Volume 19 Issue 9
Sep.  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(9): 1455-1458,1473
LI Xing, ZHU Zhen-hua, WU Zhong-min, DONG Liang, YE Bin, CAI He-fei. Improved effect of PPARγ activator on bleomycin-induced pulmonary fibrosis in mice[J]. Chinese Journal of General Practice, 2021, 19(9): 1455-1458,1473. doi: 10.16766/j.cnki.issn.1674-4152.002084
Citation: LI Xing, ZHU Zhen-hua, WU Zhong-min, DONG Liang, YE Bin, CAI He-fei. Improved effect of PPARγ activator on bleomycin-induced pulmonary fibrosis in mice[J]. Chinese Journal of General Practice, 2021, 19(9): 1455-1458,1473. doi: 10.16766/j.cnki.issn.1674-4152.002084
shu

Improved effect of PPARγ activator on bleomycin-induced pulmonary fibrosis in mice

doi: 10.16766/j.cnki.issn.1674-4152.002084
  • 1.

    Department of Pediatrics, Taizhou Central Hospital (Affiliated Hospital of Taizhou University), Taizhou, Zhejiang 318000, China

Funds:

 82072146

 2018C37108

  • Received Date: 2020-09-29
    Available Online: 2022-02-15
    Abstract
  •   Objective  To explore the effect of peroxisome proliferator-activated receptor-γ (PPARγ) activator on bleomycin-induced pulmonary fibrosis in mice and its related mechanisms.   Methods  Thirty-six BALB/c mice were randomly divided into control group, model group and PPARγ-activated group, 12 mice in each group. Except for the control group, the other groups of mice were injected intratracheally with 5 mg/kg bleomycin to induce pulmonary fibrosis. After 24 h, the mice in the PPARγ-activated group were orally administered with 100 mg/kg rosiglitazone solution. The lung coefficient was calculated. The contents of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), hydroxyproline (HYP) and transforming growth factor-β1 (TGF-β1) in lung tissue were detected by ELISA. HE and masson staining were used to observe the pathological changes of lung tissue. The contents of PPARγ, peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α) and nuclear factor erythroid-2-related factor (Nrf2) protein in lung tissue were detected by western blot.   Results  The lung coefficient, Ashcroft score of lung tissue, and the levels of HYP, TGF-β1 and MDA in the model group were significantly higher than those in the control group and PPARγ-activated group (all P < 0.05). The levels of SOD and GPx in the lung tissues of the model group were significantly lower than those of the control group and PPARγ-activated group (all P < 0.05). Compared with the model group, the expression of PPARγ, PGC-1α, and Nrf2 proteins in the lung tissue of rats in the PPARγ activation group increased significantly (all P < 0.05).   Conclusion  PPARγ activator can inhibit bleomycin-induced lung fibrosis in mice, which may be related to reducing inflammation and oxidative stress.

     

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