Effect of myosin light chain regulated by cardiac myosin light chain kinase on cardiac hypertrophy

HE Mingyuan; GE Junfeng; HUANG Ling; WANG Shixiang; YAN Yi

doi:10.16766/j.cnki.issn.1674-4152.002034

Volume 19 Issue 8

Aug. 2021

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Article Navigation > Chinese Journal of General Practice > 2021 > 19(8): 1257-1261, 1402

HE Mingyuan, GE Junfeng, HUANG Ling, WANG Shixiang, YAN Yi. Effect of myosin light chain regulated by cardiac myosin light chain kinase on cardiac hypertrophy[J]. Chinese Journal of General Practice, 2021, 19(8): 1257-1261, 1402. doi: 10.16766/j.cnki.issn.1674-4152.002034

Citation:

HE Mingyuan, GE Junfeng, HUANG Ling, WANG Shixiang, YAN Yi. Effect of myosin light chain regulated by cardiac myosin light chain kinase on cardiac hypertrophy[J]. Chinese Journal of General Practice, 2021, 19(8): 1257-1261, 1402. doi: 10.16766/j.cnki.issn.1674-4152.002034

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Effect of myosin light chain regulated by cardiac myosin light chain kinase on cardiac hypertrophy

doi: 10.16766/j.cnki.issn.1674-4152.002034

HE Mingyuan^{1, 2, 3}
, GE Junfeng^{1, 3}
, HUANG Ling^{1, 3}
, WANG Shixiang¹
, YAN Yi^{1, 2, 3
,
,}

1.
Department of Cardiology, the Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510150, China

Funds:

81870320

81600349

201803

201804010008

TS20190337

pdjha0413

Received Date: 2021-01-16
Available Online: 2022-02-16

Abstract

Abstract

Objective To investigate the effect of cardiac myosin light chain kinase (cMLCK) on the pathological cardiac hypertrophy induced by AngⅡ. Methods The cMLCK was purified and the active sites of cMLCK were explored in vitro. Wild type cMLCK (WT cMLCK) and its N-terminal knockout variants (ΔNT-cMLCK) adenovirus were prepared. The primary cardiomyocytes were infected and randomly divided into 6 groups: control group, AngⅡ group, WT cMLCK group, WT cMLCK+AngⅡ group, Δ NT cMLCK group, Δ NT cMLCK+AngⅡ group, 3 samples in each group. The expression of ANP and β-MHC, which are the heart failure relative factor, was detected by RT-PCR. The expression of cMLCK and MLC2v were detected by western blot. The area of cardiomyocytes was detected by immunofluorescence. Results WT-cMLCK could phosphorylated MLC2v effectively, which was abrogated in ΔNT-cMLCK. After treated with AngⅡ, the cell area [(1 787.0±142.6) μm² vs. (2 458.0±211.3) μm², P < 0.001] and expression of ANP and β-MHC in neonatal rat cardiomyocyte (NRCM) increased (all P < 0.05). The expression of cMLCK and p-MLC2v were upregulated (all P < 0.05). However, the cardiac hypertrophy induced by AngⅡ was reversed by WT-cMLCK overexpression [(2 527.0±116.4) μm² vs. (1 775.0±88.5) μm², P < 0.001], while ΔNT-cMLCK overexpression group lost its protective effect [(1 775.0±88.5) μm² vs. (2 613.0±118.4) μm², P < 0.001]. Conclusion The protective effect of cMLCK to pathological cardiac hypertrophy induced by AngⅡ depends on its N terminal.
- Cardiac myosin light chain kinase,
- Cardiac myosin light chain,
- Cardiac hypertrophy

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References

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Effect of myosin light chain regulated by cardiac myosin light chain kinase on cardiac hypertrophy

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