Curative effect of Yunnan Baiyao on gingivitis and its influence on function of oral osteoclast

Objective To explore the curative effect of Yunnan Baiyao on gingivitis and its influence on the function of oral osteoclast. Methods A total of 100 cases of gingivitis treated in our hospital from June 2016 to June 2018 were randomly divided into the Yunnan Baiyao group and the control group with 50 cases in each group. Yunnan Baiyao group were given the Yunnan Baiyao solution to wash the affected part, and the control group was treated with minocycline hydrochloride ointment. Healthy and lesion area alveolar bone were collected for in vitro culture and subculture. The gingival index (GI), gingival groove haemorrhage index (SBI) and plaque index (PLI) was compared. The serum levels of tumor necrosis factor-alpha (TNFbeta), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8) and osteocalcin (OC) were measured by enzyme-linked immunosorbent assay. The morphology of oral osteoclasts was observed. The proliferation of oral osteoclasts was compared between the two groups. The mRNA expression of Type Ⅰ collagen was detected by immune transmission turbidimetry test. The level of alkaline phosphatase (ALP) was detected by double antibody sandwich method. Results After the treatment, the levels of GI, SBI and PLI in the Yunnan Baiyao group were lower than those of the control group (P < 0.05). After the treatment, the levels of TNF-beta, IL-1β, IL-6 and IL-8 in the Yunnan Baiyao group were lower than those in the control group (P < 0.05). At different time points, the proliferation rate and alkaline phosphatase activity of oral osteoclasts in the Yunnan Baiyao group were low compared with the control group, and the activity of tartaric acid acidic phosphatase was higher than that of the control group (P < 0.05). The mRNA level of type Ⅰ collagen protein, ALP activity and OC level in the Yunnan Baiyao group is lower than those in the control group after the treatment (P < 0.05). Conclusion Yunnan Baiyao can effectively inhibit the proliferation of osteoclasts in the oral cavity of gingivitis patients, promote differentiation, with a remarkable curative effect.