Saponins of Aralia Taibaiensis inhibits invasion and migration of HEC-1B cell line through down-regulating the expression of Sirtuin 1

Objective To explore the effects of Saponins of Aralia Taibaiensis(SAT) on the proliferation, migration and invasion of endometrial cancer cell HEC-1 B, and to provide a theoretical basis for the treatment of endometrial cancer. Methods HEC-1 B cells were treated with different concentrations SAT(0, 10, 20, 30 μmol/L). CCK8 assay was used to detect the cell proliferation, plate cell clone formation assay was used to detect colony formation, transwell chamber test was used to detect migration and invasion, and real-time PCR was used to detect the expression of sirtuin-1. The protein expressions of sirtuin 1, MMP-2 and MMP-9 were detected by Western Blotting. Results Compared with the control group, the cell proliferation, invasion and migration in SAT treatment group and the positive control group decreased significantly(all P<0.05), which was dose-dependent. Compared with the positive control group, the cell proliferation, invasion and migration in 20 and 30 μmol/L SAT group decreased significantly(all P<0.05). Compared with the control group, the levels of sirtuin 1 mRNA and protein, MMP-2 and MMP-9 protein in SAT treatment group and the positive control group decreased significantly, which was dose-dependent. Compared with the positive control group, the levels of sirtuin 1 mRNA and protein, MMP-2 and MMP-9 protein in 20 and 30 μmol/L SAT group decreased significantly(all P<0.05). Conclusion SAT can inhibit the migration and invasion of HEC-1 B cells, which may be related to the down-regulation of sirtuin-1 expression, and may provide a new possibility for the treatment of endometrial cancer.