Volume 19 Issue 3
Mar.  2021
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(3): 405-409
LI Li-feng, YANG Hua-jun, ZHANG Qiao-li, LAI Yue-li, ZHAO Tong. Zhuhuang compound preparation promotes the healing of soft and bone wounds in cellular level[J]. Chinese Journal of General Practice, 2021, 19(3): 405-409. doi: 10.16766/j.cnki.issn.1674-4152.001822
Citation: LI Li-feng, YANG Hua-jun, ZHANG Qiao-li, LAI Yue-li, ZHAO Tong. Zhuhuang compound preparation promotes the healing of soft and bone wounds in cellular level[J]. Chinese Journal of General Practice, 2021, 19(3): 405-409. doi: 10.16766/j.cnki.issn.1674-4152.001822
shu

Zhuhuang compound preparation promotes the healing of soft and bone wounds in cellular level

doi: 10.16766/j.cnki.issn.1674-4152.001822

  • Department of Stomatology, Fuyang People's Hospital, Hangzhou, Zhejiang 311400, China

Funds:

 2018ZA105

  • Received Date: 2020-09-27
    Available Online: 2022-02-19
    Abstract
  •   Objective  To demonstrate the effectiveness of the traditional compound preparation of Zhuhuang powder in promoting wound healing of soft and bone as well as to explore the quantitative effect relationship of 5 kinds of traditional Chinese medicines in compound in the prescription, and provide scientific basis for the new clinical application of ancient Chinese medicines and screen new prescriptions.  Methods  Zhuhuang powder was used as the basic prescription, and Panax notoginseng, elderberry and intermittent medicinal materials were added. Five formulations were obtained by using uniform design method to proportionate the dosage of medicinal materials. The water extract and ethanol extract dry powder were prepared from five groups of prescriptions. RAW 309 Cr.1 cells stimulated with LPS were treated with these water extracts or ethanol extracts to detect inflammatory factors (NO) levels. The proliferation of human umbilical vein endothelial cells (HUVEC-12) and osteoblasts (UMR-106) were detected by methyl thiazolyl tetrazolium (MTT).  Results  The extract 2, 3 and 5 of 5 groups showed 44.3%-56.7% inhibition of NO expression at 1-5 μg/mL. The extract 1 and 4 showed 46.7%-73.4% inhibition of NO expression at 1-5 μg/mL. The extract 4 showed 110%-128% induction of the proliferation of HUVEC-12 cells at 3-4 μg/mL. The extract 1 showed 110%-120% proliferation at 4 μg/mL. Compared with control, all 5 groups showed 18% induction on the proliferation of UMR-106 cells, the proliferation effect was obvious at 4 μg/mL.  Conclusion  The five formulations showed single or multiple effects on cell proliferation in vitro. Formula No.4 (pearl 8 g, taurine 8 g, notoginseng 6 g, intermittent 15 g, elderberry 9 g) shows inhibition of inflammatory factors release and strong proliferation activity of human umbilical vein endothelial cells and osteoblasts (P < 0.05). The traditional prescription of Zhuhuang powder can promote the healing of soft tissue and bone tissue wounds. Formula 4 inhibits the release of inflammatory factors and has a significant effect on the proliferation of human umbilical vein endothelial cells and osteoblasts.

     

    • FullText(HTML)
  • loading
    • References(15)
  • [1]
    米施. 现代口腔种植学[M]. 李德华, 译. 北京: 人民军医出版社, 2015: 4-21.
    [2]
    刘征宇. 具有促进骨折愈合的中药及作用机制[J]. 世界中医药, 2019, 14(10): 2809-2813. doi: 10.3969/j.issn.1673-7202.2019.10.058
    [3]
    杨洪杰, 周利, 吴海红, 等. 清热解毒中药外敷治疗外伤感染创面的疗效观察及作用机制研究[J]. 中医正骨, 2016, 28(8): 13-18, 23. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYZG201608003.htm
    [4]
    冷方南. 中国基本中成药[M]. 北京: 人民卫生出版社, 1994: 443.
    [5]
    周煜虎, 段明明, 曹强. 续断醇提物对骨关节炎的治疗作用及相关分子机制的研究[J]. 中国中医骨伤科杂志, 2020, 28(7): 1-5. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZG202007001.htm
    [6]
    杭传珍. 活血化淤治则的药理学解除[J]. 中医临床研杂志, 2019, 11(1): 34-35.
    [7]
    中华人民共和国药典委员会. 中国药典[M]. 北京: 中国医药科技出版社, 2020: 1426-1430.
    [8]
    梁志民. 探究均匀设计法在药物制剂领域中的应用及问题[J]. 世界最新医学信息文摘, 2019, 19(52): 284, 309. https://www.cnki.com.cn/Article/CJFDTOTAL-WMIA201952200.htm
    [9]
    HWANG D, KANG M J, JO M J, et al. Anti-inflammatory activity of beta-thymosin peptide derived from pacific oyster(Crassostrea gigas) on NO and PGE2 production by down-regulating NF-kappaB in LPS-induced RAW264.7 macrophage cells[J]. Mar Drugs, 2019, 17(2): 129. doi: 10.3390/md17020129
    [10]
    GUTIERREZ R M P, HOYO-VADILLO C. Anti-inflammatory potential of petiveria alliacea on activated RAW264.7 murine macrophages[J]. Pharmacogn Mag, 2017, 13(Suppl 2): S174-S178. http://europepmc.org/abstract/MED/28808377
    [11]
    ZOU Y H, ZHAO L, XU Y K. Anti-inflammatory sesquiterpenoids from the Traditional Chinese Medicine Salvia plebeia: Regulates pro-inflammatory mediators through inhibition of NF-kappaB and Erk1/2 signaling pathways in LPS-induced Raw264.7 cells[J]. J Ethnopharmacol, 2018, 210: 95-106. doi: 10.1016/j.jep.2017.08.034
    [12]
    GHASEMI M. Nitric oxide: Antidepressant mechanisms and inflammation[J]. Adv Pharmacol, 2019, 86: 121-152. http://www.ncbi.nlm.nih.gov/pubmed/31378250
    [13]
    吴彦秋, 陈宣好, 代佑罡, 等. 氟化钠对大鼠成骨肉瘤细胞UMR-106成骨活性标志物的影响[J]. 现代预防医学, 2018, 45(5): 883-886, 891. https://www.cnki.com.cn/Article/CJFDTOTAL-XDYF201805030.htm
    [14]
    许锦心, 陈国栋, 伍倩琪, 等. 淫羊藿苷对骨质疏松状态下牙种植体周围骨组织内BMP2含量的影响[J]. 全科口腔医学电子杂志, 2018, 5(36): 91-93. doi: 10.3969/j.issn.2095-7882.2018.36.063
    [15]
    万长青, 陈学群, 文燕. 中药对糖尿病大鼠牙种植骨整合的骨组织形态学影响分析[J]. 临床医学工程, 2016, 23(6): 711-712. doi: 10.3969/j.issn.1674-4659.2016.06.0711
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(3)  / Tables(4)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (215) PDF downloads(0) Cited by()
    Proportional views
    Related

    Address：No. 287, Changhuai Road, Bengbu, Anhui Province, 233004, P.R.ChinaphoneNo：0552-3066635； 0552-3051890Email：zhqkyx@163.com

    Copyright © Chinese Journal of General Practice皖ICP备2020018345号-2

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return