Volume 19 Issue 5
May  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(5): 867-870
ZHANG Yao-yao, WU Qiang, GAO Shan, HOU Ting-ting, WANG Hua-xue, ZHANG Feng, ZHANG Yong. Alveolar macrophage phenotypes in bronchoalveolar lavage fluid from sepsis[J]. Chinese Journal of General Practice, 2021, 19(5): 867-870. doi: 10.16766/j.cnki.issn.1674-4152.001937
Citation: ZHANG Yao-yao, WU Qiang, GAO Shan, HOU Ting-ting, WANG Hua-xue, ZHANG Feng, ZHANG Yong. Alveolar macrophage phenotypes in bronchoalveolar lavage fluid from sepsis[J]. Chinese Journal of General Practice, 2021, 19(5): 867-870. doi: 10.16766/j.cnki.issn.1674-4152.001937
shu

Alveolar macrophage phenotypes in bronchoalveolar lavage fluid from sepsis

doi: 10.16766/j.cnki.issn.1674-4152.001937
  • 1.

    Department of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 1804h08020287

  • Received Date: 2020-12-01
    Available Online: 2022-02-16
    Abstract
  •   Objective   Sepsis is an immune disorder and an infectious disease with high mortality. As important antigen-presenting cells, macrophages play an indispensable role in innate immunity and adaptive immunity. Macrophages may polarise into two distinct phenotypes presenting as M1 and M2 when cells accept different stimuli. M1 macrophages and M2 macrophages enhance inflammation and tissue repair and remodelling, respectively. This study aimed to observe changes in alveolar macrophage (AMs) phenotypes in the bronchoalveolar lavage fluid (BALF) of patients with sepsis and analyse the correlation between the two distinct AM phenotypes and prognosis of patients with sepsis.   Methods   Twenty-nine patients who experienced consecutive sepsis and admitted to the intensive care unit of the Department of Pulmonary and Critical Care Medicine from July 2020 to December 2020 were included in this study. These patients underwent bronchoalveolar lavage on the first and the seventh days after the admission. The proportion of AMs in BALF was assessed via flow cytometry by using three colour fluorescent stains. CD68 was used as the marker of, total AM whereas CD86 and CD206 were used as M1 and M2 AM markers, respectively. The basic clinical data of the patients were collected and analysed. They were divided into a survival group and a non-survivor group according to mortality data within 28 days.   Results   The morphological structures of the M1 and M2 AMs were different. Flow cytometry revealed that the M1 AMs have a larger cell size and granularity than the M1 AMs. In the survivor group, the proportion of M1 and M2 in BALF were (21.39±9.91)% and (4.66±2.53)%, respectively, on the first day. On the seventh day, the proportion of M1 and M2 in BALF were (3.62±3.31)% and (44.41±7.47)%, respectively. The percentage of M2 and M1 AMs from BALF increased and decreased, respectively, on the seventh day compared with that on the first day (all P < 0.05). In the nonsurvivor group, the proportion of M1 and M2 in BALF were (18.37±2.75)% and (20.40±2.27)%, respectively, on the first day and (7.00±1.98)% and (19.69±4.24)%, respectively, on the seventh day., the percentage of M1 decreased, whereas that of M2 continued to increase compared with that of M1 (all P < 0.05). No statistically significant differences were observed between the first and the seventh days (P>0.05).   Conclusion   Early dynamic monitoring of changes in AM phenotypes can help predict the prognosis of patients with sepsis within 28 days.

     

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