HE4 (AUC=0.978) was significantly better than that of Bun (AUC=0.956), SCr (AUC=0.973), CysC (AUC=0.992) and RBP (AUC=0.910). Conclusion The serum HE4 level is related to the occurrence and development of CKD, and it has certain clinical significance for the staging of CKD. Serum HE4 is expected to function as a new serum biological diagnostic marker for the progression and prognosis of CKD.

Application value of bicycle exercise therapy and gabapentin in the treatment of haemodialysis patients with restless legs syndrome

XIA Min, CHEN Fu-xing

2022, 20(5): 793-795. doi: 10.16766/j.cnki.issn.1674-4152.002456

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Objective To analyse the effects of cycling exercise therapy and gabapentin in the treatment of haemodialysis patients with restless legs syndrome (RLS), and determine their effects on sleep quality and quality of life. Methods Sixty haemodialysis patients with RLS admitted in People's Hospital of Beilun District from December 2018 to June 2020 were randomly divided into the observation group and control group. The control group was treated with gabapentin, and the observation group was treated with bicycle exercise therapy and gabapentin. The clinical efficacy, sleep quality and quality of life were investigated before and after 12 weeks of treatment. Results After treatment, the IRLS scale score, AIS scale score and PSQI scale score of the observation group were significantly lower than those of the control group [(7.03±1.83) points vs. (13.64±2.11) points, (4.12±1.11) points vs. (5.99±0.94) points, (7.03±0.75) points vs. (9.13±0.88) points, respectively], and the differences were statistically significant (all P < 0.05). After treatment, SF-36 psychological dimension scores and SF-36 physiological dimension scores of patients in the observation group were higher than those in the control group [(78.12±6.22) points vs. (70.04±5.33) points, (79.98±7.48) points vs. (69.82±6.32) points, respectively], and the differences were statistically significant (all P < 0.05). Conclusion The combined application of bicycle exercise therapy and gabapentin can significantly improve the efficacy of haemodialysis patients with RLS, as well as improve the quality of sleep and quality of life.

Effect of preoperative total cholesterol and high-density lipoprotein-cholesterol levels on the prognosis of breast cancer

LI Cheng-wei, QIAO Meng-xiang, LIU Tie-cheng, JIN Gong-sheng

2022, 20(5): 796-800. doi: 10.16766/j.cnki.issn.1674-4152.002457

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Objective To determine serum total cholesterol (TC) and high-density lipoprotein-cholesterol (HDL-C) of patients with breast cancer, explore the effect of preoperative blood lipid levels on the prognosis of patients with breast cancer. Methods Fasting plasma lipid in the morning and clinical pathological data of 341 patients with breast cancer confirmed by pathology from January 2012 to December 2014 in the First Affiliated Hospital of Bengbu Medical College were collected retrospectively. Kaplan-Meier analysis and Cox proportional hazard regression model were used to analyse overall survival (OS) and disease-free survival (DFS). Results The average OS of patients with TC < 6.2 mmol/L was 96.2 months, whereas that of patients with TC≥6.2 mmol/L was 95.4 months (P=0.556). The average DFS of patients with TC < 6.2 mmol/L was 95.9 months, and that of patients with TC≥6.2 mmol/L was 94.0 months (P=0.122). In different levels of TC groups, the OS rate and DFS rate were not statistically significant. The average OS of patients with HDL-C < 1.04 mmol/L was 94.7 months, whereas that of patients with HDL-C≥1.04 mmol/L was 97.6 months (P=0.019). The average DFS of patients with HDL-C < 1.04 mmol/L was 93.2 months, whereas the average DFS of patients with HDL-C level≥1.04 mmol/L was 94.7 months (P=0.003). In different levels of HDL-C groups, the OS time and DFS time were statistically significant. COX multivariate analysis showed that high HDL-C was beneficial to DFS (HR=3.916, 95% CI: 1.355-11.313, P=0.012). Conclusion The preoperative HDL-C level may be an independent factor predicting DFS in patients with breast cancer.

Expression of miRNA-107 in peripheral blood of children with attention deficit hyperactivity disorder

JIANG Mao-ying, WANG Zun-hui, CHEN Dong, HUANG Hong-yu, ZHU Ping

2022, 20(5): 801-804. doi: 10.16766/j.cnki.issn.1674-4152.002458

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Objective To measure the expression levels of miRNA-107 and miR-34c-3p in children with attention-deficit hyperactivity disorder (ADHD). Methods From January 2018 to September 2019, 37 children were admitted to the Developmental Behaviour Clinic of Children's Health Care in Hangzhou Children's Hospital and were set as the ADHD group. Seventy-four healthy children in our hospital were set as the healthy control group. The relative expression levels of miRNA-107 and miR-34c-3p in peripheral blood were detected by quantitative reverse transcription-polymerase chain reaction. The sensitivity and specificity of miRNA-107 in diagnosing ADHD were analysed according to the receiver operating characteristic (ROC) curve. The correlation between miRNA-107 expression and ADHD core symptoms was analysed. Results The relative expression of miRNA-107 in peripheral circulation was significantly lower in the ADHD group than in the control group (4.06±1.57 vs. 4.62±1.30, t=1.994, P=0.049), whereas miR-34c-3p showed no significant difference. The AUC of miRNA-107 was 0.609, the sensitivity was 97.3% and the specificity was 67.6%. Correlation analysis showed that the expression level of miRNA-107 in ADHD girls was negatively correlated with the degree of attention deficit (r=-0.861, P < 0.05). Conclusion MiRNA-107 may be a potential biomarker of ADHD and has a certain diagnostic significance.

Expression of miRNA-155 in children with recurrent wheezing and its relationship with Th1/Th2 cell balance

SHEN Ren, YANG Shan-pu, WU Yue-chao, ZHOU Yan, ZHANG Lin-tao

2022, 20(5): 805-807. doi: 10.16766/j.cnki.issn.1674-4152.002459

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Objective To analyse the expression of miRNA-155 in children with recurrent wheezing and to explore the possible relationship between miRNA-155 and wheezing and the balance of Th1/Th2 cells. Methods Forty cases of children with recurrent wheezing in Yuhuan People's Hospital from January 2019 to October 2020 were selected as observation group, and 40 cases of healthy children in our hospital at the same time were selected as control group. The levels of miRNA-155, IFN-γ and IL-4 in the peripheral blood of the two groups were detected. Results The expression of miRNA-155 in the observation group was 5.24(3.42, 7.45), and that in the control group was 6.52(4.51, 12.88). The expression of miRNA-155 in the observation group was significantly lower than that in the control group (P < 0.05). The IFN-γ/IL-4 value of the observation group was 0.73±0.09, and that of the control group was 0.82±0.10, and the difference was statistically significant (P < 0.05). The expression of miRNA-155 was negatively correlated with the number of wheezing in the observation group (r=-0.557, P < 0.05). No correlation was observed between the expression of miRNA-155 and IFN-γ/IL-4 value in the observation group (r=0.223, P>0.05). Conclusion The expression of miRNA-155 may be negatively correlated with the occurrence of wheezing in children, and the decreased expression of miRNA-155 may increase the risk of wheezing in children. Decreased IFN-γ-to-IL-4 ratio may increase the risk of wheezing in children, but there is no significant correlation between the expression of miRNA-155 and the balance of Th1/Th2 cells.

Diagnosis cognition and influencing factors of Parkinson disease among community general practitioners in Shanghai

ZHAO Ya-jun, TIAN Jiu-peng, WANG De-qiang, ZHANG Fang-yan, YANG Sen, GE Xu-hua

2022, 20(5): 808-811. doi: 10.16766/j.cnki.issn.1674-4152.002460

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Objective To investigate the diagnosis cognition of Shanghai community general practitioners on Parkinson disease and analyze its influencing factors, in order to provide a reference for improving the awareness and ability of general practitioners in early recognition of Parkinson disease. Methods The questionnaire survey method was used from September 25 to October 28, 2020. A total of 553 community general practitioners in Shanghai were selected as the research objects, and the self-designed questionnaire was used to conduct an electronic questionnaire survey. The χ² test and Mann-Whitney U test were used to compare the characteristics of general practitioners at different levels of cognition, and the multi-factor logistics regression method was used to explore the influencing factors. Results Only 38.1% (210/551) of general practitioners believed that Parkinson disease could be diagnosed in the community, and 61.9% (341/551) of them believed that it was not possible. The top three reasons were as follows: 60.4% (206/341) of the motor and non-motor symptoms of Parkinson disease were not mastered; 49.6% (169/341) of Parkinson disease needed special equipment to diagnose; 46.0% (157/341) of them could not understand the Parkinson disease guidelines due to lack of professional background. The influencing factors were professional title (OR=1.555, 95% CI: 1.160-2.086, P < 0.05), whether they knew the relevant diagnosis and treatment guidelines (OR=3.167, 95% CI: 2.186-4.589, P < 0.05), and whether there was neurology specialist clinic (OR=1.702, 95% CI: 1.127-2.571, P < 0.05). Conclusion General practitioners ' cognition of the diagnosis of Parkinson disease is obviously insufficient. The awareness and ability of early recognition of Parkinson disease needs to be improved. The influencing factors include professional title, whether they know the relevant diagnosis and treatment guidelines, and whether there is neurology specialist clinic. The community health service centers can establish neurology specialist clinics, and give priority to the intervention in the form of outpatient teaching combined with the needs of general practitioners, in the hope of improving the early diagnosis rate of Parkinson disease among community residents.

Analysis and evaluation of quality control of community general practice in Pudong New District, Shanghai

SONG Hui-jiang, QIN Wei, HUANG Xi-ya, YANG Li-ping

2022, 20(5): 812-815. doi: 10.16766/j.cnki.issn.1674-4152.002461

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Objective To analyse the quality control evaluation results of community general practice in Pudong New Area, Shanghai, in order to determine the direction for the optimised development of community general practice in Pudong New Area. Methods From May 6 to 31, 2019, a quality control team was organised by the person in charge of Shanghai East District to conduct general practice quality control evaluations on 47 community health service centres in Pudong New District, through field investigations. The assessment content included the basic conditions of the institution, staffing and job responsibilities of general practitioners, general medical quality management, disease diagnosis and treatment capabilities of general practitioners, chronic disease management and treatment capabilities of general practitioners, education and training of general practitioners, and additional items of scientific medical research and teaching status. Results The average standard score of 47 community health service centres in Pudong New Area was (87.9±4.7) points, and the total score was (90.2±6.0) points. The number of institutions with total score of C was the largest [29 (61.7%)]. Compared with the city's average level, the education and training of general practitioners in Pudong New Area ranked first in the city, whereas the rest were without obvious advantages. The disease diagnosis and treatment capabilities of general practitioners and the additional points of scientific research and teaching were lower than the city's average; the disease diagnosis and treatment capability scores of urban and suburban general practitioners were the lowest in the city. Correlation analysis results showed that scientific research and teaching was positively correlated with the staffing and job responsibilities and disease diagnosis and treatment capabilities of general practitioners (all P < 0.05). The results of the difference analysis showed that there was a statistically significant difference in the scientific medical research and teaching status scores between the urban and suburb (P < 0.05). Conclusion In the quality control of community general practice in Shanghai, Pudong New Area fails to show the 'Pudong' brand effect, and some core competencies (diagnosis and treatment capabilities of general practitioners, scientific research and teaching) even shows poor performance. It is necessary to implement hierarchical diagnosis and treatment in the region, complete the performance evaluation of general practice and increase scientific research investment and support. At the same time, attention should be paid to scientific research and teaching and clinical practice, and the balance of the urban and suburb development, so as to comprehensively improve the current poor situation.

Evaluation of the effect of the "Internet plus" health management model core with comics on the management of young and middle-aged people at high risk of cardiovascular disease

LI Xia, CHEN Yu-jiao, CHEN Li-li, CHENG Liu, LU Chun-yu, XU Xiao-ying

2022, 20(5): 816-820. doi: 10.16766/j.cnki.issn.1674-4152.002462

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Objective To explore the application effect of the new 'Internet plus' health management model with comics as the core (new management for short) in the management of young and middle-aged people at high risk of cardiovascular disease (high-risk population for short). Methods A total of 118 cases were selected from office buildings around a street in Shanghai from February through April 2019 as the research objects. They were divided into a new management group (n=59) and a conventional management group (n=59) by simple random grouping. All personnel completed the health knowledge test after joining the group and carried out health management for six months. After 6 months, the two groups filled out the risk assessment questionnaire and health knowledge test again. The physical and chemical indexes, knowledge and behaviour indexes, loss to follow-up rate and satisfaction rate of the two groups were compared and analysed. Results After the intervention, the reduction of BMI [1.3 (0.9, 1.6) vs. 0.9 (0.3, 1.3)], systolic blood pressure [20.0 (14.5, 23.0) mm Hg vs. 16.0 (12.0, 19.0) mm Hg, 1 mm Hg=0.133 kPa], diastolic blood pressure [10.0 (14.5, 23.0) mm Hg vs. 8.0 (5.0, 9.0) mm Hg], LDL-C [1.1 (0.7, 1.3) mmol/L vs. 0.7 (0.2, 1.0) mmol/L], the frequency of blood pressure monitoring and the health knowledge awareness level in the new management group were higher than those in the conventional management group (all P < 0.05). Moreover, significant differences were observed in the health knowledge awareness level before and after the intervention between the two groups (P < 0.05). After the intervention, the smoking volume in the new management group was lower than that before the intervention (P < 0.001). The loss to follow-up rate in the new management group was lower than that in the conventional management group (P < 0.05). The proportion of service satisfaction was higher than that in the conventional management group (P < 0.05). Conclusion Applying the new management model to the health management of high-risk population can increase their understanding of diseases and their own status. It can also improve the control level of blood pressure, blood lipids, BMI and smoking, enhance compliance, and improve satisfaction.

Quality of life in patients with recurrent schizophrenia and analysis of influencing factors

YU Lin-hua, WANG Tie-hu

2022, 20(5): 821-823. doi: 10.16766/j.cnki.issn.1674-4152.002463

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Objective To analyse the quality of life of patients with recurrent schizophrenia and its influencing factors. Methods A total of 82 patients with recurrent schizophrenia in Hangzhou Seventh People's Hospital from February 2017 to February 2020 were selected as the research subjects. The quality of life status of the patients and the related factors affecting the quality of life of the patients were investigated. Results Compared with the Chinese norm, the scores of the patients in physiological, psychological, social relationship and environment were significantly lower (all P < 0.01). In the fields of physiology and psychology, the scores of patients with junior high school education or below were significantly lower than those with senior high school education or above (all P < 0.05). In terms of social relationship, the scores of female and non-occupational patients were significantly lower than those of male and occupational patients (all P < 0.05). On the basis of the environment, the scores of females, non-occupational, junior high school or below education level were significantly lower than those of males, occupational, senior high school or above education level (all P < 0.05). The social support of patients was positively correlated with physiological field, psychological field and environmental field (all P < 0.05), and the family care index was significantly positively correlated with each research field (P < 0.01). Drug compliance was positively correlated with the physiological and psychological fields (P < 0.01). Multiple stepwise regression analysis showed that gender, education level, social support, occupation, family care index and medication compliance were the main factors affecting patients' quality of life (all P < 0.05). Conclusion Quality of life in patients with recurrent schizophrenia is below the norm. Gender, family care index, social support, education level, medication compliance and occupational status were the main factors affecting patients' quality of life.

Application of human cartilage glycoprotein 39 and alpha-fetoprotein combined ultrasound in prenatal diagnosis of implanted placenta previa

YAO Kou-yun, DU Dan-li, LI Xiang

2022, 20(5): 824-827. doi: 10.16766/j.cnki.issn.1674-4152.002464

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Objective To evaluate the value of human cartilage glycoprotein-39 (YKL-40) and alpha fetoprotein (AFP) combined with ultrasound in placenta previa implantation. Methods A total of 90 pregnant women with placenta previa who delivered in the First Affiliated Hospital of Bengbu Medical College from November 2020 to May 2021 were enrolled as the research group. According to the intraoperative and/or postoperative pathological results, they were divided into the implantation group (4 cases of penetrating type, 17 cases of implantation type and 29 cases of adhesion type), 50 cases of non-implantation group and 40 cases of non-implantation group. In addition, 40 normal pregnant women in the same period were collected as the control group, and all received YKL-40, AFP and ultrasound examination. Results The levels of YKL-40 and AFP in the non-implantable group and control group were lower than those in the implantable group (all P < 0.05). The levels of YKL-40 in the implantable group were lower than those in the implantable group, and those in implantable group were lower than those in the penetrating group (all P < 0.05). AFP in the implantable group and implantable group were lower than those in the penetrating group (all P < 0.05). ROC curve analysis showed that the optimal cut-off values of serum YKL-40 and AFP were 320.284 pg/mL and 277.895 μg/mL, respectively, and the areas under the curve were 0.780 and 0.812, respectively. The AUC (0.895), Yuden index (0.675), sensitivity (90.0%) and negative predictive value (85.3%) of combined diagnosis were higher than those of single tests. Conclusion Serum YKL-40 and AFP levels combined with ultrasound can improve the diagnostic efficiency of placenta previa.

The expression level of serum miR-96 and miR-424-5p in patients with colorectal cancer and its clinical significance

WANG Mei, ZHANG Lin, CUI Fa-cai, WU Xiao-yu

2022, 20(5): 828-831. doi: 10.16766/j.cnki.issn.1674-4152.002465

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Objective To investigate the relationship between the expression of serum miR-96 and miR-424-5p and the clinicopathological characteristics and prognosis of patients with colorectal cancer (CRC), as well as explore the diagnostic value of serum miR-96 and miR-424-5p detection in CRC. Methods The serum samples of 120 patients with CRC, 40 patients with colorectal adenomas (CRAs) and 40 healthy controls from January to December 2015 in Henan Provincial People's Hospital were collected. The expression levels of miR-96 and miR-424-5p were detected by real-time quantitative PCR (RT-PCR). The relationship between the expression of miR-96 and miR-424-5p and the clinicopathological characteristics or the prognosis of patients with CRC was analysed. The receiver operating characteristic (ROC) curves were constructed to evaluate the diagnostic efficacy of serum miR-96 and miR-424-5p. Results The expression levels of serum miR-96 and miR-424-5p in patients with CRC were significantly higher than those in patients with CRA or healthy controls, with statistically significant differences (all P < 0.05). The expression level of serum miR-96 in patients with TNM stage Ⅲ/Ⅳ, low differentiation and distant metastasis was higher than those in patients with TNM Ⅰ/Ⅱ, moderate or high differentiation and without distant metastasis (all P < 0.01). The expression level of serum miR-424-5p in patients with TNM stage Ⅲ/Ⅳ was higher than that in patients with TNM Ⅰ/Ⅱ (all P < 0.01). The 5-year survival rate and mean survival time of patients with high expression of serum miR-96 and miR-424-5p were lower than those with low expression of serum miR-96 and miR-424-5p (all P < 0.01). The area under the curve (AUC) and specificity of miR-96 versus miR-424-5p in CRC were 0.727 and 71.2% versus 0.720 and 86.2%, respectively. The combined detection of AUC or specificity was 0.780 and 93.7%, which was greater than any single detection (all P < 0.05). Conclusion The increased expression levels of serum miR-96 and miR-424-5p were correlated with the occurrence, development and poor prognosis of CRC. The combined detection of serum miR-96 and miR-424-5p has significant reference value for the diagnosis of CRC.

Research progress of programmed cell death-1 and its ligand in autoimmune diseases

XU Li, NIE Liu-yan, WANG Hu-yan, DU Yan, XUE Jing

2022, 20(5): 832-835. doi: 10.16766/j.cnki.issn.1674-4152.002466

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Autoimmune disease is a disease wherein the body reacts to its own antigen and then attacks and damages tissues. This phenomenon is mainly caused by the activation of T cells. However, other immune cells such as B cells and dendritic cells also participate in the process of the disease. As a negative costimulatory molecule, programmed cell death-1(PD-1) is a 50-55 kDa transmembrane protein encoded by programmed cell death-1, gene on chromosome 2. It is a new member of the immunoglobulin gene superfamily isolated from apoptotic cells by subtractive method. It belongs to the immunoglobulin superfamily with inducible costimulator, CD28 and cytotoxic T-lymphocyte-associated protein 4. PD-1 has two ligands: programmed cell death-ligand 1(PD-L1) and PD-L2. By combining with their ligands, PD-1 can inhibit the activation of T cells and the production of cytokines, which plays an extremely important role in maintaining peripheral immune tolerance. PD-1 has two expression forms: membrane binding protein and soluble protein. Amongst them, membrane PD-1 plays a negative immunoregulation role by directly binding with ligands, whereas soluble PD-1 has the function of binding with PD-Ls, blocking the binding between membrane PD-1 and PD-Ls and interfering with PD-1/PD-Ls-mediated T cell proliferation inhibition. In recent years, PD-1 antibody has shown excellent efficacy in the targeted treatment of malignant tumours. Rheumatic complications such as arthritis, myalgia, secondary Sjogren's syndrome and vasculitis have also been observed in patients with autoimmune disease. With the establishment of PD-1 gene deficient mouse model, more and more research institutions are paying attention to the role of the PD-1 signalling pathway in autoimmune diseases. This paper summarises the research progress of PD-1 and its ligand in autoimmune diseases.

Application of constitution identification in community hypertension management

ZHU Qiong, CHEN Chun-feng

2022, 20(5): 836-838. doi: 10.16766/j.cnki.issn.1674-4152.002467

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Hypertension is a common chronic disease in the community, which has high incidence rate and risk. The focus of effective prevention and management of hypertension is on community chronic disease management. The traditional constitution theory of traditional Chinese medicine (TCM) clarifies the internal relationship between various constitutions and the occurrence and development of diseases. Many investigations have found that the incidence, disease changes and disease outcomes of patients with hypertension are closely related to their own constitution. Therefore, this paper first expounds the relationship between physical factors and the occurrence and development of hypertension and summarises the results of most current investigations and studies. Phlegm dampness and yin deficiency are the most common biased constitution of patients with hypertension. Secondly, the study analyses the main problems and deficiencies in the current situation of community hypertension management mode: lack of effective, scientific and reasonable prevention and treatment and lack of "individualised" management. This paper focuses on the application status and results of TCM constitution identification in community hypertension management in China, so as to clarify the significance of constitution identification in community hypertension management. Finally, the main problems of applying constitution identification to community hypertension management and perfecting this model in the future are explained, such as lack of policy support, information standardisation means and lack of TCM talents. Moreover, the problems that need to be solved and discussed are put forward. Conclusion: the constitution of TCM has an important impact on the incidence and development of hypertension. According to the theory of constitution of TCM, the use of constitution identification, appropriate technology of TCM and timely correction of biased constitution will play a certain role in delaying the occurrence and development of hypertension and preventing the occurrence of target organ damage. The constitution management of TCM provides a reference for the prevention and control of hypertension.

Progress in the treatment of chronic nonspecific low back pain with exercise therapy

ZOU Yang, SHENG Li, WU Zong-hui

2022, 20(5): 839-843. doi: 10.16766/j.cnki.issn.1674-4152.002468

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Abstract:
Low back pain is a common health and social problem that occurs at all ages, with the highest incidence in the middle and old ages. According to the duration, low back pain can be divided into acute, subacute, and chronic low back pain. Most of them are chronic nonspecific clinically. Chronic nonspecific low back pain lasts for more than 12 weeks without a clear pathoanatomical cause. No specific evidence-based methods are recommended for clinicians to cure chronic nonspecific low back pain. Given of its unclear pathogenic factors, long duration, and prognostic recurrence, the quality of life of patients is seriously affected, resulting in a huge economic burden. The treatment of chronic nonspecific low back pain is numerous and varied, and no high-quality evidence study has shown which treatment is the best. Amongst them, exercise therapy has the characteristics of low harm, low cost, and easy implementation and has thus become the mainstream clinical treatment for improving chronic nonspecific low back pain. Exercise therapy is recommended for the treatment of chronic low back pain in the Chinese expert consensus on acute/chronic nonspecific low back pain and the American College of Physicians' clinical practice guidelines. However, the evidence is insufficient to determine which specific exercise method is more effective in treating chronic nonspecific low back pain at present. Exercise therapy primarily includes core-stability training, resistance training, aerobic exercise, stretching, Pilates, yoga, Tai Chi, and McKenzie therapy. With further research, the study on the exercise treatment of chronic nonspecific low back pain has increased. Using related literature, this paper systematically reviews the progress of domestic and foreign scholars on the treatment of chronic nonspecific low back pain with exercise therapy in recent years.

Evaluation of the effect of LEI's Yinzhan ginger moxibustion combined with Buhuo Shengtu therapy on ulcerative colitis in the remission stage

XU Bao-cai, CHEN Wei

2022, 20(5): 844-847. doi: 10.16766/j.cnki.issn.1674-4152.002469

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Objective To evaluate the effect and possible mechanism of LEI's Yinzhan ginger-padded moxibustion combined with Buhuo Shengtu therapy in the treatment of ulcerative colitis (UC) in the remission stage. Methods From January 2017 to June 2020, 73 UC patients in the Department of Gastroenterology, Department of Anus and Intestine of Quzhou Hospital of Traditional Chinese Medicine were randomly divided into two groups according to random number table method. The observation group(n=36) was treated with LEI's Yinzhan ginger-padded moxibustion combined with Buhuo Shengtu therapy, whereas the control group(n=37) was treated with mesalazine. The course of treatment was 4 weeks. The intervention effect and mechanism of the two groups were evaluated. Results After treatment, the efficacy of the observation group was better than that of the control group (Z=2.352, P=0.019). The symptom score of the observation group was lower than that of the control group [(26.04±5.37) scores vs. (32.23±6.45) scores, P < 0.05]. The Mayo score in the observation group was lower than that in the control group [(5.32±1.07) scores vs. (6.75±1.56) scores, P < 0.05]. The levels of serum inflammatory factors (CRP, TNF-α and IL-17) were lower in the observation group than in the control group (all P < 0.05). The levels of NK, CD4⁺ and CD4⁺/CD8⁺ were higher in the observation group than in the control group (all P < 0.05). The IgG and IgM values were significantly lower in the observation group than those in the control group (all P < 0.05). Conclusion LEI's Yinzhan ginger-padded moxibustion combined with Buhuo Shengtu therapy has a definite effect on the treatment of UC in the remission stage, and its mechanism may be related to the regulation of intestinal inflammation and immune function.

Clinical study on treatment of acute gouty arthritis with cross needle knife combined with modified suction cupping

ZHANAG Sheng, WANG Hong-nan, ZHENG Zhou-xia

2022, 20(5): 848-850. doi: 10.16766/j.cnki.issn.1674-4152.002470

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Objective To explore the value of cross needle knife combined with improved suction cup in the treatment of acute gouty arthritis. Methods Sixty patients with acute gouty arthritis who were treated in Zhoushan Maternity and Child Health Hospital from January 2016 to December 2018 were randomly divided into the treatment and control groups, with 30 cases in each group. The treatment group was treated with cross-needle knife combined with modified suction cupping, with 2 times as a course of treatment. The control group was treated with diclofenac sodium sustained-release capsules, 50 mg/time, 3 times/day. Both groups were treated continuously for 14 days. The clinical effects of the two groups were compared. The visual analogue scoring method was used to compare the pain of the two groups before and after treatment, and the enzyme-linked immunosorbent method was used to measure the level of inflammatory factors in the patients. Results After treatment, the total clinical effective rate of the treatment group (96.67%) was higher than that of the control group (70.00%), and the difference was statistically significant (χ²=7.680, P=0.006). The VAS score of the treatment group after treatment was (1.03±0.43) points, lower than that of the control group [(3.38±1.01) points, t=11.726, P < 0.001]; CRP, IL-1β, IL-6 of the treatment groups after treatment were (6.23±1.36) mg/L, (3.37±0.58) pg/L, (6.56± 2.08) pg/L, lower than the control group [(11.41±3.21) mg/L, (5.57±1.01) pg/L, (9.87±1.74) pg/L; t=8.138, P < 0.001; t=10.346, P < 0.001; t=6.685, P < 0.001)]. Conclusion The cross-needle knife combined with the modified suction cupping exerts a significant clinical effect in the treatment of acute gouty arthritis, which can significantly relieve the pain of patients.

Action research on the establishment of seminar model for nursing graduate students

KANG Feng-ying, LI Wen-jing, GUO Min-min, CAO Wen-qian, YANG Yun-jing, LIU Xing-fang

2022, 20(5): 851-854. doi: 10.16766/j.cnki.issn.1674-4152.002471

365 4

Abstract:
Objective To construct a seminar model for nursing graduate students and verify its effect. Methods Seminars were organised for nursing graduate students from 2015 to 2017 by observing and reflecting the cycle of planned action. Through the research motivation scale (RMS), nursing research self-efficacy scale (NURSES), and scientific research ability self-evaluation rating scales of nursing staff (SRASES), we collected data and identified problems to improve the teaching model. Results (1) Continuous improvement and construction of seminar mode with curriculum, content, and classroom organisation as the framework was achieved. (2) The total score of RMS and the scores of each dimension increased yearly, and the difference was statistically significant (all P < 0.05). The total score of NURSES and the scores of "access to scientific knowledge resources", "collective research efficiency" and "theoretical research and application" increased year by year. The scores of "critical reading of quantitative literature", "critical reading of qualitative literature" and "collective research efficiency" increased from the first year, and the scores of "access to scientific knowledge resources" and "theoretical research and application" increased from the second and third years, with statistically significant differences (all P < 0.05). The total score of SRASES and the scores of all dimensions increased from the second year and subsequently increased yearly (all P < 0.05). Conclusion The newly established seminar model show actual effect on the improvement of nursing graduate students' scientific research motivation, scientific research efficacy and scientific research ability.

Application of case-based learning mode combined with stage goal teaching method in the practice of infectious diseases

HU Wen-long, CHEN Ren-qiong, ZHU Zheng-yun, ZHANG Ting-ting

2022, 20(5): 855-858. doi: 10.16766/j.cnki.issn.1674-4152.002472

192 4

Abstract:
Objective To observe the application of case-based learning(CBL) combined with stage goal teaching method in the practice of infectious diseases. Methods A total of 160 students who participated in the practice of infectious diseases in a third-class hospital from July 2018 to July 2019 were selected as the research objects. All students were randomly divided into two groups by random digital table method: the observation group (n=80, case-based learning combined with stage goal teaching method) and the control group (n=80, traditional teaching method). The scores of theoretical examination, case analysis and clinical skills of the two groups were compared, and the teaching satisfaction of the observation group was collected. Results The scores in the entrance theory test of the observation group and the control group were similar [(86.66±4.57) points and (87.50±1.29) points, respectively], with no significant difference (t=-1.576, P=0.118). After the practice, the theoretical scores of the observation group were slightly higher [(36.25±1.71) points] than the control group [(32.50±3.11) points], the difference was significant (t=9.338, P < 0.001). The scores of case analysis ability [(25.23±1.82) points] and clinical operation examination [(28.50±2.16) points] in the observation group were significantly higher than those in the control group [(19.50±3.44) points and (24.65±4.08) points, t=13.150, 7.458, all P < 0.001]. The questionnaire survey of the observation group showed that this teaching method helped to improve learning enthusiasm, clinical thinking and practical application ability. Conclusion CBL combined with stage goal teaching method can improve the case analysis and clinical operation ability, and improve the mastery of specialised knowledge of infectious diseases. Students are highly satisfied with teaching, and most hope to continue to use this teaching method.

Construction and application of online open course of occupational health and occupational medicine based on small private online course model

ZOU Xue-min, ZHU Le-mei, LI Dong-yang, ZENG Ling-feng, LIU Juan

2022, 20(5): 859-863. doi: 10.16766/j.cnki.issn.1674-4152.002473

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Abstract:
Objective To explore a new teaching mode suitable for the course through the construction and application of the online open course of occupational health and occupational medicine in small private online course (SPOC) mode, in order to provide paradigm and reference for the research of teaching reform. Methods Grade 14 and 16 undergraduate classes of preventive medicine in Changsha Medical College were selected as the research object. The grade 16 class was selected as the experimental group using SPOC teaching mode, and the grade 14 class was the control group using routine offline teaching. Relying on the Superstar learning platform, an online open course of occupational health and occupational medicine was built. It had carried out reform and exploration in the aspects of curriculum content structure, teaching methods, teaching process and assessment mechanism, and carried out differentiated teaching. The teaching effect of this teaching method was evaluated through questionnaire survey and course examination results. Results Through the teaching reform, the online resources of online open courses of occupational health and occupational medicine were more abundant and perfect. 89.89% of the students in grade 16 class were satisfied with the overall evaluation of SPOC mode teaching method, 92.13% of the students thought that they had improved their autonomous learning ability, 84.27% of the students were satisfied with the interaction between your teachers and students, 87.64% of the students felt that the teaching method provided rich teaching resources. The ability of teamwork, improving language expression and consulting literature had been improved to varying degrees. The scores of experimental operations, theory and total scores of grade 16 class were higher than those of grade 14 class, and the difference was statistically significant (all P < 0.05). Conclusion SPOC teaching mode can fully mobilize students' enthusiasm and initiative, it can improve students' mastery of knowledge points in some extent. It can significantly improve students' learning effect and good students' satisfaction, which can provide an effective reference for online teaching.

Deficiencies and coping strategies of network teaching for undergraduates in medical colleges in the new era

WANG Qiang, CHEN Wei-dong, GAO Yong

2022, 20(5): 864-866. doi: 10.16766/j.cnki.issn.1674-4152.002474

232 8

Abstract:
With the popularisation and development of computer and network information, a new teaching method termed network teaching has emerged. The goal of developing online education in China is inseparable from the promotion of educational informatisation in China. As early as 1998, the Ministry of Education began to launch the 'action plan for the revitalisation of education for the 21st century', thereby promoting the rapid development of modern distance education in China. In the 2000 national conference on education, the State Council and the 'deepening the reform of education comprehensively promote quality education', mentioned in the decision to vigorously improve the level of modernisation of education technology and education informatisation level, national support construction in Chinese education research network and satellite video system on the basis of the modern distance education network. In 1998, the Ministry of Education approved Tsinghua University, Zhejiang University, Beijing University of Posts and Telecommunications and Hunan University to hold modern distance education pilot programmes. Network teaching has become one of the teaching methods in various universities, and it has also been strongly advocated and supported by the national education department. The development of network teaching has accelerated the speed of education reform, integrated and optimised education resources and allowed education keep pace with the times. It provides a new idea for promoting educational fairness and justice. Traditional medical teaching makes boring knowledge difficult to understand, and the teaching effects are not ideal. Medical network teaching may be better than traditional modes to impart medical knowledge. Therefore, in accordance with the teaching features of the medical profession, this paper introduces the basic information of online teaching in current medical colleges, discusses the advantages of online teaching, illustrates the necessity and significance of online teaching at medical colleges and universities and existing problems and proposes solutions to conduct a comprehensive analysis to actively improve the online teaching situation. This work hopes to provide scientific reference for the advancement and development of medical online teaching.

Analysis on influencing factors of serum transaminase of medical staff based on physical examination in a hospital

WANG Jing, CAO Da-long, WU Xue-sen, MI Jing

2022, 20(5): 867-871. doi: 10.16766/j.cnki.issn.1674-4152.002475

251 2

Abstract:
Objective To understand the abnormal detection and its influencing factors of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) among medical staff in a tertiary general hospital based on physical examination data during 8 years for providing evidence in making prospective, targeted and personalized health management programs. Methods Medical Physical examination data of 6 074 staffs in a tertiary general hospital from 2012 to 2019 were collected and analyzed. Univariate analysis was conducted to analyze the influencing factors of ALT and AST, and further variables with statistical significance were included in logistic regression analysis. Results The abnormal detection rate of ALT was 10.96%, which was significantly higher in male than that in female (68.47% vs. 31.53%). The abnormal detection rate of AST was 2.49%, which was significantly higher in male than that in female (60.26% vs. 39.74%). Logistic regression analysis showed that female was the protective factor for ALT, with the OR(95% CI) of 0.273 (0.221-0.331), while higher DBP, higher UA and higher RBC were the risk factors for ALT, with the OR(95% CI) of 1.455 (1.126-1.882), 1.516 (1.177-1.952) and 1.884 (1.325-2.680). Older age and higher UA were the risk factors for abnormal AST, with the OR(95% CI) of 1.626 (1.116-2.369) and 2.013 (1.371-2.957). Conclusion The status of liver function of medical staff is not optimistic. It is necessary to arouse the consciousness of loving liver and protecting liver. Medical staff should take regular physical examination, pay attention to the results of physical examination, change life attitude, life style and life habits, and maintain physical and mental health.

Correlation between body image and posttraumatic growth in patients with head and neck tumour after surgery

LIU Lu-lu, LI Xiu-chuan, LI Jian-cheng, JI Hong-min, JIANG Jie

2022, 20(5): 872-876. doi: 10.16766/j.cnki.issn.1674-4152.002476

280 2

Abstract:
Objective To investigate the relationship between body image and posttraumatic growth in patients with head and neck tumours. Methods A total of 206 cases of postoperative head and neck tumour who were hospitalised or out-patients in a third-class A hospital in Anhui Province from September 2020 to March 2021 were selected as the research objects. The self-compiled general data questionnaire, Body Image Scale and C-Posttraumatic Growth Inventory were used to investigate the subjects. Results The total BIS score of patients with head and neck cancer after surgery was (7.12±3.56) points, 61 cases with total BIS score ≥10 and 145 cases with total BIS score < 10. The incidence of body image disorder was 29.6%. The total score of C-PTGI was (60.91±9.74) points. The cut-off point is divided by 10, the score of C-PTGI and the score of all dimensions in patients with BIS score ≥10 were higher than those in patients with BIS score < 10 (all P < 0.05). Pearson correlation analysis showed that the BIS total score and each dimension score of patients with postoperative head and neck cancer were negatively correlated with C-PTGI total score and each dimension score, and the difference was statistically significant (all P < 0.05). The results of the hierarchical regression analysis model showed that body image was the influencing factor of posttraumatic growth after controlling for general data (β=-0.513, ΔR²=0.222, F=41.147, P < 0.001). Conclusion Both body image and post-traumatic growth of patients with head and neck cancer after surgery need to be improved. Good body image can promote post-traumatic growth, and clinical medical staff can improve patients' post-traumatic growth from the perspective of improving body image.

Relationship between parental rearing patterns and children's Internet gaming disorder in the context of COVID-19

CHEN Hong-xu, LI Xin-yi, LI Yi-ting, XU Zhen-dong, MU Ze-yue, JIAO Dong-liang

2022, 20(5): 877-881. doi: 10.16766/j.cnki.issn.1674-4152.002477

319 5

Abstract:
Objective To investigate the relationship between parental rearing patterns and children's Internet game addiction in the context of COVID-19. Methods The survey was conducted from 6 August 2020 to 27 August 2020. The research subjects were divided into two parts: one part was children, who were college students from freshman to fifth year in Bengbu Medical College. The second part was parents, and the parents' questionnaire was sent to their parents by their children. The questionnaire for children was conducted by random sampling of the whole class. Parental Bonding Instrument (PBI) and Online Game Addiction Questionnaire were used to investigate the relationship between parenting style and the online game addiction, and the Online Game Cognition Questionnaire was used to investigate the cognitive status of parents and children on online game addiction amongst college students. Results During the epidemic, online games were the main online entertainment activities for college students, and 42% of them considered that their online gaming behaviours had increased compared with those before the epidemic. The higher the degree of parental care and control, the higher the total score of children's online game addiction; the difference was statistically significant (P < 0.01). The total score of online game addiction on the group of authoritarian, despotism and permissiveness was significantly higher than that of permissiveness and ordinary ones in parents' parenting style (authoritarian P < 0.001, despotism, and permissiveness P < 0.05). Parents and children had different perceptions of the use, guidance and addiction of their children's online games (all P < 0.05). Conclusion There is a positive correlation between parents' high care and high control and children's online game addiction. The reason may be the difference between parents and children in understanding their children's use of online games, guidance and addiction. Therefore, when guiding children to use online games correctly, parents need to objectively understand online games and communicate with their children to find acceptable and effective methods, which may help improve parent-child relationships and alleviate the children's online game addiction problems.

Effect of improved continuous nursing on surgical stress and immune function in patients with gastric cancer after laparoscopic distal gastrectomy

TANG Ting, ZHANG Qiang, WANG Xiang, HE Li-yun, ZHANG Wen-bin

2022, 20(5): 882-885. doi: 10.16766/j.cnki.issn.1674-4152.002478

266 2

Abstract:
Objective To analyze the impact of improved continuous nursing based on the concept of accelerated rehabilitation surgery on the surgical stress and immune function of gastric cancer patients after laparoscopic distal gastrectomy. Methods From June 2018 to June 2020, 98 patients with gastric cancer who underwent laparoscopic distal gastrectomy were selected from the First Affiliated Hospital of Xinjiang Medical University. They were divided into the observation group (49 cases) and control group (49 cases) according to the random number table method. The control group was given conventional nursing interventions, whereas the observation group was given improved continuous nursing based on the concept of accelerated rehabilitation surgery. Changes of stress function indexes at different stages and immune function indexes of both groups were compared after surgery. Results The levels of inflammatory mediators (IL-6, IL-8 and IL-10) of both groups on the 1st, 4th and 7th day after the operation increased compared with those before the operation, and the levels of IL-6 and IL-8 of the observation group were significantly lower than those of the control group (all P < 0.05). The two groups of patients had a decrease in CD4⁺, CD8⁺ and CD4⁺/CD8⁺ levels on the 1st and 4 th day after operation, while CD4⁺ increased on the 7th day after operation (all P < 0.05), and there was no significant difference between the two groups (P > 0.05). The levels of IgA, IgG and IgM on the 1st and 4th days decreased, and the difference between groups was statistically significant (all P < 0.05). On the 7th day, the levels of IgA, IgG and IgM basically returned to the preoperative level. The levels of IgA and IgG of the observation group on days 1, 4 and 7 were higher than those of the control group, and the difference between groups was statistically significant (all P < 0.05). Conclusion The improved continuous nursing based on the concept of accelerated rehabilitation surgery can effectively improve the clinical effect of gastric cancer patients undergoing laparoscopic distal gastrectomy, reduce their stress inflammatory response, promote the recovery and improvement of their immune function to a certain extent and promote their recovery after surgery.

Application and effects of four-hand operation combined with knowledge-attitude-belief practice health education in root canal therapy

DING Jing, CHENG Chun, ZHANG Yang-yang, ZOU Ying

2022, 20(5): 886-889. doi: 10.16766/j.cnki.issn.1674-4152.002479

196 2

Abstract:
Objective To explore the application and effects of four-hand operation combined with knowledge-attitude-belief practice (KABP) health education in root canal therapy. Methods A total of 100 patients who underwent root canal therapy at the Endodontics Department of our hospital from January 2019 to January 2020 were enrolled as the research objects. They were divided into the observation group (n=50) and control group (n=50) by random number table method. The control group was given traditional operation nursing combined with routine health education, whereas observation group was given four-hand operation nursing combined with KABP health education for intervention. The time of root canal therapy, score of offending teeth visual analogue scale (VAS) after surgery, incidence of swelling, scores of Hamilton Anxiety Scale (HAMA), Hamilton Depression Scale (HAMD) and exercise of self-care agency scale (ESCA) before and after intervention and follow-up situations were compared between the two groups. Results After intervention, root canal treatment therapy, score of postoperative VAS, incidence of postoperative swelling, and scores of HAMA and HAMD in the observation group were all lower than those in the control group[(26.45±5.73) points, (3.36±1.12) points, 4.00%, (13.13±2.46) points, (7.29±1.45) points vs. (34.69±6.24) points, (4.28±1.34) points, 16.00%, (15.47±2.31) points, (8.53±2.06) points, all P < 0.05]. The total score of ESCA in the observation group was higher than that in the control group[(125.82±6.53) points vs. (112.03±7.55) points, P < 0.05]. During follow-up, the incidence of temporary objects shedding in the observation group was lower than that in the control group (8.00% vs. 24.00%, P < 0.05), whereas the proportions of patients undergoing regular subsequent visit and full crown restoration were significantly higher than those in the control group (92.00%, 78.00% vs. 74.00%, 58.00%, all P < 0.05). Conclusion The application of four-hand operation combined with KABP health education for nursing intervention in patients undergoing root canal therapy can significantly shorten surgical treatment time, improve psychological status, alleviate postoperative pain and improve self-care ability and the incidence of regular subsequent visit.

The moderating role of career resilience on the relationship between workload and emotional exhaustion

TANG Xiao-li, CHEN Li-min, DONG Fen, XU Jin-liang

2022, 20(5): 890-893. doi: 10.16766/j.cnki.issn.1674-4152.002480

252 4

Abstract:
Objective Career resilience is an increasingly important but under-researched aspect of modern careers, especially amongst nurses. To improve nurses' career resilience and relieve emotional exhaustion, this study aimed to investigate the current situation of workload and emotional exhaustion and explore whether career resilience moderates the effect of workload on emotional exhaustion. Methods Using convenience sampling methods, 466 nurses enrolled in ten public hospitals in Zhejiang Province were selected as subjects from August to October in 2020. They were investigated by workload scale, career resilience scale and work emotional exhaustion scale. Data were analysed using SPSS 20.0 and AMOS 13.0. Results The scores of workload, career resilience and emotional exhaustion of nurses were (3.73±0.52) points, (3.44±0.76) points and (3.60±0.49) points, respectively. The scores of workload and work emotional exhaustion were statistically significant for gender, working years and positional titles. Correlation analyses showed that workload was negatively associated with career resilience (r=-0.362, P < 0.001) but positively associated with emotional exhaustion (r=0.476, P < 0.001). Career resilience was negatively related to emotional exhaustion (r=-0.306, P < 0.001). Multiple regression analysis and sample slope analysis showed that career resilience moderated the relationship between workload and emotional exhaustion. Conclusion Workload is an important factor affecting nurses' emotional exhaustion. High career resilience can relieve the positive effect of workload on emotional exhaustion.

2022, 20(5): 894-896. doi: 10.16766/j.cnki.issn.1674-4152.002481

225 22

Abstract:

2022, 20(5): 897-899. doi: 10.16766/j.cnki.issn.1674-4152.002482

247 9

Abstract:

2022, 20(5): 900-902. doi: 10.16766/j.cnki.issn.1674-4152.002483

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Mechanism of Shenfu Decoction in the treatment of atrial fibrillation based on network pharmacology and molecular docking

SHI Wei-hua, CHAO Xiao-ying, SU Yong-xing, LI Shuo, LI Xiao-li, ZHONG Guo-qiang

2022, 20(5): 727-730. doi: 10.16766/j.cnki.issn.1674-4152.002440

383 15

Abstract:
Objective To explore the mechanism of Shenfu Decoction in the treatment of atrial fibrillation by network pharmacology and molecular docking technology. Methods The main active ingredients of Shenfu Decoction were screened by the TCMSP database, and the related targets were annotated by the Uniprot database and gene names were obtained. The targets of atrial fibrillation were obtained from the OMIM and Pharmgkb Databases. R software was used to determine the intersection of atrial fibrillation and the active ingredient targets of Shenfu Decoction, and the therapeutic targets were obtained. The PPI network was constructed by using the online String database, and the results were imported into Cytoscape software to construct the "active ingredient-target-disease" network and perform topology analysis to obtain the core targets. The GO and KEGG enrichment analyses of key targets in the DAVID database were carried out, and AutoDock Vina software was applied to conduct molecular docking of key components and key targets. Results The 101 targets of Shenfu Decoction and 3 219 targets of atrial fibrillation were obtained, and 65 common targets were obtained after the intersection. Topology analysis showed that NFKBIA, RELA, IL1B, JUN, STAT1 and MAPK8 might be the core targets of Shenfu Decoction in treating AF. GO suggested that the targets involved biological processes such as voltage-gated calcium channel activity, calcium ion transport and reactive oxygen metabolism. KEGG suggested that the targets were involved in the treatment of atrial fibrillation through cGMP-PKG, cAMP, calcium channel and PI3K-Akt signalling pathways. The results of molecular docking showed that the core target protein had good binding stability with the corresponding active components. Conclusion The treatment of atrial fibrillation with Shenfu Decoction has the characteristics of "multi-component, multi-target and multi-pathway", which may be related to targets of NFKBIA, RELA, IL1B, JUN, STAT1 and MAPK8. The specific mechanisms may need to be confirmed by molecular biological experiments.

Analysis of risk factors for the prognosis of IgA nephropathy

FAN Jing, HUANG Guan-wen, BAO Ji-wen, ZHANG Min-fang, ZHOU Wen-yan, WANG Qin, NI Zhao-hui, WANG Ling

2022, 20(5): 731-734. doi: 10.16766/j.cnki.issn.1674-4152.002441

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Objective To explore the risk factors affecting the prognosis of patients with IgA nephropathy by analyzing the clinical and pathological data of patients with IgA nephropathy, so as to provide clinical reference, and pay attention to early diagnosis and long-term follow-up. Methods The clinical and pathological data of patients with IgA nephropathy who underwent renal biopsy in Renji Hospital from December 2012 to December 2015 were analyzed. Serum creatinine (SCr) and 24-hour urinary protein were recorded every 3 months. The end points were set as SCr increased over 50% or reached end stage renal disease (ESRD) while following up. Kaplan Meier survival analysis was used to analyze the renal survival rate of patients with IgA nephropathy. Cox regression was used to analyze the prognostic risk factors of IgA nephropathy. Results Total 163 IgA nephropathy patients who had long term follow up (median follow up period 29 months) were included. The median age was 33 years old, 82(50.3%) were male, 7 patients (4.3%) reached the end points and 4-year kidney survival rate was 86.8%. The results of multivariate analysis of Cox proportional risk model showed that baseline systolic blood pressure (HR= 1.073, 95% CI: 1.018 - 1.132, P=0.009) and time averaged urinary protein excretion (TA-UPE, HR=2.123, 95% CI: 1.367-3.329, P=0.001) were the independent risk factors for prognosis of IgA nephropathy. Conclusion Various factors are correlated to the prognosis of IgA nephropathy whereas baseline systolic blood pressure and TA-UPE are the independent risk factors.

Application of MIMICS software three-dimensional reconstruction in complex segmental resection of lung adenocarcinoma

SONG Ming-lei, CAO Fu-min, XING Xiao-ying, GAO Li-ping, JING Hong-jia

2022, 20(5): 735-739. doi: 10.16766/j.cnki.issn.1674-4152.002442

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Objective To evaluate the clinical value of Materialise's interactive medical image control system (MIMICS) software 3D reconstruction combined with thoracoscopy in complex segmental resection of lung adenocarcinoma. Methods The control group comprised 42 patients who underwent thoracoscopic segmentectomy in our hospital from January 2016 to June 2017. The observation group was composed of 48 patients who underwent MIMICS software 3D reconstruction combined with thoracoscopic segmentectomy in the Fourth Hospital of Hebei Medical University from July 2017 to July 2018. The perioperative clinical parameters and operative complications were compared between the two groups. The pulmonary function indexes before operation and after removal of thoracic drainage tube were compared, including forced expiratory volume in one second (FEV₁), forced vital capacity (FVC) and maximum voluntary ventilation (MVV) per minute. Results No significant difference was found in the number of lesions and the nature of nodules between the two groups (P>0.05). The incidence of complications in the observation group (10.42%, 5/48) was significantly lower than that in the control group (28.57%, 12/42, P < 0.05). The average operation time, average intraoperative bleeding volume, average post-operative thoracic tube indwelling time and average post-operative hospital stay in the observation group were significantly shorter than those in the control group (all P < 0.05). There was no significant difference in FEV₁, FVC and MVV between the two groups before the operation (all P>0.05). After removal of the thoracic drainage tube, the pulmonary function indexes of the two groups were significantly lower than those before the operation. The FEV₁, FVC and MVV of the observation group were significantly higher than those of the control group [(1.67±0.52) L vs. (1.38±0.69) L, (1.73±0.64) L vs. (1.48±0.51) L, (54.27±7.14) L/min vs. (50.36±6.08) L/min, all P < 0.05)]. Conclusion For patients with pulmonary adenocarcinoma undergoing complex segmental resection, using MIMICS software 3D reconstruction before operation can help distinguish the anatomical structure of the lungs, determine the location of the nodules, improve the accuracy of the operation, shorten the operation time, reduce complications, reduce the damage of the lung function caused by the operation and facilitate rehabilitation after the operation.

Expression of TACC3 and its relationship with epithelial mesenchymal transformation in gastric adenocarcinoma

CAO Gao-xiang, LI Yi-jie, SHI Yi, SONG Guo-lei, JIANG Cong-qiao

2022, 20(5): 740-744. doi: 10.16766/j.cnki.issn.1674-4152.002443

289 4

Abstract:
Objective To investigate the expression of transforming acidic coiled-coil protein-3 (TACC3) in gastric adenocarcinoma (GAC) and explore the relationship between the expressions of TACC3 and epithelial-mesenchymal transition (EMT) and the clinicopathological parameters of patients. Methods A total of 113 GAC specimens and corresponding matched normal tissue specimens filed in Departments of Pathology, the First Affiliated Hospital of Bengbu Medical College from October 2013 to October 2014 were analysed for TACC3, E-cadherin and vimentin protein expressions by immunohistochemical staining. Results The positive expression rates of TACC3, E-cadherin and vimentin in GAC specimens and normal gastric tissues were 67.3%, 45.1%, 50.4% and 17.7%, 69.9%, 27.4%, respectively. The difference was statistically significant. Kaplan-Meier survival analysis showed that the survival time of patients with positive expression of TACC3 and vimentin was lower than that of patients with negative expression, and the difference was statistically significant (all P < 0.001). Patients who had positive expression of E-cadherin had a significantly higher OST than those with negative expression (P < 0.001). Multivariate analysis demonstrated that the positive expression of TACC3 and vimentin and the negative expression of E-cadherin were independent prognostic factors for GAC (all P < 0.05). Spearman correlation coefficient analysis showed that the expressions of TACC3 and vimentin were positively related. The relationship between E-cadherin and TACC3 was negatively correlated. The relationship between E-cadherin and vimentin was negatively correlated (all P < 0.001). Conclusion High expression of TACC3 is found to be related to EMT and poor prognosis in GAC. TACC3 is expected to become a new target for GAC targeted therapy, and early detection of TACC3 expression can be used to evaluate the metastasis and prognosis of GAC patients.

Mechanism of miRNA-19a targeting the SMO gene regulates the hedgehog signalling pathway and affects the biological characteristics of myeloma cells

LI Guang-bao, YIN Jian-wen, ZHANG Yi-qun, LI Shi-zhong, WU Zu-tong, HE Ai-jun

2022, 20(5): 745-748. doi: 10.16766/j.cnki.issn.1674-4152.002444

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Objective To explore the effects of miRNA-19a on the proliferation, apoptosis and metastasis of multiple myeloma cells, as well as on smoothened frizzled class receptor(SMO) protein and the hedgehog signalling pathway. Methods Human myeloma cell lines U266b1, LP-1, RPMI 8226 and NCI-H929 were used as the research models. Firstly, the background expression of SMO protein in four kinds of cells was detected. Using U266b1 cells as the model, the miRNA-19a inhibitor was designed, and the miRNA-19a knockdown group, control plasmid group and empty group were established. Changes in the SMO protein content, cell proliferation, apoptosis and metastasis were explored after miRNA-19a knockdown. Cytotoxicity was detected by MTT assay, apoptosis was detected by flow cytometry and cell invasion was detected by Transwell test. Results The expression levels of SMO protein in the U266b1, LP-1, RPMI 8226 and NCI-H929 cell lines were 0.925±0.057, 0.889±0.067, 0.904±0.075, and 0.507±0.048, respectively, whereas the expression level of SMO protein in the NCI-H929 cell line was low (P < 0.05). The miRNA-19a contents in the miRNA-19a knockdown group, control plasmid group and empty group were 0.325±0.058, 1.158±0.128 and 1.172±0.131, respectively. The miRNA-19a contents in the miRNA-19a knockdown group decreased significantly (P < 0.05). The expression levels of SMO in the control plasmid group, miRNA-19a knockdown group and empty group were 0.787±0.104, 0.354±0.068 and 0.807±0.106, respectively. The content of SMO protein in the miRNA-19a knockdown group decreased significantly (P < 0.05). MTT assay showed that the OD value of cell proliferation in the miRNA-19a knockdown group was significantly lower than that in the empty group and control plasmid group within 24-72 h (all P < 0.05). Apoptosis experiments showed that the apoptosis rate of the miRNA-19a knockdown group was significantly higher than that of the empty group and control plasmid group (all P < 0.05). Transwell experiments showed that the number of invasive cells in the miRNA-19a knockdown group was significantly lower than that in the two other groups (all P < 0.05). Conclusion miRNA-19a/SMO protein may be the key signal to control the malignant growth of cells in myeloma cell line U266b1. It can be used as a potential target for the treatment of multiple myelomas.

Clinical diagnosis and treatment of inferior vena cava variant complicated with lower extremity deep vein thrombosis

WANG Chen-meng, YU Chao-wen, NIE Zhong-lin, LU Ran, GUAN Ze-yu, WANG Xiao-gao

2022, 20(5): 749-751. doi: 10.16766/j.cnki.issn.1674-4152.002445

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Objective To investigate the clinical diagnosis and treatment of congenital inferior vena cava malformation (IVCM) complicated with deep vein thrombosis (DVT). Methods The clinical data of 16 patients with inferior vena cava variation treated in Department of Vascular Surgery, the First Affiliated Hospital of Bengbu Medical College from August 2011 to July 2021 were analysed retrospectively. Results Amongst the 12 cases of double inferior vena cava (DIVC), 9 cases were complicated with lower extremity DVT (5 cases with left lower extremity DVT underwent inferior vena cava filter implantation plus catheter thrombolysis; 3 cases complicated with right lower extremity DVT underwent right IVC filter implantation; and 1 case complicated with both lower extremity DVT was inserted with 1 filter in the left and right IVC, respectively), and 3 cases complicated with unexplained lower extremity swelling were treated with detumescence. Four cases were diagnosed with DVT in the left inferior vena cava. Amongst them, 2 cases found by left lower extremity deep venography due to lower extremity swelling were given detumescence treatment; 2 cases found by left femoral vein puncture during right lower extremity DVT were implanted with an inferior vena cava filter, of which 1 case was treated by catheter thrombolysis at the same time. There were 1 case of permanent filter and 10 cases of recyclable filter. All patients with DVT were given anticoagulant therapy after operation. Conclusion IVCM is an important risk factor of venous thromboembolism, and the placement of inferior vena cava filter in an appropriate position has important clinical significance.

Effect of cardiac sympathetic nerve on the heart of spontaneously hypertensive rats

YUAN Hang, CHU Wan-fei, XU Qiang

2022, 20(5): 752-755. doi: 10.16766/j.cnki.issn.1674-4152.002446

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Abstract:
Objective To investigate the effect of cardiac sympathetic nerve on the heart of spontaneously hypertensive rats. Methods Thirty spontaneously hypertensive male rats aged 6-7 weeks were selected and divided into the observation group and control group according to the random number table method, each with 15 rats. The rats in the observation group were treated with sympathetic ganglion resection, and the sympathetic ganglion in the neck of the rats in the control group was exposed. The heart rate, blood pressure, left ventricular diastole, end-systolic diameter, left ventricular ejection fraction (LVEF) and myocardial collagen tissue Ⅰ, myocardial collagen tissue Ⅲ, connective tissue growth factor and norepinephrine transporter mRNA expression levels were compared between the two groups of rats. Results The heart rate of the observation group was significantly lower than that of the control group [(374.48±17.46) times/min vs. (403.12±14.56) times/min, t=4.879, P < 0.05]; The left ventricular end-diastolic diameter and left ventricular end-systolic diameter of the observation group were significantly lower than those in the control group [(4.97±0.52) mm vs. (4.51±0.45) mm, (3.06±0.23) mm vs. (2.57±0.17) mm, t=2.591, 6.635, all P < 0.05], The LVEF of the observation group was significantly lower than that in the control group [(71.48±4.46)% vs. (80.12±3.56)%, t=5.864, P < 0.05]; The expression of NET mRNA in the observation group were significantly higher than that in the control group (1.507±0.054 vs. 1.424±0.046, t=4.532, P < 0.05), and the expression of myocardial collagen Ⅲ and CTGF mRNA was significantly lower than that in the control group (1.245±0.046 vs. 1.359±0.036, 1.434±0.095 vs. 1.541±0.012, t=7.559, 4.328, P < 0.05). Conclusion Cardiac sympathetic nerve can adjust the CTGF, NET, inhibit myocardial hypertrophy and myocardial fibrosis in spontaneously hypertensive rats.

Expression characteristics of tumor necrosis factor alpha inducible protein 3 in glioma and its effect on invasion and migration of glioma cell

WANG Chao-chao, CHENG Zhe, CHANG Xue-lian, WANG Bang, WU Juan, HE Shi-wei, WANG Da-wei

2022, 20(5): 756-760. doi: 10.16766/j.cnki.issn.1674-4152.002447

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Abstract:
Objective To investigate the expression of tumor necrosis factor alpha inducible protein 3 (TNFAIP3) in gliomas and its effect on the invasion and migration of glioma cells. Methods Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of TNFAIP3 in normal human astrocytes NHA and glioma cell lines U87, U251, SNB19, T98 and LN229, respectively. U87 and SNB19 were used as experiments subjects. Lipofectamine 2000-mediated small interference RNA (siRNA) was used to transfect U87 and SNB19 to down-regulate the expression of TNFAIP3. The effect of down-regulated TNFAIP3 on the invasion of U87 and SNB19 was detected by Transwell, and the migration was detected by scratch test. Results Compared with NHA (0.144±0.008), the expression of TNFAIP3 in U87 (1.380±0.066), U251 (0.663±0.062), SNB19 (1.405±0.032), T98 (1.002±0.068) and LN229 (0.667±0.027) were increased (all P < 0.05). Si-TNFAIP3 transfection into U87 and SNB19 could down-regulate the expression of TNFAIP3 and continue to culture for 24 hours, the invasion and migration ability of U87 and SNB19 were decreased (all P < 0.05). Conclusion TNFAIP3 is highly expressed in glioma, which can promote the invasion and migration of U87 and SNB19, and si-TNFAIP3 can inhibit the invasion and migration of glioma cells.

Effect of salidroside on the proliferation and apoptosis of testicular spermatogenic cells in infertile rats

ZHANG Chang-yin, TAO Zhong-jie, ZHU Chen-jie

2022, 20(5): 761-765. doi: 10.16766/j.cnki.issn.1674-4152.002448

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Objective To investigate the effect of salidroside on the proliferation and apoptosis of spermatogenic cells in infertile rats. Methods Fifteen rats were Selected from 60 rats as group A, and 45 infertility model rats were randomly divided into groups B, C and D, with 15 rats in each group. Groups C and D were given 50 and 100 mg/kg of salidroside via gavage, and groups A and B were given the same amount of normal saline. Serum testosterone, follicle-stimulating hormone, and luteinising hormone were detected. Superoxide dismutase (SOD) and malondialdehyde (MDA) were detected by enzyme-linked immunosorbent assay. Haematoxylin and eosin (HE) staining was used to detect the morphology of spermatogenic cells. Proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry, and the apoptosis rate of spermatogenic cells was detected by TUNEL assay. Western blotting was used to detect the relative expression of fatty acid synthase (Fas), fatty acid synthase ligand (Fasl) and caspase-3. Results Compared with that in group A, the SOD levels in groups B, C and D were decreased, and the order was follows: group B < group C < group D (all P < 0.05). Compared with those in group A, the relative expression levels of MDA, Fas, Fasl, and caspase-3 protein in groups B, C and D were increased, and the order was as follows: group B>group C>group D (all P < 0.05). Compared with group B, the structure of testicular cells, atrophy of seminiferous tubules, and exfoliation of spermatogenic cells in group D were significantly improved. The percentage of PCNA in spermatogenic cells of group A was (41.23±5.46)%, which was higher than that in group B (20.13±3.21)%, group C [(27.64±3.25)%] and group D [(35.11±4.56)%], all P < 0.05. The apoptosis rate of spermatogenic cells in group A was (2.23±0.03)%, which was lower than that in group B [(10.03±1.12)%], group C [(7.94±0.89)%] and group D [(4.07±0.52)%], all P < 0.05. Conclusion Salidroside can regulate the proliferation and apoptosis of spermatogenic cells in infertile rats. The mechanism may be related to the downregulation of the Fas/Fasl signalling pathway.

Safety and efficacy of intravenous tirofiban after endovascular treatment of acute cerebral infarction

HUANG Jie, LUO Song, LI Jin-ping, LI Xiao-xiong, LIU Cheng-chun, FU Xiao-yan, ZHOU Hua-dong

2022, 20(5): 766-769. doi: 10.16766/j.cnki.issn.1674-4152.002449

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Objective To evaluate the safety and efficacy of intravenous tirofiban after endovascular treatment for patients with acute cerebral infarction. Methods A total of 152 patients with acute cerebral infarction undergoing endovascular treatment in the Department of Neurology of Daping Hospital, Army Military Medical University from September 2019 to March 2021 were retrospectively included. They were divided into the tirofiban group and control group according to whether tirofiban was injected intravenously after endovascular treatment. The baseline of the two groups was balanced by 1∶ 1 propensity score matching analysis. The main end points included safety and effectiveness. The safety was symptomatic intracranial haemorrhage (sICH), any haemorrhage, recurrence within 90 days and mortality. The effectiveness was National Institutes of Health Stroke Scale (NIHSS) score at discharge and 90-day good prognosis rate (modified Rankin score: 0-2). Results A total of 145 patients were included, including 70 in the tirofiban group and 75 in the control group. The NIHSS score of the tirofiban group at discharge [4(2, 13) vs. 11(3, 16), P=0.002] was significantly better than that of the control group. Moreover, there was no significant difference in the incidence of sICH [7(10.0%) vs. 12(16.0%), P=0.284] 90-day prognosis, any bleeding, recurrence and mortality (all P>0.05). Forty-seven pairs were successfully matched by propensity score. There was no significant difference in safety and effectiveness between the two groups (all P>0.05). Conclusion Intravenous tirofiban is safe after endovascular treatment of acute cerebral infarction. Pre-matching tirofiban can improve the NIHSS score at discharge of patients with acute cerebral infarction after endovascular treatment, but it has no benefit in the 90-day prognosis rate. After matching, there was no benefit in the good prognosis of tirofiban patients at discharge and 90 days.

Observation on the effect of complete laparoscopic bladder flap plasty for full-length ureteral avulsion

WEI Hai-bin, BAI Yu-chen, MAO Zu-jie, ZHANG Qi, LI En-hui, QI Xiao-long, LIU Feng, ZHANG Da-hong

2022, 20(5): 770-772. doi: 10.16766/j.cnki.issn.1674-4152.002450

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Objective To explore the feasibility and safety of laparoscopic bladder flap reconstruction and pedicled omentum coverage for full-length ureteral avulsion (from the junction of the renal pelvis and ureter to the end of the ureter). Methods The clinical data of 5 patients with full-length ureteral avulsion admitted to the Department of Urology, Zhejiang Provincial People's Hospital from November 2014 to August 2019, including 3 cases on the right side and 2 cases on the left side, were retrospectively analysed. All cases of ureteral avulsion occurred during the endoscopic lithotripsy in the other hospital. Three patients underwent one-stage emergency surgical repair. One patient had repeated replacement of the DJ tube and second-stage surgical repair 1 year later. Another patient underwent open surgery during the initial surgery to repair the ureter in situ. Second-stage surgical repair was performed 5 days later due to urine leakage and peritonitis. All patients underwent complete laparoscopic bladder flap formation and pedicled omentum wrapping surgery. Clinical data were collected and recorded during the perioperative and follow-up period. Results All repair and reconstruction operations were successfully completed. No urine extravasation, retroperitoneal infection and hydronephrosis occurred during hospitalisation. For the 3 patients undergoing emergency surgery, the DJ tube was replaced once and removed 6 months postoperatively. In the patient who underwent second-stage operation 5 days later, the DJ tube was changed 3 months postoperatively, and the patient was still in the state of intubation. In another patient, the DJ tube was changed repeatedly due to backache, and the patient underwent laparoscopic nephrectomy on the affected side 1 year after the operation. Except for the patient undergoing nephrectomy, no aggravated separation of the collection system and stenosis of the anastomosis occurred. Postoperative urination was smooth, and frequence and ureteral reflux were not obvious. The cystography was pear-shaped and biased to the affected side, and the volume was slightly smaller. Conclusion Complete laparoscopic bladder flap plasty and pedicled omental wrap repair surgery is a safe and effective minimally invasive treatment method for full-length ureteral defects.

Observation on the curative effect of replantation of autologous thick skin in the donor area of elderly medium-thickness skin

WANG Zhen-jun, PAN Sun-feng, FANG Gao-feng, LIU Liang, ZHU Min-da

2022, 20(5): 773-776. doi: 10.16766/j.cnki.issn.1674-4152.002451

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Objective To observe the therapeutic effect of autologous thick skin replantation in the donor area of medium-thickness skin in elderly patients and explore a new therapeutic method. Methods A total of 50 elderly patients with deep burns and scar contracture who were admitted to the Department of Burns and Orthopaedics of Jiaxing Hospital of Traditional Chinese Medicine from March 2019 to June 2020 and who met the inclusion criteria were selected and divided into the treatment group and the control group by random number table method, with 25 patients in each group. The anterolateral thigh was selected as the donor area in both groups, and medium-thickness skin slices (0.3-0.4 mm) were cut to repair burns, functional parts, scars and other wounds. In the treatment group, blade thick skin slices (0.12-0.15 mm) were cut beside the donor area of the medium-thickness skin slices, and the slices were perforated and transplanted into the donor area of the medium-thickness skin slices. The donor area of the control group was covered with Vaseline gauze and then bandaged under pressure. The healing time, Vancouver Scar Scale score and pruritus score of medium-thickness skin donor site were compared between the two groups. Results On the 7th day after operation, all the donor grafts in the treatment group survived, and the healing time of donor grafts in the treatment group [9.0(8.0, 12.0) d] was significantly shorter than that in the control group [21.0(20.5, 24.5) d], with statistical significance (P < 0.05). Three months after surgery, the Vancouver Scar Scale score in the treatment group [3.0(2.0, 3.5) points] was lower than that in the control group [10.0(9.0, 11.0) points], the difference was statistically significant (P < 0.05), and the pruritus score [1.0(1.0, 2.0) points] was lower than that in the control group [6.0(5.0, 7.0) points], and the difference was statistically significant all (P < 0.05). Six months after surgery, the Vancouver scar scale score in the donor area of the treatment group [1.0(0.5, 1.5) points] was lower than that of the control group [6.0(4.0, 8.5) points], the difference was statistically significant (P < 0.05), and the pruri- tus score [1.0(1.0, 1.0) points] was lower than that of the control group [2.0(1.0, 2.5) points], the difference was statistically significant (P < 0.05). Conclusion The use of autologous blade thick skin grafting medium-thickness skin donor area can significantly shorten the wound healing time, reduce the degree of scar hyperplasia and has a good clinical effect.

Changes and significance of red blood cell distribution width, blood uric acid, D-Dimer and B-ype natriuretic peptide in acute coronary syndrome

XUE Peng-cheng, LI Jing-jing, CHEN Kang-yu

2022, 20(5): 777-780. doi: 10.16766/j.cnki.issn.1674-4152.002452

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Objective To study the significance of red blood cell distribution width (RDW), blood uric acid (BUA), D-Dimer and B-type natriuretic peptide (BNP) levels in acute coronary syndrome (ACS). Methods Total 171 patients with ACS who underwent coronary angiography in the Third People's Hospital of Bengbu City from January 2017 to March 2019 were collected as experimental group, and 65 patients with no obvious abnormalities in coronary angiography or coronary CT during the same period were selected as controls group. The RDW, BUA, D-Dimer and BNP of the two groups were compared. The experimental group was divided into groups by Gensini score, and 4 indicators of each group were analyzed. The patients were grouped according to the adverse cardiovascular events (MACE) during hospitalization, and 4 indicators in each group were analyzed. Logistic regression was used to analyze the relationship between 4 indicators and ACS. Results The levels of RDW, BUA, D-Dimer and BNP in the experimental group were higher than those in the control group (all P < 0.01). Gensini scores were different in each group in RDW, D-Dimer and BNP (all P < 0.01). There were differences in 4 indicators in the MACE group (all P < 0.01). Logistic regression analysis showed that the 4 indicators were all related to ACS, the sensitivity were 0.468, 0.544, 0.655 and 0.404, the specificity were 0.738, 0.662, 0.708 and 0.754, and the area under the curve (AUC) were 0.610, 0.617, 0.670 and 0.584, respectively. The sensitivity of the combination factor composed of four indicators was 0.731, the specificity was 0.631, and the AUC was 0.714. Conclusion RDW, BUA, D-Dimer and BNP are related to the condition of ACS patients. The combined detection of 4 indicators has clinical significance in ACS.

Experimental study of miR-613 targeting PTBP1 to inhibit the invasion and migration of endometrial cancer cells

GU Shao-jun, QIAN Chun-yan, FU Huan-quan

2022, 20(5): 781-784. doi: 10.16766/j.cnki.issn.1674-4152.002453

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Objective To investigate the effects of miR-613 targeting polypyrimidine tract-binding protein 1 (PTBP1) on the proliferation, migration and invasion of endometrial cancer cells (Ishikawa). Methods Real-time fluorescent quantitative PCR (RT-qPCR) was conducted to detect the expression of miR-613 and PTBP1 mRNA in endometrial cancer tissues. Ishikawa cells were cultured in vitro, and intervened to overexpress miR-613, inhibit PTBP1 expression, or co-overexpress miR-613 and PTBP1. The proliferation, migration and invasion of Ishikawa cells and the protein expression levels of PTBP1, matrix metalloproteinase-2(MMP-2) and MMP-9 were detected respectively. Dual-luciferase reporter assay was conducted to verify the targeting relationship between miR-613 and PTBP1. Results The expression level of miR-613 in endometrial cancer tissue was 0.48±0.09, which was significantly lower than that in paracancerous tissue (1.03±0.11, P < 0.05). The expression level of PTBP1 mRNA was 2.78±0.23, which was significantly higher than that in paracancerous tissue (1.01±0.12, P < 0.05). MiR-613 was negatively correlated with PTBP1 mRNA (r=-0.523, P < 0.05). The results of dual-luciferase reporter gene assay showed that miR-613 targeted and negatively regulated the expression of PTBP1. Overexpression of miR-613 and inhibition of PTBP1 expression significantly decreased the survival rate of Ishikawa cells, the number of cell migration and invasion, and the expression levels of PTBP1, MMP-2 and MMP-9 (all P < 0.05). Overexpression of PTBP1 reversed the inhibitory effects of overexpression of miR-613 on Ishikawa cell proliferation, migration and invasion. Conclusion The expression of miR-613 is low in endometrial cancer tissues. Overexpression of miR-613 can inhibit the proliferation, migration and invasion of human endometrial cancer cells by targeting and inhibiting the expression of PTBP1.

Effect of triethanolamine on the biological characteristics of diffuse large B-cell lymphoma cells

ZHANG Gui-bing, HE Zheng-fei, SHANG Wen-zhong, WANG Ai-wei, WU Yan-fang, HUANG Hui-yan

2022, 20(5): 785-788. doi: 10.16766/j.cnki.issn.1674-4152.002454

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Objective To observe the effect of triethanolamine (TEOA) on the biological characteristics of diffuse large B-cell lymphoma (DLBCL) cells, so as to provide a basis for clinical treatment of the disease. Methods DLBCL cell lines OCI-LY3 were provided by the cell bank of the Chinese Academy of Sciences. They were randomised into the control group and observation group by random number table method, with five parallel holes in each group. Different concentrations (5, 10, 15, 20, 25, 30, 35, 40, 45, 50 μmol/L) of TEOA were added into the observation group and then cultured for 12 h. The GraphPad Prism 5 software was used to calculate the survival rates of DLBCL cells in both groups, flow cytometry was used to detect the apoptosis and invasion of DLBCL cells, the MTT method was used to detect the proliferation of DLBCL cells, and Western Blotting was used to detect the relative protein expression. The levels of reactive oxygen species (ROS) in both groups were compared, and research data were collected and input into the SPSS 20.0 statistical software for statistical analysis. Results After 24, 48 and 72 h of TEOA treatment, the cell proliferation rates in the observation group[(39.24±3.95)%, (57.08±5.87)% and (114.08±14.69)%] were significantly lower than those in the control group[(49.49±5.02)%, (95.12±9.64)% and (210.45±25.62)%, all P < 0.05]. The observation group had significantly smaller number of transmembrane cells (38.14±3.77) and significantly higher apoptosis rate[(12.14±1.24)%] than the control group[92.25±9.24 and (3.64±0.36)%, respectively, all P < 0.05]. TEOA could increase ROS levels in DLBCL OCI-LY3 cells and effectively upregulate the expression levels of p38, p-Chk1, p-Chk2 and γ-H2AX in OCI-LY3. Conclusion TEOA can inhibit cell proliferation and invasion whilst effectively inducing cell apoptosis and ROS expression. TEOA acting on DLBCL OCI-LY3 cells can promote the expression of p38, p-Chk1, p-Chk2 and γ-H2AX and induce the activation of the p38 MAPK pathway.

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2022 Vol. 20, No. 5

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