The Mechanism study of the promotion of photodynamic therapy combined with NS-398 on the apoptosis of bile duct cancer cells QBC939 through inhibiting Bcl-2

JIANG Hai-tao; HUANG Zuo-an; HUANG Dan-dan; XIANG Jian-jian; WANG Wu-ke; CHEN Yun-jie

doi:10.16766/j.cnki.issn.1674-4152.001765

Volume 19 Issue 2

Feb. 2021

Turn off MathJax

Article Contents

Article Navigation > Chinese Journal of General Practice > 2021 > 19(2): 193-197

JIANG Hai-tao, HUANG Zuo-an, HUANG Dan-dan, XIANG Jian-jian, WANG Wu-ke, CHEN Yun-jie. The Mechanism study of the promotion of photodynamic therapy combined with NS-398 on the apoptosis of bile duct cancer cells QBC939 through inhibiting Bcl-2[J]. Chinese Journal of General Practice, 2021, 19(2): 193-197. doi: 10.16766/j.cnki.issn.1674-4152.001765

Citation:

JIANG Hai-tao, HUANG Zuo-an, HUANG Dan-dan, XIANG Jian-jian, WANG Wu-ke, CHEN Yun-jie. The Mechanism study of the promotion of photodynamic therapy combined with NS-398 on the apoptosis of bile duct cancer cells QBC939 through inhibiting Bcl-2[J]. Chinese Journal of General Practice, 2021, 19(2): 193-197. doi: 10.16766/j.cnki.issn.1674-4152.001765

Citation:

JIANG Hai-tao, HUANG Zuo-an, HUANG Dan-dan, XIANG Jian-jian, WANG Wu-ke, CHEN Yun-jie. The Mechanism study of the promotion of photodynamic therapy combined with NS-398 on the apoptosis of bile duct cancer cells QBC939 through inhibiting Bcl-2[J]. Chinese Journal of General Practice, 2021, 19(2): 193-197. doi: 10.16766/j.cnki.issn.1674-4152.001765

PDF( 1589 KB)

The Mechanism study of the promotion of photodynamic therapy combined with NS-398 on the apoptosis of bile duct cancer cells QBC939 through inhibiting Bcl-2

doi: 10.16766/j.cnki.issn.1674-4152.001765

JIANG Hai-tao^{1, 2, 3}
, HUANG Zuo-an^{2, 3, 4}
, HUANG Dan-dan^{2, 3, 4}
, XIANG Jian-jian^{1, 2, 3}
, WANG Wu-ke^{1, 2, 3}
, CHEN Yun-jie^{1, 2, 3
,
,}

1.
Department of General Surgery, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, Zhejiang 315010, China

Funds:

2017HMKY11

2019HMKY67

2019E10020

2019A21003

Received Date: 2020-01-19
Available Online: 2022-02-19

Abstract

Abstract

Objective To study the effect of photodynamic therapy (PDT) combined with NS-398 on apoptosis of cholangiocarcinoma QBC939 cells and mechanism. Methods QBC939 cells were cultured and divided into four groups, namely, the photodynamic group, NS-398 group, joint experimental group and blank control group, which were exposed to 0, 2, 4, 6, 8 and 10 μg/mL of hematoporphyrin derivatives (HPD) with 0, 5, 10 and 15 J/cm² light radiation and 0, 25, 50, 100 and 200 μmol/L NS-398, respectively. The growth inhibition ratio of the QBC939 cells (R) was measured and calculated by cell proliferation experiment (CCK8). Flow cytometry was used to measure cell apoptosis. The mRNA and protein expression levels of Bcl-2 were measured by real-time PCR, immunocytochemistry and ELISA. Results Both PDT and NS-398 could inhibit the growth of QBC939 cells in vitro. When HPD concentration was 8 μ g/ml, light intensity was 5 J/cm², and NS-398 was 50 μmol/L, the R value was about 95%. There were significant differences between the combined experimental group and other groups (P < 0.05). If light radiation and NS-398 were increased, then the result would show no statistical differences. PDT combined with NS-398 could promote QBC939 cell apoptosis at the early stage through flow cytometry (F=3 224.753, P < 0.001). Real-time PCR demonstrated it could suppress the expression of Bcl-2 gene (F=6 262.227, P < 0.001). SP immunocytochemistry showed it could inhibit the expression of Bcl-2 (F=1 355.577, P < 0.001). ELISA showed it could inhibit the secretion of Bcl-2 (F=5 123.387, P < 0.001). Conclusion PDT combined with NS-398 could suppress the growth and promote early apoptosis of QBC939 cells. The inhibitory effect may be achieved by inhibiting the expression of Bcl-2 gene and protein, thus promoting the early apoptosis.
- Photodynamic therapy,
- Selective cyclooxygenase-2 inhibitor,
- Bile duct cancer,
- B-cell lymphoma-2,
- Apoptosis

FullText(HTML)

References(20)

References

[1]	OLIVEIRA I S, KILCOYNE A, EVERETT J M, et al. Cholangiocarcinoma: classification, diagnosis, staging, imaging features, and management[J]. Abdom Radiol, 2017, 42(6): 1637-1649. doi: 10.1007/s00261-017-1094-7
[2]	熊永福, 杨刚, 李强, 等. 胆管癌相关多原发癌临床特点及预后分析[J/CD]. 中华肝脏外科手术学电子杂志, 2018, 7(5): 396-401. doi: 10.3877/cma.j.issn.2095-3232.2018.05.012
[3]	邵子雨, 王许安, 刘颖斌. 肝内胆管癌的治疗现状及展望[J]. 浙江临床医学, 2019, 21(8): 1026-1028. https://www.cnki.com.cn/Article/CJFDTOTAL-ZPWL200102000.htm
[4]	王葵, 沈锋. 肝内胆管癌的诊治进展[J]. 肝胆外科杂志, 2019, 27(1): 1-6. https://www.cnki.com.cn/Article/CJFDTOTAL-GDWZ201901001.htm
[5]	刘金龙, 董家鸿. 肝门胆管癌临床治疗进展[J]. 河北医学, 2015, 21(6): 1005-1008. doi: 10.3969/j.issn.1006-6233.2015.06.047
[6]	ZHU A X. Future directions in the treatment of cholangiocarcinoma[J]. Best Pract Res Clin Gastroenterol, 2015, 29(2): 355-361. doi: 10.1016/j.bpg.2015.02.010
[7]	邹晓平, 丁希伟. 肝门部胆管癌的诊治进展及展望[J]. 中华消化杂志, 2018, 38(3): 151-154. doi: 10.3760/cma.j.issn.0254-1432.2018.03.003
[8]	CHEN Y J, JIANG H T, CAO J Y. Influence of photodynamic therapy on apoptosis and invasion of human cholangiocarcinoma QBC939 cell line[J]. Chin Med Sci J, 2015, 30(4): 252-259. doi: 10.1016/S1001-9294(16)30009-8
[9]	姜海涛, 陈云杰, 陆萍, 等. 选择性环氧合酶-2抑制剂NS-398和二十二碳六烯酸联合促进胆管癌QBC939细胞的凋亡[J]. 中华肝胆外科杂志, 2017, 23(8): 550-552. doi: 10.3760/cma.j.issn.1007-8118.2017.08.014
[10]	姜海涛, 周惟, 毛联钢, 等. 二十二碳六烯酸联合选择性环氧合酶-2抑制剂NS-398对人胆管癌QBC939细胞凋亡的影响[J]. 中华肝胆外科杂志, 2018, 24(5): 336-340. doi: 10.3760/cma.j.issn.1007-8118.2018.05.013
[11]	YAO C P, CAO X J, FU Z, et al. Boschniakia Rossica Polysaccharide Triggers Laryngeal Carcinoma Cell Apoptosis by Regulating Expression of Bcl-2, Caspase-3, and P53[J]. Med Sci Monit, 2017, 23: 2059-2064. doi: 10.12659/MSM.901381
[12]	GONG H, CHAO Y, XIANG J, et al. Hyaluronidase to enhance nanoparticle-based photodynamic tumor therapy[J]. Nano Lett, 2016, 16(4): 2512-2521. doi: 10.1021/acs.nanolett.6b00068
[13]	SHI X, ZHANG C Y, GAO J, et al. Recent advances in photodynamic therapy for cancer and infectious diseases[J]. Wiley Interdiscip Rev Nanomed Nanobiotechnol, 2019: e1560. doi: 10.1002/wnan.1560
[14]	陈越, 郑军, 谭潇. 光动力疗法在肿瘤治疗中的研究进展[J]. 实用医学杂志, 2019, 35(16): 2517-2521. doi: 10.3969/j.issn.1006-5725.2019.16.001
[15]	LAN M, ZHAO S, LIU W, et al. Photosensitizers for photodynamic therapy[J]. Advanced Healthcare Materials, 2019, 8(13): 1900132. doi: 10.1002/adhm.201900132
[16]	陈云杰, 姜海涛, 王天飞, 等. DHA和NS-398联合对人胆管癌QBC939细胞的抑制作用[J]. 中华全科医学, 2017, 15(11): 1860-1862, 1910. https://www.cnki.com.cn/Article/CJFDTOTAL-SYQY201711013.htm
[17]	欧阳国庆, 刘志鹏, 熊力, 等. 原卟啉介导的光动力疗法对结肠癌HCT116细胞的杀伤和促凋亡作用及其机制[J]. 中南大学学报(医学版), 2017, 42(8): 874-881. https://www.cnki.com.cn/Article/CJFDTOTAL-HNYD201708002.htm
[18]	姜海涛, 曹景玉. 光动力疗法在胆管癌治疗中的研究进展[J]. 中国普外基础与临床杂志, 2015, 22(5): 633-636. https://www.cnki.com.cn/Article/CJFDTOTAL-ZPWL201505035.htm
[19]	郝兴, 刘建生, 陈博艺, 等. 选择性环氧合酶-2抑制剂塞来昔布抗肿瘤作用机制的研究进展[J]. 中国现代医生, 2016, 54(34): 157-160. https://www.cnki.com.cn/Article/CJFDTOTAL-ZDYS201634047.htm
[20]	姜海涛, 张顺, 任燕萍, 等. NS-398和DHA联合抑制β-catenin、c-myc对胆管癌QBC939细胞凋亡的影响[J]. 中华普通外科杂志, 2018, 33(5): 416-419. doi: 10.3760/cma.j.issn.1007-631X.2018.05.015

Relative Articles

Supplements(0)

Cited By

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Figures(4) / Tables(2)

Get Citation

PDF

XML

Article Metrics

Article views (204) PDF downloads(1)

The Mechanism study of the promotion of photodynamic therapy combined with NS-398 on the apoptosis of bile duct cancer cells QBC939 through inhibiting Bcl-2

doi: 10.16766/j.cnki.issn.1674-4152.001765

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

The Mechanism study of the promotion of photodynamic therapy combined with NS-398 on the apoptosis of bile duct cancer cells QBC939 through inhibiting Bcl-2

doi: 10.16766/j.cnki.issn.1674-4152.001765

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Export File

Citation

Format

Content