Expression of miR-155 in villus tissues and its relationship with TIM3 level in patients with recurrent abortion

Objective To investigate the expression of miR-155 in villus tissues of recurrent abortion patients and its relationship with TIM3 level. Methods From August 2016 to March 2018, 63 cases of recurrent abortion patients underwent complete curettage of uterine cavity in Gynecology and Obstetrics Clinic of Hangzhou gynecology and obstetrics hospital were selected as the study group, at the same time, 57 cases of normal early pregnancy women who terminate the pregnancy by complete curettage of uterine cavity were selected as control group. The villus tissues of the two groups were collected to detect the expression levels of miR-155, TIM3, INF-γ, IL-4 and IL-13, and the relationship between miR-155 and TIM3, INF-γ, IL-4 and IL-13 of the study group were analyzed. Results The expression levels of miR-155, IL-4 and IL-13 mRNA in the study group were lower than those in the control group(all P<0.05), and the expression levels of TIM3, INF-γ and IL-2 mRNA were higher than those in the control group(all P<0.05). The expression level of TIM3 protein was higher than that of the control group(P<0.05). The miR155 of the study group was negatively correlated with TIM3, INF-γ and IL-2(r=-0.687,-0.636,-0.602, P<0.05), and positively correlated with IL-4 and IL-13(r=0.574, 0.498, P<0.05). TIM3 was positively correlated with INF-γ and IL-2, respectively(r=0.713, 0.644, P<0.05), and negatively correlated with IL-4 and IL-13(r=-0.615,-0.597, P<0.05). Conclusion The expression of miR-155 in villus tissues of patients with recurrent spontaneous abortion is decreased significantly, it is significantly negatively correlated with TIM3, they may interact with each other in the process of recurrent spontaneous abortion.