Relationship between ADAMS12 methylation and the incidence of bird breeder’s lung among Uygur pigeon breeder

Objective To detect the ADAMS12 methylation status of peripheral blood of Uygur pigeon breeder with bird breeder's disease, and explore the relationship between ADAMS12 methylation and bird breeder's disease. Methods Twenty cases of Uygur pigeon breeder with bird breeder's disease were assigned into case group, 20 cases of Uygur pigeon breeder without bird breeder's disease were assigned into control group A, and 20 healthy controls taking a physical examination were assigned into control group B. The data of all subjects were collected. The blood samples were prepared to extract DNA, after bisulfite conversion, PCR amplification, transcription in vitro and RNase a specific enzyme cut, parallel matrix assisted laser desorption ionization time of flight mass spectrometry mass spectrometry technique was used to detect ADAMS12 gene methylation. SPSS 20.0 software was used to compare the difference in ADAMS12 methylation rate among the three groups. Results Total 5 CpG loci (CpG_1,CpG_2,CpG_3,CpG_4,CpG_5) of ADAMS12 fragment were detected, the result showed that there were no statistically significant difference in methylation distribution among the three groups (χ² value were 0.915, 1.425, 0.389, 4.204, 4.758, all P>0.05). The difference in samples methylation distribution of 5 sites between every two groups was not statistically significant (all P>0.017). Conclusion There was no correlation between the methylation of ADAMS12 and the incidence of bird breeder's disease in Uygur Pigeon breeder.