Volume 19 Issue 3
Mar.  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(3): 387-390
YANG Hui-fang, ZHAO Li, LU Ling-song, WU Yan-hui. Expression of GFAP and inflammatory factors in spinal cord of neuropathic pain rats treated with glucocorticoid receptor agonists[J]. Chinese Journal of General Practice, 2021, 19(3): 387-390. doi: 10.16766/j.cnki.issn.1674-4152.001817
Citation: YANG Hui-fang, ZHAO Li, LU Ling-song, WU Yan-hui. Expression of GFAP and inflammatory factors in spinal cord of neuropathic pain rats treated with glucocorticoid receptor agonists[J]. Chinese Journal of General Practice, 2021, 19(3): 387-390. doi: 10.16766/j.cnki.issn.1674-4152.001817
shu

Expression of GFAP and inflammatory factors in spinal cord of neuropathic pain rats treated with glucocorticoid receptor agonists

doi: 10.16766/j.cnki.issn.1674-4152.001817
  • 1.

    Department of Anesthesiology, Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, China

Funds:

 2016KYA156

  • Received Date: 2020-02-25
    Available Online: 2022-02-19
    Abstract
  •   Objective  To investigate the effect of glucocorticoid receptor agonists on glial fibrillary acidic protein (GFAP) and inflammatory factors expression in spinal cord of neuropathic pain rats.  Methods  Twenty SD rats were randomly divided into control group, sham operation group, model group and treatment group. The model group and treatment group were used to establish neuropathic pain model. In the sham operated group, only the sciatic nerve was exposed, but not ligated. Dexamethasone was injected intrathecally in the treatment group, normal saline was injected intrathecally in other three groups for 7 days. The mechanical pain threshold was measured by Von Frey filament one day before operation, three days after operation and seven days after operation. The rats were killed 7 days after operation. The expression of GFAP protein was detected by western blot and the expression of inflammatory factor was detected by RT-PCR.  Results  The mechanical pain threshold and thermal pain threshold in the treatment group were significantly lower than those in the control group and the sham operation group (P < 0.05), but significantly higher than those in the model group (P < 0.05). The expression level of GFAP protein in the model group and the treatment group was significantly higher than that in the control group and the sham operation group (P < 0.05), but the expression level of GFAP protein in the treatment group was significantly lower than that in the model group (P < 0.05). The expression of TNF-α and IL-1β mRNA in the spinal cord of the model group and the treatment group was significantly higher than that of the control group and the sham operation group (P < 0.05), but the expression of TNF-α and IL-1β mRNA in the spinal cord of the treatment group was significantly lower than that of the model group (P < 0.05).  Conclusion  Glucocorticoid receptor agonists can significantly reduce the pain sensitivity of neuropathic pain rats, and its mechanism may be related to the down-regulation of GFAP and the expression of downstream inflammatory factors.

     

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