Volume 19 Issue 4
Apr.  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(4): 547-549, 647
CAO Zhen-xue, CUO Yuan-yuan, LIU Bei-bei, SUN Wei, GAO Wu-yue, LIANG Yu-jie, LIU Jian-min. Correlation analysis of mir-15a and postoperative recurrence amongst patients with bladder carcinoma[J]. Chinese Journal of General Practice, 2021, 19(4): 547-549, 647. doi: 10.16766/j.cnki.issn.1674-4152.001857
Citation: CAO Zhen-xue, CUO Yuan-yuan, LIU Bei-bei, SUN Wei, GAO Wu-yue, LIANG Yu-jie, LIU Jian-min. Correlation analysis of mir-15a and postoperative recurrence amongst patients with bladder carcinoma[J]. Chinese Journal of General Practice, 2021, 19(4): 547-549, 647. doi: 10.16766/j.cnki.issn.1674-4152.001857
shu

Correlation analysis of mir-15a and postoperative recurrence amongst patients with bladder carcinoma

doi: 10.16766/j.cnki.issn.1674-4152.001857

  • Department of Urology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 1808085QH279

  • Received Date: 2020-11-09
    Available Online: 2022-02-16
    Abstract
  •   Objective  This study aimed to investigate the correlation between the miR-15a expression level and progression of bladder cancer and postoperative recurrence in patients with bladder cancer.  Methods  Thirty-two patients diagnosed with bladder cancer who underwent transurethral plasma resection of bladder tumour transurethral resection of bladder tumour (TURBT) in the Department of Urology of the First Affiliated Hospital of Bengbu Medical College from June 2014 to December 2019 were enrolled in this study. Bladder cancer tissue was obtained from the tumour site, whereas paracancerous tissue was obtained from the adjacent normal area. Real-time quantitative polymerase chain reaction was used to detect the expression of miR-15a in bladder cancer tissue samples or paracancerous tissue samples. The Kaplan-Meier method was used to describe the postoperative prognosis curve, and log-rank test was used for comparison amongst groups. The χ2-test test was used to compare the differences in the expression level and general clinical characteristics of miR-15a in bladder cancer tissues and matched paracancerous tissues. Multivariate logistic regression analysis was used to describe the clinicopathological features such as muscular invasion, distant metastasis and tumour grade.  Results  The expression level of miR-15a in bladder cancer tissues was significantly lower than that in adjacent tissues (1.126±0.254 vs 1.450±0.139), and the difference was statistically significant (P < 0.001). In addition, the miR-15a expression level was significantly correlated with muscular invasion (P=0.001), distant metastasis (P=0.005) and tumour grade (P=0.027). Log-rank test showed that patients with down-regulated miR-15a expression had a higher overall recurrence rate, and the difference was statistically significant (P=0.023). Multivariate logistic regression analysis showed that positive distant metastasis, muscular invasion (T2-T4) and tumour grade G3 were independent risk factors for the down-regulation of miR-15a expression.  Conclusion  The expression level of miR-15a may be closely related to the occurrence and development of bladder cancer, and its expression level is significantly correlated with muscular invasion, distant metastasis and tumour grade. The down-regulated expression level of miR-15a may be a biomarker for bladder tumour recurrence.

     

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