Influence of achyranthan on bone metabolism and biomechanical characteristics of elderly rats model of osteoporosis

Objective To explore the influence of achyranthan on bone metabolism and biomechanical characteristics of elderly osteoporosis model rats. Methods Totally 120 female SD rats were divided into 6 groups. In addition to the rats in the control group (Control) and the sham operation group (SO), the others were prepared for osteoporosis modes by surgical castration. After the surgery, the rats in intervention 1, 2, 3 were given intragastric administration with achyranthan of 100, 200, 400 mg/(kg·d), for 3 months. The levels of osteocalcin (OC), bone specific alkaline phosphatase (BAP), tartrate-resistant acid phosphatase (TPACP5b), Ⅰ type collagen cross-linking N-terminal peptide (NTX) and Ⅰ type collagen cross-linking C- terminal peptide (CTX) of abdominal venous blood of rats were detected by ELISA kits. The receptor activator of NF-kappa b (RANK), RANK ligand (RANKL) and osteoprotegerin (OPG) expression of myelosis of rats were detected by WB. Maximum stress and fracture absorb energy of centrum and thighbone of rats was detected by using servo-hydraulic materials testing machines. Results Compared with the Control/SO group, the serum OC and BAP level, and the medullispinal OPG and RANK expression of the model group decreased, and the serum TPACP5b, NTX and CTX level, and the medullispinal RANKL expression of the model group increased. Compared with model group, the serum OC and BAP level, and the medullispinal OPG and RANK expression of intervention 1, 2, 3 group decreased successively, and the serum TPACP5b, NTX and CTX level, and the medullispinal RANKL expression of intervention 1, 2, 3 group increased successively, the difference among the different groups was statistically significant (P＜0.05). Compared with the control/SO group, the maximum stress and fracture absorb energy of the model group decreased. Compared with model group, the maximum stress and fracture absorb energy of the intervention 1, 2, 3 group increased successively, the difference among the groups was statistically significant (P＜0.05). Conclusion Achyranthan can improve bone metabolism, increase bone density and bone compressive ability of elderly osteoporosis rats, and has potential application value in the field of anti-osteoporosis.