Objective To detect the expression of RNA-9 and RNA-210 in human normal ovarian cell line IOSE80, cancer cell line SKOV-3 and its cisplatin-resistant cell line SKOV-3/DDP; To investigate the effects of RNA-9 and RNA-210 on cell multiplication, apoptosis, migration and invasion on SKOV-3 and SKOV-3/DDP; Discuss probable functional mechanisms of RNA-9 and RNA-210 in serous ovarian cancer and provide a way for ovarian cancer targeted therapy finally.
Methods Using quantitative real-time PCR to assess the expression of RNA-9 and RNA-210 in normal ovarian cell line, ovarian cell line SKOV-3 and SKOV-3/DDP; Transfect RNA-9 mimics and RNA-210 mimics and its corresponding mimics negative control (mimics NC) into SKOV3 and SKOV-3/DDP respectively. CCK-8 assay, Annexin V/PI assay, Cell scratch experiment, cell invasion experiment were performed to evaluate the influence of overexpression of RNA-9 and RNA-210 on cell multiplication, apoptosis, migration and invasion.
Results Compared with normal ovarian cell, the expression of RNA-9 in SKOV-3 and SKOV-3/DDP was higher, and the expression of RNA-210 was lower; CCK-8 assay indicated that the ability of cell multiplication was decreased, Annexin V/PI assay indicated that the ability of cell apoptosis was promoted. Cell scratch experiment showed that the ability of cell migration were significantly decreased, the same with the ability of cell invasion, after Overexpressed either RNA-9 or RNA-210.
Conclusion Inclusions miR-9 and miR-210 plays a role of tumor suppressor gene in ovarian cancer cells; The ability of cell apoptosis can be increased by overexpressing the miR-9 or miR-210, and the cell multiplication, migration and invasion can be inhibited not only in ovarian cancer cell but also its cisplatin-resistant cell; miR-9 and miR-210 could be seen as new new potential therapeutic targets for ovarian cancer and recurrent ovarian cancer.
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